(24S,25)-Epoxycholesterol

Cat. No.: HY-W040150 Purity: 98.1%: FAQs
Data Sheet SDS COA Handling Instructions

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24S,25-Epoxycholesterol is an agonist for Liver X Receptor (LXR). 24S,25-Epoxycholesterol exhibits properties in regulating the cholesterol efflux, inhibiting tumor growth against gastric cancer and glioblastoma and inducing apoptosis in BMMC cells.

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(24S,25)-Epoxycholesterol Chemical Structure

CAS No. : 77058-74-3

Size	Price	Stock	Quantity
1 mg	USD 520	In-stock	Estimated Time of Arrival: December 31
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10 mg		Get quote

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Purity & Documentation
References
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Description

24S,25-Epoxycholesterol is an agonist for Liver X Receptor (LXR). 24S,25-Epoxycholesterol exhibits properties in regulating the cholesterol efflux^[1], inhibiting tumor growth against gastric cancer and glioblastoma^[2]^[3] and inducing apoptosis in BMMC cells^[5].

Cellular Effect

Cell Line	Type	Value	Description	References
CV-1	EC₅₀	117 nM Compound: 1	Agonist activity at human LXRalpha coexpressed in African green monkey CV1 cells by Gal4 responsive reporter gene assay Agonist activity at human LXRalpha coexpressed in African green monkey CV1 cells by Gal4 responsive reporter gene assay	[PMID: 26990539]
CV-1	EC₅₀	168 nM Compound: 1	Agonist activity at human LXRbeta coexpressed in African green monkey CV1 cells by Gal4 responsive reporter gene assay Agonist activity at human LXRbeta coexpressed in African green monkey CV1 cells by Gal4 responsive reporter gene assay	[PMID: 26990539]
SH-SY5Y	EC₅₀	0.4 μM Compound: 1	Agonist activity at human LXRbeta expressed in human SH-SY5Y cells co-transfected with Gal4-LBD after 24 hrs by luciferase reporter gene assay Agonist activity at human LXRbeta expressed in human SH-SY5Y cells co-transfected with Gal4-LBD after 24 hrs by luciferase reporter gene assay	[PMID: 19264481]

In Vitro

24S,25-Epoxycholesterol (1-10 μM) upregulates the LXR-related genes ABCA1, ABCG1 and APOE, promotes the cholesterol efflux, therefore prevents the foam cell formation and blocks proliferation of mouse and human glioma stem-like cells through depeting cellular cholesterol^[1]^[3].
24S,25-Epoxycholesterol inhibits proliferation and migration of HGC27, which is enhanced by knockout of LXRβ^[2].
24S,25-Epoxycholesterol (40 μM) inhibits HMG-CoA reductase and activates the LXR, which suppresses mevalonate-dependent isoprenoid production and enhancing the ATP-binding cassette G1 expression, induces apoptosis in bone marrow-derived murine mast cells (BMMCs)^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:	HGC27
Concentration:	1 μM
Incubation Time:	18 h
Result:	Inhibited proliferation of HGC27

Cell Migration Assay ^[2]

Cell Line:	HGC27
Concentration:	1 μM
Incubation Time:	18 h
Result:	Reduced HGC27 migration.

In Vivo

24S,25-Epoxycholesterol (5 mM, icv) enhances the mDA neurogenesis, limits the neurodegeneration in CYP46A1-overexpressing mice midbrain^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	GGPP induced mDA neurogenesis defect in CYP46A1 overexpressing CD-1 mice^[4]
Dosage:	5 mM
Administration:	icv, 1 μL, single dosage
Result:	Increased levels of mDA neurons, blocked the GGPP induced decrease of double EdU and TH cells.

Molecular Weight

400.64

Formula

C₂₇H₄₄O₂

CAS No.

77058-74-3

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@](CC[C@]2([H])[C@H](C)CC[C@H]3C(C)(O3)C)([H])[C@@]4([H])[C@@](CC1)([H])[C@@]5(C(C[C@H](CC5)O)=CC4)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

Ethanol : 16 mg/mL (39.94 mM; Need ultrasonic and warming)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4960 mL	12.4800 mL	24.9601 mL
5 mM	0.4992 mL	2.4960 mL	4.9920 mL
10 mM	0.2496 mL	1.2480 mL	2.4960 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.1%

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Data Sheet (274 KB) SDS (393 KB)

COA (247 KB)

Handling Instructions (2659 KB)

References

[1]. Beyea MM, et al., Selective up-regulation of LXR-regulated genes ABCA1, ABCG1, and APOE in macrophages through increased endogenous synthesis of 24(S),25-epoxycholesterol. J Biol Chem. 2007 Feb 23;282(8):5207-16. [Content Brief]

[2]. Guo F, et al., Upregulation of 24(R/S),25-epoxycholesterol and 27-hydroxycholesterol suppresses the proliferation and migration of gastric cancer cells. Biochem Biophys Res Commun. 2018 Oct 12;504(4):892-898. [Content Brief]

[3]. Nguyen TP, et al., Selective and brain-penetrant lanosterol synthase inhibitors target glioma stem-like cells by inducing 24(S),25-epoxycholesterol production. Cell Chem Biol. 2023 Feb 16;30(2):214-229.e18. [Content Brief]

[4]. Theofilopoulos S, et al., 24(S),25-Epoxycholesterol and cholesterol 24S-hydroxylase (CYP46A1) overexpression promote midbrain dopaminergic neurogenesis in vivo. J Biol Chem. 2019 Mar 15;294(11):4169-4176. [Content Brief]

[5]. Fukunaga M, et al., Mast cell death induced by 24(S),25-epoxycholesterol. Exp Cell Res. 2010 Nov 15;316(19):3272-81. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol	1 mM	2.4960 mL	12.4800 mL	24.9601 mL	62.4002 mL
	5 mM	0.4992 mL	2.4960 mL	4.9920 mL	12.4800 mL
	10 mM	0.2496 mL	1.2480 mL	2.4960 mL	6.2400 mL
	15 mM	0.1664 mL	0.8320 mL	1.6640 mL	4.1600 mL
	20 mM	0.1248 mL	0.6240 mL	1.2480 mL	3.1200 mL
	25 mM	0.0998 mL	0.4992 mL	0.9984 mL	2.4960 mL
	30 mM	0.0832 mL	0.4160 mL	0.8320 mL	2.0800 mL