28-Homobrassinolide

Name: 28-Homobrassinolide
Brand: MedChemExpress
SKU: HY-N9435
Rating: 4.5 (1 reviews)

Cat. No.: HY-N9435 Purity: 96.96%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

28-Homobrassinolide is a phytosteroid. 28-Homobrassinolide can be used for the research of cholesterol and glucose homeostasis.

For research use only. We do not sell to patients.

28-Homobrassinolide Chemical Structure

CAS No. : 82373-95-3

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 54	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 54	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 390	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

28-Homobrassinolide is a phytosteroid. 28-Homobrassinolide can be used for the research of cholesterol and glucose homeostasis^[1].

In Vitro

28-Homobrassinolide (5-20 μM) indicates a 2-fold increase in glucose utilization and ABCA1 and SREBP2 protein expression in HepG2 cells^[1].
28-Homobrassinolide decreases tissue glucose and cholesterol levels, increases cholesterol level and tissue hexokinase activity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:	HepG2 cells
Concentration:	1 µM, 5 µM, 10 µM, 20 µM, 30 µM, 40 µM, and 80 µM
Incubation Time:	24 h
Result:	The IC₅₀ value of 40.57µM obtained by the MTT assay.

Western Blot Analysis^[1]

Cell Line:	HepG2 cells
Concentration:	5, 10, and 20 μM
Incubation Time:	12 h
Result:	Exhibited an increase in both ABCA1 and SREBP2 markers.

In Vivo

28-HB (1-20 μg/day; for 6 weeks) exhibites a marked decrease in aortic fat deposit and serum marker levels in high-fat diet-fed C57BL/6 mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	High-fat diet-fed C57BL/6 mice^[1]
Dosage:	1-20 μg/day
Administration:	For 6 weeks
Result:	Observed a significant decrease in lipid deposition.

Molecular Weight

494.70

Formula

C₂₉H₅₀O₆

CAS No.

82373-95-3

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@](CC[C@]2([H])[C@H](C)[C@@H](O)[C@H](O)[C@@H](CC)C(C)C)([H])[C@@]3([H])[C@@](CC1)([H])[C@@]4([C@@](C[C@@H]([C@@H](C4)O)O)([H])C(OC3)=O)C

Initial Source

Plants

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 20 mg/mL (40.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0214 mL	10.1071 mL	20.2143 mL
5 mM	0.4043 mL	2.0214 mL	4.0429 mL
10 mM	0.2021 mL	1.0107 mL	2.0214 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 96.96%

Select Batch:

Data Sheet (278 KB) SDS (251 KB)

COA (257 KB) RP-HPLC (178 KB)

Handling Instructions (2659 KB)

References

[1]. Victor Mukherjee, et al. Phytosteroid 28-homobrassinolide targets cholesterol and glucose homeostasis implicating ABCA1 and SREBP role in regulation. Steroids. 2021 Jan;165:108756. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0214 mL	10.1071 mL	20.2143 mL	50.5357 mL
	5 mM	0.4043 mL	2.0214 mL	4.0429 mL	10.1071 mL
	10 mM	0.2021 mL	1.0107 mL	2.0214 mL	5.0536 mL
	15 mM	0.1348 mL	0.6738 mL	1.3476 mL	3.3690 mL
	20 mM	0.1011 mL	0.5054 mL	1.0107 mL	2.5268 mL
	25 mM	0.0809 mL	0.4043 mL	0.8086 mL	2.0214 mL
	30 mM	0.0674 mL	0.3369 mL	0.6738 mL	1.6845 mL
	40 mM	0.0505 mL	0.2527 mL	0.5054 mL	1.2634 mL