3-Acetamidocoumarin

Cat. No.: HY-W014750 Purity: 99.86%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

3-Acetamidocoumarin plays an important role in biology and medicine. 3-Acetamidocoumarin has physiological effects and has been used for many diseases such as research of burns, brucellosis-rheumatic diseases and cancer.

For research use only. We do not sell to patients.

3-Acetamidocoumarin Chemical Structure

CAS No. : 779-30-6

Size	Price	Stock	Quantity
25 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Biological Activity
Purity & Documentation
References
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Description

Molecular Weight

203.19

Formula

C₁₁H₉NO₃

CAS No.

779-30-6

Appearance

Solid

Color

White to off-white

SMILES

O=C1OC2=C(C=C1NC(C)=O)C=CC=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 12.5 mg/mL (61.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.9215 mL	24.6075 mL	49.2150 mL
5 mM	0.9843 mL	4.9215 mL	9.8430 mL
10 mM	0.4922 mL	2.4608 mL	4.9215 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (6.15 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (6.15 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.86%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (250 KB) HNMR (255 KB) LCMS (98 KB)

Handling Instructions (2659 KB)

References

[1]. ErolTaşal, MuratTanışlı. A comparison for application time of electrical discharge onto 3-acetamidocoumarin molecule. J Mol Liq. 2016 Sep, 221:763-767.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.9215 mL	24.6075 mL	49.2150 mL	123.0375 mL
	5 mM	0.9843 mL	4.9215 mL	9.8430 mL	24.6075 mL
	10 mM	0.4922 mL	2.4608 mL	4.9215 mL	12.3038 mL
	15 mM	0.3281 mL	1.6405 mL	3.2810 mL	8.2025 mL
	20 mM	0.2461 mL	1.2304 mL	2.4608 mL	6.1519 mL
	25 mM	0.1969 mL	0.9843 mL	1.9686 mL	4.9215 mL
	30 mM	0.1641 mL	0.8203 mL	1.6405 mL	4.1013 mL
	40 mM	0.1230 mL	0.6152 mL	1.2304 mL	3.0759 mL
	50 mM	0.0984 mL	0.4922 mL	0.9843 mL	2.4608 mL
	60 mM	0.0820 mL	0.4101 mL	0.8203 mL	2.0506 mL