4-(6-Methyl-1,2,4,5-tetrazin-3-yl)phenol

Cat. No.: HY-141274 Purity: 95.16%: FAQs
Data Sheet SDS COA Handling Instructions

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4-(6-Methyl-1,2,4,5-tetrazin-3-yl)phenol is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs.

For research use only. We do not sell to patients.

4-(6-Methyl-1,2,4,5-tetrazin-3-yl)phenol Chemical Structure

CAS No. : 58884-35-8

Size	Price	Stock	Quantity
250 mg	USD 280	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 449	In-stock	Estimated Time of Arrival: December 31
1 g		Get quote
5 g		Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

4-(6-Methyl-1,2,4,5-tetrazin-3-yl)phenol is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs^[1].

IC₅₀ & Target

Alkyl-Chain

In Vitro

PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

188.19

Formula

C₉H₈N₄O

CAS No.

58884-35-8

Appearance

Solid

Color

Pink to red

SMILES

OC1=CC=C(C2=NN=C(C)N=N2)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (531.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.3138 mL	26.5689 mL	53.1378 mL
5 mM	1.0628 mL	5.3138 mL	10.6276 mL
10 mM	0.5314 mL	2.6569 mL	5.3138 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 95.17%

Select Batch:

Data Sheet (271 KB) SDS (393 KB)

COA (247 KB) HNMR (117 KB) RP-HPLC (241 KB)

Handling Instructions (2659 KB)

References

[1]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562 [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.3138 mL	26.5689 mL	53.1378 mL	132.8445 mL
	5 mM	1.0628 mL	5.3138 mL	10.6276 mL	26.5689 mL
	10 mM	0.5314 mL	2.6569 mL	5.3138 mL	13.2844 mL
	15 mM	0.3543 mL	1.7713 mL	3.5425 mL	8.8563 mL
	20 mM	0.2657 mL	1.3284 mL	2.6569 mL	6.6422 mL
	25 mM	0.2126 mL	1.0628 mL	2.1255 mL	5.3138 mL
	30 mM	0.1771 mL	0.8856 mL	1.7713 mL	4.4281 mL
	40 mM	0.1328 mL	0.6642 mL	1.3284 mL	3.3211 mL
	50 mM	0.1063 mL	0.5314 mL	1.0628 mL	2.6569 mL
	60 mM	0.0886 mL	0.4428 mL	0.8856 mL	2.2141 mL
	80 mM	0.0664 mL	0.3321 mL	0.6642 mL	1.6606 mL
	100 mM	0.0531 mL	0.2657 mL	0.5314 mL	1.3284 mL