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  3. 4-Hydrazino-7-nitro-benzofurazan hydrazine adduct

4-Hydrazino-7-nitro-benzofurazan hydrazine adduct  (Synonyms: NBD-H)

Cat. No.: HY-W273413 Purity: 99.83%
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4-Hydrazino-7-nitro-benzofurazan hydrazine adduct is a fluorescent reagent for protein labeling.

For research use only. We do not sell to patients.

4-Hydrazino-7-nitro-benzofurazan hydrazine adduct Chemical Structure

4-Hydrazino-7-nitro-benzofurazan hydrazine adduct Chemical Structure

CAS No. : 131467-87-3

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5 mg In-stock
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Based on 1 publication(s) in Google Scholar

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Description

4-Hydrazino-7-nitro-benzofurazan hydrazine adduct is a fluorescent reagent for protein labeling.

Molecular Weight

227.18

Formula

C6H9N7O3

CAS No.
Appearance

Solid

Color

Brown to dark brown

SMILES

O=[N+](C1=CC=C(NN)C2=NON=C21)[O-].NN

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (220.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 10 mg/mL (44.02 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.4018 mL 22.0090 mL 44.0180 mL
5 mM 0.8804 mL 4.4018 mL 8.8036 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 4.4018 mL 22.0090 mL 44.0180 mL 110.0449 mL
5 mM 0.8804 mL 4.4018 mL 8.8036 mL 22.0090 mL
10 mM 0.4402 mL 2.2009 mL 4.4018 mL 11.0045 mL
15 mM 0.2935 mL 1.4673 mL 2.9345 mL 7.3363 mL
20 mM 0.2201 mL 1.1004 mL 2.2009 mL 5.5022 mL
25 mM 0.1761 mL 0.8804 mL 1.7607 mL 4.4018 mL
30 mM 0.1467 mL 0.7336 mL 1.4673 mL 3.6682 mL
40 mM 0.1100 mL 0.5502 mL 1.1004 mL 2.7511 mL
DMSO 50 mM 0.0880 mL 0.4402 mL 0.8804 mL 2.2009 mL
60 mM 0.0734 mL 0.3668 mL 0.7336 mL 1.8341 mL
80 mM 0.0550 mL 0.2751 mL 0.5502 mL 1.3756 mL
100 mM 0.0440 mL 0.2201 mL 0.4402 mL 1.1004 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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4-Hydrazino-7-nitro-benzofurazan hydrazine adduct
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