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  6. 4-Hydroxyisoleucine

4-Hydroxyisoleucine  (Synonyms: 4-​Hydroxy-​L-​isoleucine)

Cat. No.: HY-N6858 Purity: ≥98.0%
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4-Hydroxyisoleucine is an orally active amino acid that can be isolated from fenugreek seeds. 4-Hydroxyisoleucine displays insulinotropic and antidiabetic properties.

For research use only. We do not sell to patients.

4-Hydroxyisoleucine Chemical Structure

4-Hydroxyisoleucine Chemical Structure

CAS No. : 781658-23-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 USD 55 In-stock
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10 mM * 1 mL in Water USD 55 In-stock
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10 mg USD 70 In-stock
20 mg USD 110 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 4-Hydroxyisoleucine:

4-Hydroxyisoleucine Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

4-Hydroxyisoleucine is an orally active amino acid that can be isolated from fenugreek seeds. 4-Hydroxyisoleucine displays insulinotropic and antidiabetic properties[1].

In Vitro

4-Hydroxyisoleucine (20 μM, 6 h) decreases the level of iRhom2, TACE, TNF-α and MCP-1 in RAW264.7 macrophages and 3 T3-L1 adipocytes with LPS (HY-D1056) induction[2].
4-Hydroxyisoleucine (20 μM, 6 h) increases the product of M1 macrophage IL-6 and decreases the product of M2 macrophage IL-10 in the co-culture system with LPS (HY-D1056) stimulation[2].
4-Hydroxyisoleucine (0-25 μM, 16 h) increases glucose uptake and surface GLUT4myc level in L6-GLUT4myc myotubes in a concentration-dependent manner[3].
4-Hydroxyisoleucine (0-25 μM, 16 h) stimulates GLUT4myc translocation via PI-3-K/AKT-dependent mechanisms in L6-GLUT4myc myotubes[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

4-Hydroxyisoleucine Related Antibodies

Cell Viability Assay[2]

Cell Line: RAW264.7 macrophages and 3 T3-L1 adipocytes
Concentration: 0-20 μM
Incubation Time: 6 h
Result: Didn’t affect the cell viability.

Cell Migration Assay [2]

Cell Line: RAW264.7 macrophages and 3 T3-L1 adipocytes
Concentration: 20 μM
Incubation Time: 24 h
Result: Inhibited the migration of RAW264.7 macrophages in 3 T3-L1 adipocytes.

Western Blot Analysis[3]

Cell Line: L6-GLUT4myc
Concentration: 25 μM
Incubation Time: 16 h
Result: Increased the AKT (Ser-473) phosphorylation.
Didn’t effect mRNA levels of IRS-1, AKT, GSK3b, or GLUT4 in L6-GLUT4myc myotubes.
In Vivo

4-Hydroxyisoleucine (50 mg/kg, p.o., daily, 8 weeks) inhibits the increase in serum glucose in the fructose-fed rat model of metabolic syndrome[1].
4-Hydroxyisoleucine (200 mg/kg, p.o., 8 weeks) improves dyslipidemia and reduces lipid ectopic accumulation in C57BL/6 mice[4].
4-Hydroxyisoleucine (200 mg/kg, p.o., 8 weeks) decreases the expression of proinflammatory cytokine (IL-6, PAI-1, IL-1β, NF-κB, TNF-α, and MCP-1) and the proportion of proinflammatory M1 macrophages in C57BL/6 mice[4].
4-Hydroxyisoleucine (50 mg/kg, i.g., daily, 14 days) restores high levels of lipids (cholesterol, HDL, LDL and triglycerides) and uric acid in type 1 diabetic rat to that of nondiabetic controls level[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Fructose-fed rat[1]
Dosage: 50 mg/kg
Administration: Oral gavage (p.o.), daily, 8 weeks
Result: Decreased the levels of glucose and ALT.
Reduced 80% of fructosehe-induced AST release to 151 ± 45 U/mL.
Animal Model: Type 2 diabetic rat[1]
Dosage: 50 mg/kg
Administration: i.g., daily, 14 days
Result: Restored the level of HDL-cholesterol to levels comparable to controls.
Animal Model: Male C57BL/6 mice[4]
Dosage: 50-200 mg/kg
Administration: Oral gavage (p.o.), 8 weeks
Result: Decreases the body weights of mice in a dose-dependent manner.
Decreased blood glucose levels and fasting plasma insulin content in mice.
Decreased the expression of TLR4, inhibited the phosphorylation of JNK, and increased the production of IκB-α.
Animal Model: Type 1 diabetic rat[5]
Dosage: 50 mg/kg
Administration: i.g., daily, 14 days
Result: Improved appearance and heavy ocular vascularization.
Reduced the blood glucose from 500 mg/dl to 330 mg/dl.
Decreased the levels of lipid markers (TG, LDL and HDL) and uric acid.
Didn’t increase the level of inculin compared with untreated diabetic controls.
Molecular Weight

147.17

Formula

C6H13NO3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

N[C@@H]([C@@H](C)C(O)C)C(O)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (679.49 mM; Need ultrasonic)

DMSO : 1 mg/mL (6.79 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.7949 mL 33.9743 mL 67.9486 mL
5 mM 1.3590 mL 6.7949 mL 13.5897 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (679.49 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

SDS (393 KB)

COA (248 KB) HNMR (89 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 6.7949 mL 33.9743 mL 67.9486 mL 169.8716 mL
5 mM 1.3590 mL 6.7949 mL 13.5897 mL 33.9743 mL
H2O 10 mM 0.6795 mL 3.3974 mL 6.7949 mL 16.9872 mL
15 mM 0.4530 mL 2.2650 mL 4.5299 mL 11.3248 mL
20 mM 0.3397 mL 1.6987 mL 3.3974 mL 8.4936 mL
25 mM 0.2718 mL 1.3590 mL 2.7179 mL 6.7949 mL
30 mM 0.2265 mL 1.1325 mL 2.2650 mL 5.6624 mL
40 mM 0.1699 mL 0.8494 mL 1.6987 mL 4.2468 mL
50 mM 0.1359 mL 0.6795 mL 1.3590 mL 3.3974 mL
60 mM 0.1132 mL 0.5662 mL 1.1325 mL 2.8312 mL
80 mM 0.0849 mL 0.4247 mL 0.8494 mL 2.1234 mL
100 mM 0.0679 mL 0.3397 mL 0.6795 mL 1.6987 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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4-Hydroxyisoleucine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Hydroxyisoleucine781658-23-94-​Hydroxy-​L-​isoleucineOthersInsulinotropicantidiabeticorally activeL6-GLUT4myc3 T3-L1 adipocytesType 1 diabeticType 2 diabeticInhibitorinhibitorinhibit

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