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  4. 4-Nitrocatechol sulfate dipotassium salt

4-Nitrocatechol sulfate dipotassium salt  (Synonyms: Dipotassium nitrocatechol sulfate)

Cat. No.: HY-D1213 Purity: 99.43%
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4-Nitrocatechol sulfate dipotassium salt (Dipotassium nitrocatechol sulfate) is a chromogenic substrate for sulphatase.

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4-Nitrocatechol sulfate dipotassium salt Chemical Structure

4-Nitrocatechol sulfate dipotassium salt Chemical Structure

CAS No. : 14528-64-4

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Description

4-Nitrocatechol sulfate dipotassium salt (Dipotassium nitrocatechol sulfate) is a chromogenic substrate for sulphatase[1].

Molecular Weight

311.35

Formula

C6H3K2NO7S
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=[N+](C1=CC=C(O[K])C(OS(=O)(O[K])=O)=C1)[O-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (321.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 100 mg/mL (321.18 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2118 mL 16.0591 mL 32.1182 mL
5 mM 0.6424 mL 3.2118 mL 6.4236 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.43%

SDS (393 KB)

COA (251 KB) HNMR (227 KB) LCMS (95 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 3.2118 mL 16.0591 mL 32.1182 mL 80.2955 mL
5 mM 0.6424 mL 3.2118 mL 6.4236 mL 16.0591 mL
10 mM 0.3212 mL 1.6059 mL 3.2118 mL 8.0295 mL
15 mM 0.2141 mL 1.0706 mL 2.1412 mL 5.3530 mL
20 mM 0.1606 mL 0.8030 mL 1.6059 mL 4.0148 mL
25 mM 0.1285 mL 0.6424 mL 1.2847 mL 3.2118 mL
30 mM 0.1071 mL 0.5353 mL 1.0706 mL 2.6765 mL
40 mM 0.0803 mL 0.4015 mL 0.8030 mL 2.0074 mL
50 mM 0.0642 mL 0.3212 mL 0.6424 mL 1.6059 mL
60 mM 0.0535 mL 0.2677 mL 0.5353 mL 1.3383 mL
80 mM 0.0401 mL 0.2007 mL 0.4015 mL 1.0037 mL
100 mM 0.0321 mL 0.1606 mL 0.3212 mL 0.8030 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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4-Nitrocatechol sulfate dipotassium salt Related Classifications

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    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Nitrocatechol sulfate dipotassium14528-64-4Dipotassium nitrocatecholBiochemical Assay ReagentssulphataseInhibitorinhibitorinhibit

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