2. GPCR/G Protein Neuronal Signaling Vitamin D Related/Nuclear Receptor Cell Cycle/DNA Damage Membrane Transporter/Ion Channel Anti-infection Metabolic Enzyme/Protease
  3. Cannabinoid Receptor PPAR TRP Channel GPR55 Fungal Tau Protein Endogenous Metabolite
  4. Anandamide

Anandamide is an endocannabinoid. Anandamide modulates both neuronal and immune functions through two protein-coupled cannabinoid receptors (CB1 and CB2). Anandamide can activate numerous other receptors like PPARS, TRPV1, and GPR18/GPR55. Anandamide also has potential anti-fungal and anti-inflammatory activities. Anandamide can be used for the research of Alzheimer’s disease (AD) and ulcerative colitis.

For research use only. We do not sell to patients.

Anandamide Chemical Structure

Anandamide Chemical Structure

CAS No. : 94421-68-8

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Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 88 In-stock
Solution
10 mM * 1 mL in DMSO USD 88 In-stock
Liquid
5 mg USD 80 In-stock
10 mg USD 155 In-stock
25 mg USD 300 In-stock
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Based on 1 publication(s) in Google Scholar

Anandamide Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Anandamide

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TRPC TRPM TRPV TRPA TRPML

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Anandamide is an endocannabinoid. Anandamide modulates both neuronal and immune functions through two protein-coupled cannabinoid receptors (CB1 and CB2). Anandamide can activate numerous other receptors like PPARS, TRPV1, and GPR18/GPR55. Anandamide also has potential anti-fungal and anti-inflammatory activities. Anandamide can be used for the research of Alzheimer’s disease (AD) and ulcerative colitis[1][2][3][4][5].

IC50 & Target[1]

CB1

 

CB2

 

PPAR

 

TRPV1

 

Microbial Metabolite

 

Human Endogenous Metabolite

 

Cellular Effect
Cell Line Type Value Description References
RBL-2H3 IC50
> 50 μM
Compound: 14
Antiallergic activity in rat RBL2H3 cells assessed as inhibition of DNP-HSA-mediated degranulation by measuring decrease in beta-hexosaminidase activity preincubated for 30 mins followed by DNP-HSA stimulation and measured after 30 mins by 4-nitrophenyl 2
Antiallergic activity in rat RBL2H3 cells assessed as inhibition of DNP-HSA-mediated degranulation by measuring decrease in beta-hexosaminidase activity preincubated for 30 mins followed by DNP-HSA stimulation and measured after 30 mins by 4-nitrophenyl 2
[PMID: 31618024]
In Vitro

Anandamide (0-250 μg/ml, 1 h) inhibits C. albicans hyphal growth and prevents hyphal adherence to epithelial cells[4].
Anandamide (0-250 μg/ml, 2 h) alters the expression of genes involved in adhesion and hyphal morphogenesis[4].
Anandamide reduces tau phosphorylation through the inhibition of the activity of protein kinases[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anandamide Related Antibodies

RT-PCR[4]

Cell Line: HeLa cervical epithelial cells
Concentration: 0, 10, 50, 125, 250 μg/ml
Incubation Time: 2 h
Result: Repressed the expression of the HWP1 and ALS3 adhesins involved in Candida adhesion to epithelial cells and the HGC1, RAS1, EFG1 and ZAP1 regulators of hyphal morphogenesis and cell adherence. Increased the expression of NRG1, a transcriptional repressor of filamentous growth.
In Vivo

Anandamide (10 mg/kg, IP, once) considerably lowers the increase of glycemia in response to glucose ingestion compared with control, and this is associated with an improvement of glucose tolerance[2].
Anandamide (100 ng, ICV bilateral injection, single) partially prevents streptozotocin (STZ)-induced cognitive impairments, changes in synaptic markers and ventricle enlargement[3].
Anandamide exerts anti-inflammatory activities, attenuating the development of inflammation in a mouse model of ulcerative colitis[4].
Anandamide alleviates lipopolysaccharide (LPS)-induced neuroinflammation in rat primary microglial cultures[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wistar rats (3-4 months, 350-400 g, STZ-induced AD-like sporadic dementia model)[3]
Dosage: 100 ng
Administration: ICV bilateral injection, single
Result: Prevented recognition and non-associative emotional memory impairments induced by STZ, but did not influence tone fear conditioning. Decreased BAG2 (Bcl2-associated athanogene) levels, and prevented STZ-induced ventricle enlargement.
Molecular Weight

347.53

Formula

C22H37NO2
CAS No.
Appearance

Liquid (Density: 0.940±0.06 g/cm3)

Color

Colorless to light yellow

SMILES

CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(NCCO)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (287.74 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8774 mL 14.3872 mL 28.7745 mL
5 mM 0.5755 mL 2.8774 mL 5.7549 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (5.99 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (5.99 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥99.0%

SDS (416 KB)

COA (230 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8774 mL 14.3872 mL 28.7745 mL 71.9362 mL
5 mM 0.5755 mL 2.8774 mL 5.7549 mL 14.3872 mL
10 mM 0.2877 mL 1.4387 mL 2.8774 mL 7.1936 mL
15 mM 0.1918 mL 0.9591 mL 1.9183 mL 4.7957 mL
20 mM 0.1439 mL 0.7194 mL 1.4387 mL 3.5968 mL
25 mM 0.1151 mL 0.5755 mL 1.1510 mL 2.8774 mL
30 mM 0.0959 mL 0.4796 mL 0.9591 mL 2.3979 mL
40 mM 0.0719 mL 0.3597 mL 0.7194 mL 1.7984 mL
50 mM 0.0575 mL 0.2877 mL 0.5755 mL 1.4387 mL
60 mM 0.0480 mL 0.2398 mL 0.4796 mL 1.1989 mL
80 mM 0.0360 mL 0.1798 mL 0.3597 mL 0.8992 mL
100 mM 0.0288 mL 0.1439 mL 0.2877 mL 0.7194 mL
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Anandamide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anandamide94421-68-8AEACannabinoid ReceptorPPARTRP ChannelGPR55FungalTau ProteinEndogenous MetabolitePeroxisome proliferator-activated receptorsTransient receptor potential channelsG protein-coupled receptor 55StreptozotocinendocannabinoidAlzheimer’s diseaseCandida albicansHeLa cellsanti-adhesiveanti-fungalcervical epithelial cellshVSMCInhibitorinhibitorinhibit

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