5-FAM SE

Cat. No.: HY-15938 Purity: 98.64%: FAQs
Data Sheet SDS COA Handling Instructions

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5-FAM SE is a single isomer, is a fluorescent labeling reagent used for labeling peptides, proteins and nucleotides. 5-FAM SE can react with amines and can yield stable amine conjugates.

For research use only. We do not sell to patients.

5-FAM SE Chemical Structure

CAS No. : 92557-80-7

Size	Price	Stock	Quantity
5 mg	USD 66	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 105	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 315	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 500	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Purity & Documentation
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Description

5-FAM SE is a single isomer, is a fluorescent labeling reagent used for labeling peptides, proteins and nucleotides. 5-FAM SE can react with amines and can yield stable amine conjugates^[1]^[2].

In Vitro

The capillary electrophoresis (CE)-laser-induced fluorescent (LIF) method with 5-FAM SE (5-Carboxyfluorescein N-succinimidyl ester) derivatization for the analysis of serotonin can be used to quantify luminally released 5-HT with high sensitivity. CFSE is an excellent dye for the derivatization of 5-HT^[1].
5-FAM SE (5-Carboxyfluorescein succinimidyl ester) is chosen as the labeling reagent to precapillary derivatize the two marker aminohydroxyphenylalanine (AHP) isomers produced after reductive hydrolysis of pheomelanin with hydriodic acid (HI)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

473.39

Formula

C₂₅H₁₅NO₉

CAS No.

92557-80-7

Appearance

Solid

Color

Light yellow to yellow

Emission (Em)

515

Excitation (Ex)

488

SMILES

O=C(C1=CC2=C(C3(C4=C(OC5=C3C=CC(O)=C5)C=C(O)C=C4)OC2=O)C=C1)ON6C(CCC6=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (52.81 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1124 mL	10.5621 mL	21.1242 mL
5 mM	0.4225 mL	2.1124 mL	4.2248 mL
10 mM	0.2112 mL	1.0562 mL	2.1124 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.64%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (259 KB) HNMR (271 KB) LCMS (270 KB)

Handling Instructions (2659 KB)

References

[1]. Sheng-da Qi, et al. Quantification of luminally released serotonin in rat proximal colon by capillary electrophoresis with laser-induced fluorescence detection. Anal Bioanal Chem. 2009 Apr;393(8):2059-66. [Content Brief]

[2]. Xiao-Ling Zhang, et al. Sensitive determination of pheomelanin after 5-carboxyfluorescein succinimidyl ester precapillary derivatization and micellar electrokinetic capillary chromatography with laser-induced fluorescence detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jan 1;861(1):136-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1124 mL	10.5621 mL	21.1242 mL	52.8106 mL
	5 mM	0.4225 mL	2.1124 mL	4.2248 mL	10.5621 mL
	10 mM	0.2112 mL	1.0562 mL	2.1124 mL	5.2811 mL
	15 mM	0.1408 mL	0.7041 mL	1.4083 mL	3.5207 mL
	20 mM	0.1056 mL	0.5281 mL	1.0562 mL	2.6405 mL
	25 mM	0.0845 mL	0.4225 mL	0.8450 mL	2.1124 mL
	30 mM	0.0704 mL	0.3521 mL	0.7041 mL	1.7604 mL
	40 mM	0.0528 mL	0.2641 mL	0.5281 mL	1.3203 mL
	50 mM	0.0422 mL	0.2112 mL	0.4225 mL	1.0562 mL

5-FAM SE Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-FAM SE92557-80-7Fluorescent DyeSerotoninfluorescentlabelingpeptidesproteinsnucleotidesamineInhibitorinhibitorinhibit

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