5-Hydroxymethyl-2'-deoxyuridine

Name: 5-Hydroxymethyl-2'-deoxyuridine
Brand: MedChemExpress
SKU: HY-129983
Rating: 4.5 (1 reviews)

Cat. No.: HY-129983 Purity: 99.98%: FAQs
Data Sheet SDS COA Handling Instructions

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5-Hydroxymethyl-2'-deoxyuridine is a nucleoside analog. 5-Hydroxymethyl-2'-deoxyuridine inhibits the replication of multiple human leukemia cell lines with IC₅₀ values of 1.7-5.8 μM. 5-Hydroxymethyl-2'-deoxyuridine prolongs the survival of mice carrying L1210 leukemia. 5-Hydroxymethyl-2'-deoxyuridine can be used for the research of cell replication and leukemia.

For research use only. We do not sell to patients.

5-Hydroxymethyl-2'-deoxyuridine Chemical Structure

CAS No. : 5116-24-5

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Free Sample (0.1 - 0.5 mg)		Apply Now
25 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 140	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
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500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
CEM-SS	CC₅₀	120 μM Compound: 1c	Cytotoxicity against human CEM-SS cells by MTT assay Cytotoxicity against human CEM-SS cells by MTT assay	[PMID: 20112915]
CEM-SS	IC₅₀	30 μM Compound: 1c	Antiviral activity against HIV1 LAI in human CEM-SS cells assessed as RT activity after 5 days by single passage assay Antiviral activity against HIV1 LAI in human CEM-SS cells assessed as RT activity after 5 days by single passage assay	[PMID: 20112915]
L5178Y	IC₅₀	8.6 μM Compound: HmdUrd	In vitro growth inhibition of L5178Y-Parental murine leukemia cells by incorporation of [14C]-Thd. In vitro growth inhibition of L5178Y-Parental murine leukemia cells by incorporation of [14C]-Thd.	[PMID: 11101356]
MT4	IC₅₀	> 130 μM Compound: 1c	Antiviral activity against HIV1 3B in human MT4 cells assessed as RT activity after 5 days by single passage assay Antiviral activity against HIV1 3B in human MT4 cells assessed as RT activity after 5 days by single passage assay	[PMID: 20112915]
MT4	CC₅₀	130 μM Compound: 1c	Cytotoxicity against human MT4 cells by MTT assay Cytotoxicity against human MT4 cells by MTT assay	[PMID: 20112915]

In Vitro

5-Hydroxymethyl-2'-deoxyuridine (0-10 μM) inhibits the replication of Sarcoma 180 cells and Ehrlich ascites carcinoma cells with ED₅₀ values of 8.5 and 4 μM, respectively^[1].
5-Hydroxymethyl-2'-deoxyuridine inhibits herpes simplex virus type 1 (HSV-1) pyrimidine 2’-deoxyribonucleoside kinase with a K_i value of 3.5 μM^[1].
5-Hydroxymethyl-2'-deoxyuridine inhibits the replication of multiple human leukemia cell lines with IC₅₀ values of 1.7-5.8 μM^[2].
5-Hydroxymethyl-2'-deoxyuridine (10-100 μM) shows dose-dependent toxicity against a human acute promyelocytic leukemia cell line^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

5-Hydroxymethyl-2'-deoxyuridine (0, 5 and 50 mg/kg; i.p.; once) increases the survival of DBA/2 mice carrying L1210 leukemia^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

258.23

Formula

C₁₀H₁₄N₂O₆

CAS No.

5116-24-5

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1C[C@H](N2C(NC(C(CO)=C2)=O)=O)O[C@@H]1CO

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 116.67 mg/mL (451.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8725 mL	19.3626 mL	38.7252 mL
5 mM	0.7745 mL	3.8725 mL	7.7450 mL
10 mM	0.3873 mL	1.9363 mL	3.8725 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.98%

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Data Sheet (276 KB) SDS (393 KB)

COA (254 KB) HNMR (247 KB) RP-HPLC (247 KB) MS (177 KB)

Handling Instructions (2659 KB)

References

[1]. Shiau GT, et al. Synthesis and biological activities of 5-(hydroxymethyl, azidomethyl, or aminomethyl)-2'-deoxyuridine and related 5'-substituted analogues. J Med Chem. 1980 Feb;23(2):127-33. [Content Brief]

[2]. Kahilainen LI, et al. 5-Hydroxymethyl-2'-deoxyuridine. Cytotoxicity and DNA incorporation studied by using a novel [2-14C]-derivative with normal and leukemic human hematopoietic cells. Acta Chem Scand B. 1985;39(6):477-84. [Content Brief]

[3]. JA Vilpo, et al. 5-Hydroxymethyl-2′-Deoxyuridine: Studies of Antileukemic Properties in Vitro and in Vivo. Nucleosides and Nucleotides . 1987.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.8725 mL	19.3626 mL	38.7252 mL	96.8129 mL
	5 mM	0.7745 mL	3.8725 mL	7.7450 mL	19.3626 mL
	10 mM	0.3873 mL	1.9363 mL	3.8725 mL	9.6813 mL
	15 mM	0.2582 mL	1.2908 mL	2.5817 mL	6.4542 mL
	20 mM	0.1936 mL	0.9681 mL	1.9363 mL	4.8406 mL
	25 mM	0.1549 mL	0.7745 mL	1.5490 mL	3.8725 mL
	30 mM	0.1291 mL	0.6454 mL	1.2908 mL	3.2271 mL
	40 mM	0.0968 mL	0.4841 mL	0.9681 mL	2.4203 mL
	50 mM	0.0775 mL	0.3873 mL	0.7745 mL	1.9363 mL
	60 mM	0.0645 mL	0.3227 mL	0.6454 mL	1.6135 mL
	80 mM	0.0484 mL	0.2420 mL	0.4841 mL	1.2102 mL
	100 mM	0.0387 mL	0.1936 mL	0.3873 mL	0.9681 mL