2. Metabolic Enzyme/Protease
  3. Glutathione S-transferase
  4. 7-Acetoxy-4-methylcoumarin

7-Acetoxy-4-methylcoumarin  (Synonyms: 4-Methylumbelliferyl acetate)

Cat. No.: HY-137877 Purity: 98.65%
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7-Acetoxy-4-methylcoumarin is an inhibitor of GST.7-Acetoxy-4-methylcoumarin inhibits AFB1-DNA binding in vitro with 36.7% inhibition.

For research use only. We do not sell to patients.

7-Acetoxy-4-methylcoumarin Chemical Structure

7-Acetoxy-4-methylcoumarin Chemical Structure

CAS No. : 2747-05-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 27 In-stock
Solution
10 mM * 1 mL in DMSO USD 27 In-stock
Solid
100 mg USD 25 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

7-Acetoxy-4-methylcoumarin Related Antibodies

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Description

7-Acetoxy-4-methylcoumarin is an inhibitor of GST.7-Acetoxy-4-methylcoumarin inhibits AFB1-DNA binding in vitro with 36.7% inhibition[1][2].

Molecular Weight

218.21

Formula

C12H10O4
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=C1C=C(C)C2=CC=C(OC(C)=O)C=C2O1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (114.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.5827 mL 22.9137 mL 45.8274 mL
5 mM 0.9165 mL 4.5827 mL 9.1655 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (11.46 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.5827 mL 22.9137 mL 45.8274 mL 114.5685 mL
5 mM 0.9165 mL 4.5827 mL 9.1655 mL 22.9137 mL
10 mM 0.4583 mL 2.2914 mL 4.5827 mL 11.4569 mL
15 mM 0.3055 mL 1.5276 mL 3.0552 mL 7.6379 mL
20 mM 0.2291 mL 1.1457 mL 2.2914 mL 5.7284 mL
25 mM 0.1833 mL 0.9165 mL 1.8331 mL 4.5827 mL
30 mM 0.1528 mL 0.7638 mL 1.5276 mL 3.8190 mL
40 mM 0.1146 mL 0.5728 mL 1.1457 mL 2.8642 mL
50 mM 0.0917 mL 0.4583 mL 0.9165 mL 2.2914 mL
60 mM 0.0764 mL 0.3819 mL 0.7638 mL 1.9095 mL
80 mM 0.0573 mL 0.2864 mL 0.5728 mL 1.4321 mL
100 mM 0.0458 mL 0.2291 mL 0.4583 mL 1.1457 mL
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7-Acetoxy-4-methylcoumarin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

7-Acetoxy-4-methylcoumarin2747-05-94-MethylumbelliferylGlutathione S-transferaseGlutathione transferasesGSTsCoumarin7-AMCTransacetylaseGlutathione -S-transferaseGSTInhibitorinhibitorinhibit

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