7-Amino-4-methylcoumarin-3-acetic acid

Cat. No.: HY-D0025 Purity: 99.89%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

7-Amino-4-methylcoumarin-3-acetic acid is a fluorescent protein labelling agent. 7-Amino-4-methylcoumarin-3-acetic acid emits in the blue region (440-460 nm) on activation with UV light (350 nm).

For research use only. We do not sell to patients.

7-Amino-4-methylcoumarin-3-acetic acid Chemical Structure

CAS No. : 106562-32-7

Size	Price	Stock	Quantity
25 mg	USD 35	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

7-Amino-4-methylcoumarin-3-acetic acid is a fluorescent protein labelling agent. 7-Amino-4-methylcoumarin-3-acetic acid emits in the blue region (440-460 nm) on activation with UV light (350 nm)^[1].

Molecular Weight

233.22

Formula

C₁₂H₁₁NO₄

CAS No.

106562-32-7

Appearance

Solid

Color

Off-white to light brown

Emission (Em)

454

Excitation (Ex)

344

SMILES

O=C(O)CC1=C(C)C2=CC=C(N)C=C2OC1=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (535.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.2878 mL	21.4390 mL	42.8780 mL
5 mM	0.8576 mL	4.2878 mL	8.5756 mL
10 mM	0.4288 mL	2.1439 mL	4.2878 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.89%

Select Batch:

Data Sheet (267 KB) SDS (393 KB)

COA (246 KB) HNMR (151 KB) LCMS (265 KB)

Handling Instructions (2659 KB)

References

[1]. Khalfan H, et al. Aminomethyl coumarin acetic acid: a new fluorescent labelling agent for proteins. Histochem J. 1986;18(9):497-499. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.2878 mL	21.4390 mL	42.8780 mL	107.1949 mL
	5 mM	0.8576 mL	4.2878 mL	8.5756 mL	21.4390 mL
	10 mM	0.4288 mL	2.1439 mL	4.2878 mL	10.7195 mL
	15 mM	0.2859 mL	1.4293 mL	2.8585 mL	7.1463 mL
	20 mM	0.2144 mL	1.0719 mL	2.1439 mL	5.3597 mL
	25 mM	0.1715 mL	0.8576 mL	1.7151 mL	4.2878 mL
	30 mM	0.1429 mL	0.7146 mL	1.4293 mL	3.5732 mL
	40 mM	0.1072 mL	0.5360 mL	1.0719 mL	2.6799 mL
	50 mM	0.0858 mL	0.4288 mL	0.8576 mL	2.1439 mL
	60 mM	0.0715 mL	0.3573 mL	0.7146 mL	1.7866 mL
	80 mM	0.0536 mL	0.2680 mL	0.5360 mL	1.3399 mL
	100 mM	0.0429 mL	0.2144 mL	0.4288 mL	1.0719 mL