8-Bromo-cAMP (Synonyms: 8-Br-Camp)

Name: 8-Bromo-cAMP
Brand: MedChemExpress
SKU: HY-12306A
Rating: 5.0 (22 reviews)

Cat. No.: HY-12306A Purity: 99.41%: FAQs
Data Sheet SDS COA Handling Instructions

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8-Bromo-cAMP (8-Br-Camp), a cyclic AMP analog, is an activator of cyclic AMP-dependent protein kinase (PKA). 8-Bromo-cAMP has anti-proliferative and apoptotic effects against cancer cells.

For research use only. We do not sell to patients.

8-Bromo-cAMP Chemical Structure

CAS No. : 23583-48-4

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5 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
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Based on 22 publication(s) in Google Scholar

Other Forms of 8-Bromo-cAMP:

8-Bromo-cAMP sodium salt In-stock

22 Publications Citing Use of MCE 8-Bromo-cAMP

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Description

8-Bromo-cAMP (8-Br-Camp), a cyclic AMP analog, is an activator of cyclic AMP-dependent protein kinase (PKA). 8-Bromo-cAMP has anti-proliferative and apoptotic effects against cancer cells^[1]^[2].

IC₅₀ & Target

PKA^[1]

In Vitro

8-Bromo-cAMP (0.1/0.5 mM) enhances the reprogramming efficiency of human neonatal foreskin fibroblast (HFF1) cells, and 0.1 mM of 8-Bromo-cAMP shows a synergistic effect with Valproic acid (HY-10585) (0.5 mM)^[1].
8-Bromo-cAMP (20 μM, 24 and 48 h) induces apoptosis in esophageal cancer cell line (Eca-109)^[2].
8-Bromo-cAMP (0.5 mM, 2 days) induces decidualization of human endometrial stromal cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

8-Bromo-cAMP (60 mg/kg/day, i.p., 7 days) reduces tumor in CT26 tumor mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	CT26 tumor mice^[4]
Dosage:	60 mg/kg/day
Administration:	i.p., 7 days
Result:	Decreases amounts of primary CRC tumor nodules and liver metastases. Reduces vasculogenic mimicry (PAS–CD31 staining of colorectal and intestinal tumors). Inhibits cAMP and VEGF expression, increases expression of PKA in tumor tissues.

Molecular Weight

408.10

Formula

C₁₀H₁₁BrN₅O₆P

CAS No.

23583-48-4

Appearance

Solid

Color

Off-white to light yellow

SMILES

OP1(O[C@@]2([H])[C@@H](O)[C@@H](O[C@]2([H])CO1)N3C(Br)=NC4=C3N=CN=C4N)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 5.74 mg/mL (14.07 mM; ultrasonic and warming and adjust pH to 9 with 1 M NaOH and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4504 mL	12.2519 mL	24.5038 mL
5 mM	0.4901 mL	2.4504 mL	4.9008 mL
10 mM	0.2450 mL	1.2252 mL	2.4504 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.41%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (249 KB) HNMR (262 KB) CNMR (257 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Wang Y, et al. A cyclic AMP analog, 8-Br-cAMP, enhances the induction of pluripotency in human fibroblast cells. Stem Cell Rev. 2011 Jun;7(2):331-41. [Content Brief]

[2]. Wang HM, et al. Dual effects of 8-Br-cAMP on differentiation and apoptosis of human esophageal cancer cell line Eca-109. World J Gastroenterol. 2005 Nov 7;11(41):6538-42. [Content Brief]

[3]. Baek MO, et al. Differential regulation of mTORC1 and mTORC2 is critical for 8-Br-cAMP-induced decidualization. Exp Mol Med. 2018 Oct 30;50(10):1-11. [Content Brief]

[4]. Wang S, et al. Angiogenesis and vasculogenic mimicry are inhibited by 8-Br-cAMP through activation of the cAMP/PKA pathway in colorectal cancer. Onco Targets Ther. 2018 Jul 2;11:3765-3774 [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.4504 mL	12.2519 mL	24.5038 mL	61.2595 mL
	5 mM	0.4901 mL	2.4504 mL	4.9008 mL	12.2519 mL
	10 mM	0.2450 mL	1.2252 mL	2.4504 mL	6.1259 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

8-Bromo-cAMP Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

8-Bromo-cAMP23583-48-48-Br-CampPKAApoptosisProtein kinase AHFF1Eca-109endometrial stromal cellInhibitorinhibitorinhibit

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8-Bromo-cAMP (Synonyms: 8-Br-Camp)

Customer Review

Other Forms of 8-Bromo-cAMP:

8-Bromo-cAMP Related Antibodies

22 Publications Citing Use of MCE 8-Bromo-cAMP

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All PKA Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

8-Bromo-cAMP Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

8-Bromo-cAMP Related Classifications

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