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  3. 8-Bromo-cAMP sodium salt

8-Bromo-cAMP sodium salt  (Synonyms: 8-Br-Camp sodium salt)

Cat. No.: HY-12306 Purity: 99.91%
SDS COA Handling Instructions

8-Bromo-cAMP sodium (8-Br-Camp) sodium salt, a cyclic AMP analog, is an activator of cyclic AMP-dependent protein kinase (PKA). 8-Bromo-cAMP sodium salt has anti-proliferative and apoptotic effects against cancer cells.

For research use only. We do not sell to patients.

8-Bromo-cAMP sodium salt Chemical Structure

8-Bromo-cAMP sodium salt Chemical Structure

CAS No. : 76939-46-3

Size Price Stock Quantity
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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Solid
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10 mg USD 70 In-stock
25 mg USD 120 In-stock
50 mg USD 185 In-stock
100 mg USD 280 In-stock
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Customer Review

Based on 22 publication(s) in Google Scholar

Other Forms of 8-Bromo-cAMP sodium salt:

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

8-Bromo-cAMP sodium (8-Br-Camp) sodium salt, a cyclic AMP analog, is an activator of cyclic AMP-dependent protein kinase (PKA). 8-Bromo-cAMP sodium salt has anti-proliferative and apoptotic effects against cancer cells[1][2].

IC50 & Target

PKA[1]

In Vitro

8-Bromo-cAMP (0.1/0.5 mM) sodium salt enhances the reprogramming efficiency of human neonatal foreskin fibroblast (HFF1) cells, and 0.1 mM of 8-Bromo-cAMP shows a synergistic effect with Valproic acid (HY-10585) (0.5 mM)[1].
8-Bromo-cAMP (20 μM, 24 and 48 h) sodium salt induces apoptosis in esophageal cancer cell line (Eca-109)[2].
8-Bromo-cAMP (0.5 mM, 2 days) sodium salt induces decidualization of human endometrial stromal cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

8-Bromo-cAMP (60 mg/kg/day, i.p., 7 days) sodium salt reduces tumor in CT26 tumor mice[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: CT26 tumor mice[4]
Dosage: 60 mg/kg/day
Administration: i.p., 7 days
Result: Decreases amounts of primary CRC tumor nodules and liver metastases.
Reduces vasculogenic mimicry (PAS–CD31 staining of colorectal and intestinal tumors).
Inhibits cAMP and VEGF expression, increases expression of PKA in tumor tissues.
Molecular Weight

430.08

Formula

C10H10BrN5NaO6P

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

BrC1=NC2=C(N=CN=C2N)N1[C@H](O[C@@](CO3)([H])[C@@]4([H])OP3(O[Na])=O)[C@@H]4O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 125 mg/mL (290.64 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 100 mg/mL (232.51 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3251 mL 11.6257 mL 23.2515 mL
5 mM 0.4650 mL 2.3251 mL 4.6503 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.84 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (4.84 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (232.51 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.91%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.3251 mL 11.6257 mL 23.2515 mL 58.1287 mL
5 mM 0.4650 mL 2.3251 mL 4.6503 mL 11.6257 mL
10 mM 0.2325 mL 1.1626 mL 2.3251 mL 5.8129 mL
15 mM 0.1550 mL 0.7750 mL 1.5501 mL 3.8752 mL
20 mM 0.1163 mL 0.5813 mL 1.1626 mL 2.9064 mL
25 mM 0.0930 mL 0.4650 mL 0.9301 mL 2.3251 mL
30 mM 0.0775 mL 0.3875 mL 0.7750 mL 1.9376 mL
40 mM 0.0581 mL 0.2906 mL 0.5813 mL 1.4532 mL
50 mM 0.0465 mL 0.2325 mL 0.4650 mL 1.1626 mL
60 mM 0.0388 mL 0.1938 mL 0.3875 mL 0.9688 mL
80 mM 0.0291 mL 0.1453 mL 0.2906 mL 0.7266 mL
100 mM 0.0233 mL 0.1163 mL 0.2325 mL 0.5813 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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8-Bromo-cAMP sodium salt
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