8-pCPT-2'-O-Me-cAMP-AM

Name: 8-pCPT-2'-O-Me-cAMP-AM
Brand: MedChemExpress
SKU: HY-107544
Rating: 4.5 (1 reviews)

Cat. No.: HY-107544 Purity: ≥99.0%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

8-pCPT-2'-O-Me-cAMP-AM is a cyclic AMP analogue, selectively activates Epac-Rap signaling pathway. 8-pCPT-2'-O-Me-cAMP-AM protects renal function by activating Epac from ischemia injury. 8-pCPT-2'-O-Me-cAMP-AM also stimulates insulin secretion by interaction with PKA pathway.

For research use only. We do not sell to patients.

8-pCPT-2'-O-Me-cAMP-AM Chemical Structure

CAS No. : 1152197-23-3

Size	Price	Stock	Quantity
100 μg		In-stock	Estimated Time of Arrival: December 31

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Based on 1 publication(s) in Google Scholar

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Description

In Vitro

8-pCPT-2'-O-Me-cAMP-AM (2.5 μM; 30 min) activates Epac and prevents adherens junction disassembly during hypoxia (60 min)^[1].
8-pCPT-2'-O-Me-cAMP-AM can cross the plasma membrane and is able to alter diverse cellular functions that include Rap1 GTPase activity, PKB, and ERK1/2 protein kinase activity, phospholipase C activity, Ca²⁺ signaling, ion channel activity, exocytosis, cell adhesion, and gene expression^[2].
8-pCPT-2'-O-Me-cAMP-AM stimulates insulin secretion with dose-dependent and glucose metabolism-dependent (0.1 or 1.11 mM) actions^[2].
8-pCPT-2'-O-Me-cAMP-AM (20 μM) activates the cAMP reporter Epac1-camps, while 8-pCPT-2'-O-Me-cAMP doesn’t in INS-1 cells^[2].
8-pCPT-2'-O-Me-cAMP-AM (0.3-3.0 μM; 0-300 sec) activates Epac1-camps in a dose- and time-dependent manner in high throughput assay^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

8-pCPT-2'-O-Me-cAMP-AM (intrarenal injection; ) activates renal Rap1 and likely is caused by activation of Epac in the tubular epithelium^[1].
8-pCPT-2'-O-Me-cAMP-AM preserves renal function by Epac activation and reduces tubular epithelial-cell stress during ischemia^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	IR injuried mouse model^[1]
Dosage:	1.45 mM
Administration:	Intrarenal injection; mice were sacrificed at 24, 48, or 72 hours after ischemia
Result:	Protected renal injury during ischemia.

Molecular Weight

557.90

Formula

C₂₀H₂₁ClN₅O₈PS

CAS No.

1152197-23-3

Appearance

Oil

Color

Colorless to light yellow

SMILES

NC1=C2C(N([C@@]3([H])[C@@H]([C@@]4([H])[C@](COP(OCOC(C)=O)(O4)=O)([H])O3)OC)C(SC5=CC=C(C=C5)Cl)=N2)=NC=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 55.79 mg/mL (100.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7924 mL	8.9622 mL	17.9244 mL
5 mM	0.3585 mL	1.7924 mL	3.5849 mL
10 mM	0.1792 mL	0.8962 mL	1.7924 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation

Purity: ≥99.0%

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Data Sheet (279 KB) SDS (252 KB)

COA (257 KB)

Handling Instructions (2659 KB)

References

[1]. Stokman G, et al. Epac-Rap signaling reduces cellular stress and ischemia-induced kidney failure. J Am Soc Nephrol. 2011 May;22(5):859-72. [Content Brief]

[2]. Chepurny OG, et al. Enhanced Rap1 activation and insulin secretagogue properties of an acetoxymethyl ester of an Epac-selective cyclic AMP analog in rat INS-1 cells: studies with 8-pCPT-2'-O-Me-cAMP-AM. J Biol Chem. 2009 Apr 17;284(16):10728-36. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7924 mL	8.9622 mL	17.9244 mL	44.8109 mL
	5 mM	0.3585 mL	1.7924 mL	3.5849 mL	8.9622 mL
	10 mM	0.1792 mL	0.8962 mL	1.7924 mL	4.4811 mL
	15 mM	0.1195 mL	0.5975 mL	1.1950 mL	2.9874 mL
	20 mM	0.0896 mL	0.4481 mL	0.8962 mL	2.2405 mL
	25 mM	0.0717 mL	0.3585 mL	0.7170 mL	1.7924 mL
	30 mM	0.0597 mL	0.2987 mL	0.5975 mL	1.4937 mL
	40 mM	0.0448 mL	0.2241 mL	0.4481 mL	1.1203 mL
	50 mM	0.0358 mL	0.1792 mL	0.3585 mL	0.8962 mL
	60 mM	0.0299 mL	0.1494 mL	0.2987 mL	0.7468 mL
	80 mM	0.0224 mL	0.1120 mL	0.2241 mL	0.5601 mL