2. Anti-infection
  3. Bacterial Antibiotic
  4. Apramycin sulfate

Apramycin sulfate  (Synonyms: Nebramycin II sulfate)

Cat. No.: HY-B1329 Purity: ≥98.0%
COA Handling Instructions

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Apramycin (EBL 1003) is an orally active, acidic pH tolerant and aminoglycoside-modifying-enzymes-tolerant aminoglycoside antibiotic which inhibits protein biosynthesis by targeting the bacterial ribosome. Apramycin is a potential anti-drug-resistance antibiotic.

For research use only. We do not sell to patients.

Apramycin sulfate Chemical Structure

Apramycin sulfate Chemical Structure

CAS No. : 65710-07-8

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 USD 33 In-stock
Solution
10 mM * 1 mL in Water USD 33 In-stock
Solid
100 mg USD 30 In-stock
500 mg USD 60 In-stock
1 g USD 80 In-stock
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Based on 3 publication(s) in Google Scholar

Apramycin sulfate Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

Apramycin (EBL 1003) is an orally active, acidic pH tolerant and aminoglycoside-modifying-enzymes-tolerant aminoglycoside antibiotic which inhibits protein biosynthesis by targeting the bacterial ribosome. Apramycin is a potential anti-drug-resistance antibiotic[1][2][3].

IC50 & Target

Aminoglycoside

 

In Vitro

Apramycin (4 mg/L) inhibits 99% of K. pneumoniae and 93% of Enterobacter clinical isolates. Apramycin (8 mg/L) inhibits 99% of all E. coli, 98% of C. freundii, 96% of Providencia spp., 92% of S. marcescens, 97% of M. morganii and 100% of P. mirabilis clinical isolates, exhibiting significantly better antimicrobial activity than the clinical standard-of-care aminoglycosides Gentamicin and Amikacin[1].
Apramycin (100 μM, 10 min) exerts an 11% decrease in cytoplasmic uptake of E.coli when lowering the pH from 7.3 to 5.7 while the amounts of Gentamicin and Amikacin are reduced by 62% and 51%, respectively[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apramycin sulfate Related Antibodies
In Vivo

Apramycin (200 mg/kg/d, s.c., 9 d) demonstrates significant in vivo efficacy in lungs of M. tuberculosis low-dose aerosol infection model IFN-γ knockout mice[2].
Apramycin (16, 32, 80 mg/kg, s.c., 24 h) dose-dependently reduces bacterial burden in the kidneys between 2-5 log10 and in blood between 2-3 log10 for neutropenic model of Staphylococcus aureus septicemia mice[2].
Apramycin (50 mg/kg/d, s.c., 21 d) induces nephrotoxicity scores comparable to those induced by Gentamicin (10 mg/kg/d) [3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: M. tuberculosis low-dose aerosol infection model of IFN-γ knockout mice [2]
Dosage: 200 mg/kg/d
Administration: Subcutaneousinjection (s.c.) for 9 d
Result: Showed a 2.4-log10 CFU reduction and better antituberculous activity compared to Amikacin (1.8-log10 reduction).
Clinical Trial
Molecular Weight

637.66

Formula

C21H43N5O15S
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

O[C@H]1[C@](O[C@H](O[C@@]([C@H](C[C@H]2N)N)([H])[C@@H]([C@H]2O)O)[C@H](N)C3)([H])[C@@]3([H])O[C@H](O[C@@]([C@@H]([C@@H](O)[C@@H]4N)O)([H])O[C@@H]4CO)[C@H]1NC.O=S(O)(O)=O
Structure Classification
Initial Source

Streptoalloteichus tenebrarius
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : ≥ 200 mg/mL (313.65 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5682 mL 7.8412 mL 15.6823 mL
5 mM 0.3136 mL 1.5682 mL 3.1365 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (156.82 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

COA (259 KB) HNMR (274 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.5682 mL 7.8412 mL 15.6823 mL 39.2058 mL
5 mM 0.3136 mL 1.5682 mL 3.1365 mL 7.8412 mL
10 mM 0.1568 mL 0.7841 mL 1.5682 mL 3.9206 mL
15 mM 0.1045 mL 0.5227 mL 1.0455 mL 2.6137 mL
20 mM 0.0784 mL 0.3921 mL 0.7841 mL 1.9603 mL
25 mM 0.0627 mL 0.3136 mL 0.6273 mL 1.5682 mL
30 mM 0.0523 mL 0.2614 mL 0.5227 mL 1.3069 mL
40 mM 0.0392 mL 0.1960 mL 0.3921 mL 0.9801 mL
50 mM 0.0314 mL 0.1568 mL 0.3136 mL 0.7841 mL
60 mM 0.0261 mL 0.1307 mL 0.2614 mL 0.6534 mL
80 mM 0.0196 mL 0.0980 mL 0.1960 mL 0.4901 mL
100 mM 0.0157 mL 0.0784 mL 0.1568 mL 0.3921 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Apramycin sulfate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Apramycin65710-07-8Nebramycin IIBacterialAntibioticAminoglycosideantibioticprotein biosynthesisbacterial ribosomeaminoglycoside-modifying enzymesacidic pH toleranceanti-drug-resistanceInhibitorinhibitorinhibit

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