Auristatin E

Name: Auristatin E
Brand: MedChemExpress
SKU: HY-15582
Rating: 4.5 (1 reviews)

Cat. No.: HY-15582 Purity: 99.82%: FAQs
Data Sheet SDS COA Handling Instructions

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Auristatin E is a cytotoxic microtubule polymerization inhibitor with potent and selective antitumor activity. Auristatin E is a cytotoxin in antibody-drug conjugates (ADC). Auristatin E inhibits cell division by blocking the polymerisation of tubulin, promising for research in B-cell malignancies. Auristatin E, a synthetic analogue of the Dolastatin 10 (HY-15580), is linear peptides comprised of four amino acids.

For research use only. We do not sell to patients.

Auristatin E Chemical Structure

CAS No. : 160800-57-7

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5 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 270	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 400	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Auristatin E (GMP) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Auristatin

Cellular Effect

Cell Line	Type	Value	Description	References
BXPC-3	GI₅₀	> 1.37 nM Compound: 2a	Growth inhibition of human BxPC3 cells incubated for 48 hrs by MTT assay Growth inhibition of human BxPC3 cells incubated for 48 hrs by MTT assay	[PMID: 28211277]
BXPC-3	GI₅₀	> 1.37 nM Compound: Auristatin E	Growth inhibition of human BxPC3 cells after 48 hrs by SRB assay Growth inhibition of human BxPC3 cells after 48 hrs by SRB assay	[PMID: 28926240]
BXPC-3	GI₅₀	> 1.37 nM Compound: Auristatin E	Growth inhibition of human BxPC3 cells after 48 hrs by SRB assay Growth inhibition of human BxPC3 cells after 48 hrs by SRB assay	[PMID: 28895394]
DU-145	GI₅₀	0.424 nM Compound: 2a	Growth inhibition of human DU145 cells incubated for 48 hrs by MTT assay Growth inhibition of human DU145 cells incubated for 48 hrs by MTT assay	[PMID: 28211277]
DU-145	GI₅₀	0.424 nM Compound: Auristatin E	Growth inhibition of human DU145 cells after 48 hrs by SRB assay Growth inhibition of human DU145 cells after 48 hrs by SRB assay	[PMID: 28926240]
DU-145	GI₅₀	0.424 nM Compound: Auristatin E	Growth inhibition of human DU145 cells after 48 hrs by SRB assay Growth inhibition of human DU145 cells after 48 hrs by SRB assay	[PMID: 28895394]
KM-20L2	GI₅₀	0.492 nM Compound: 2a	Growth inhibition of human KM20L2 cells incubated for 48 hrs by MTT assay Growth inhibition of human KM20L2 cells incubated for 48 hrs by MTT assay	[PMID: 28211277]
KM-20L2	GI₅₀	0.492 nM Compound: Auristatin E	Growth inhibition of human KM20L2 cells after 48 hrs by SRB assay Growth inhibition of human KM20L2 cells after 48 hrs by SRB assay	[PMID: 28926240]
KM-20L2	GI₅₀	0.492 nM Compound: Auristatin E	Growth inhibition of human KM20L2 cells after 48 hrs by SRB assay Growth inhibition of human KM20L2 cells after 48 hrs by SRB assay	[PMID: 28895394]
MCF7	GI₅₀	0.232 nM Compound: 2a	Growth inhibition of human MCF7 cells incubated for 48 hrs by MTT assay Growth inhibition of human MCF7 cells incubated for 48 hrs by MTT assay	[PMID: 28211277]
MCF7	GI₅₀	0.232 nM Compound: Auristatin E	Growth inhibition of human MCF7 cells after 48 hrs by SRB assay Growth inhibition of human MCF7 cells after 48 hrs by SRB assay	[PMID: 28926240]
MCF7	GI₅₀	0.232 nM Compound: Auristatin E	Growth inhibition of human MCF7 cells after 48 hrs by SRB assay Growth inhibition of human MCF7 cells after 48 hrs by SRB assay	[PMID: 28895394]
NCI-H460	GI₅₀	0.533 nM Compound: 2a	Growth inhibition of human NCI-H460 cells incubated for 48 hrs by MTT assay Growth inhibition of human NCI-H460 cells incubated for 48 hrs by MTT assay	[PMID: 28211277]
NCI-H460	GI₅₀	0.533 nM Compound: Auristatin E	Growth inhibition of human NCI-H460 cells after 48 hrs by SRB assay Growth inhibition of human NCI-H460 cells after 48 hrs by SRB assay	[PMID: 28926240]
NCI-H460	GI₅₀	0.533 nM Compound: Auristatin E	Growth inhibition of human NCI-H460 cells after 48 hrs by SRB assay Growth inhibition of human NCI-H460 cells after 48 hrs by SRB assay	[PMID: 28895394]
SF-268	GI₅₀	0.492 nM Compound: 2a	Growth inhibition of human SF268 cells incubated for 48 hrs by MTT assay Growth inhibition of human SF268 cells incubated for 48 hrs by MTT assay	[PMID: 28211277]
SF-268	GI₅₀	0.492 nM Compound: Auristatin E	Growth inhibition of human SF268 cells after 48 hrs by SRB assay Growth inhibition of human SF268 cells after 48 hrs by SRB assay	[PMID: 28926240]
SF-268	GI₅₀	0.492 nM Compound: Auristatin E	Growth inhibition of human SF268 cells after 48 hrs by SRB assay Growth inhibition of human SF268 cells after 48 hrs by SRB assay	[PMID: 28895394]

In Vitro

Auristatin E (1-10000 nM, 2 h) shows the cytotoxic effects on L2987 human lung adenocarcinoma cells and various hematologic cell lines^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Auristatin E (0.25 mg/kg, s.c., a single dose for 80 days) inhibits tumor growth in nude mice with subcutaneous L2987 human lung adenocarcinoma or 3396 human breast carcinoma xenografts^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Nude mice with subcutaneous L2987 human lung adenocarcinoma or 3396 human breast carcinoma xenografts^[2]
Dosage:	0.25 mg/kg
Administration:	s.c., a single dose for 80 days
Result:	Showed median tumor volume when nude mice with subcutaneous L2987 human lung adenocarcinoma or 3396 human breast carcinoma xenografts were injected with conjugates or drug.

Molecular Weight

732.01

Formula

C₄₀H₆₉N₅O₇

CAS No.

160800-57-7

Appearance

Solid

Color

White to light yellow

SMILES

CC(C)[C@H](N(C)C)C(N[C@H](C(N([C@@H]([C@@H](C)CC)[C@@H](CC(N1CCC[C@@]1([H])[C@H](OC)[C@@H](C)C(N[C@H](C)[C@H](C2=CC=CC=C2)O)=O)=O)OC)C)=O)C(C)C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (136.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3661 mL	6.8305 mL	13.6610 mL
5 mM	0.2732 mL	1.3661 mL	2.7322 mL
10 mM	0.1366 mL	0.6831 mL	1.3661 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

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Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.82%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (252 KB) HNMR (271 KB) RP-HPLC (267 KB) LCMS (92 KB)

Handling Instructions (2659 KB)

References

[1]. MADDON Paul J, et al. PSMA ANTIBODIES AND PROTEIN MULTIMER. 2002 October 23. WO 03/034903 A2

[2]. Peter D. Senter, et al. PENTAPEPTIDE COMPOUNDS AND USES RELATED THERETO. 2005 Jan. 13. US 2005/0009751 A l.

[3]. LAW, Che-Leung, et al. TREATMENT OF IMMUNOLOGICAL DISORDERS 5 USING ANTI-CD30 ANTIBODIES. 2002 November 20. WO 03/043583 A2.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.3661 mL	6.8305 mL	13.6610 mL	34.1525 mL
	5 mM	0.2732 mL	1.3661 mL	2.7322 mL	6.8305 mL
	10 mM	0.1366 mL	0.6831 mL	1.3661 mL	3.4153 mL
	15 mM	0.0911 mL	0.4554 mL	0.9107 mL	2.2768 mL
	20 mM	0.0683 mL	0.3415 mL	0.6831 mL	1.7076 mL
	25 mM	0.0546 mL	0.2732 mL	0.5464 mL	1.3661 mL
	30 mM	0.0455 mL	0.2277 mL	0.4554 mL	1.1384 mL
	40 mM	0.0342 mL	0.1708 mL	0.3415 mL	0.8538 mL
	50 mM	0.0273 mL	0.1366 mL	0.2732 mL	0.6831 mL
	60 mM	0.0228 mL	0.1138 mL	0.2277 mL	0.5692 mL
	80 mM	0.0171 mL	0.0854 mL	0.1708 mL	0.4269 mL
	100 mM	0.0137 mL	0.0683 mL	0.1366 mL	0.3415 mL

Auristatin E Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Auristatin E160800-57-7Microtubule/TubulinADC CytotoxinADC Payloadcytotoxicantitumor activityADCapoptosisxenograft mouse modelsB-cell malignanciesInhibitorinhibitorinhibit

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Auristatin E

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