B I09

Name: B I09
Brand: MedChemExpress
SKU: HY-107400
Rating: 4.5 (1 reviews)

Cat. No.: HY-107400 Purity: 98.69%: FAQs
Data Sheet SDS COA Handling Instructions

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B I09 is an IRE-1 RNase inhibitor, with an IC₅₀ of 1230 nM.

For research use only. We do not sell to patients.

B I09 Chemical Structure

CAS No. : 1607803-67-7

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Free Sample (0.1 - 0.5 mg)		Apply Now
5 mg	USD 130	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 480	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 680	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE B I09

•Patent. US20220381787A1.

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Description

B I09 is an IRE-1 RNase inhibitor, with an IC₅₀ of 1230 nM.

IC₅₀ & Target

IC50: 1230 nM (IRE-1 RNase)^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
Sf21	IC₅₀	1230 nM Compound: 28	Inhibition of human recombinant puritin-His-tagged IRE-1 RNase expressed in SF21 cells using XBP-1 RNA stem loop as substrate incubated for 30 mins prior to substrate addition measured after 2 hrs by FRET-suppression assay Inhibition of human recombinant puritin-His-tagged IRE-1 RNase expressed in SF21 cells using XBP-1 RNA stem loop as substrate incubated for 30 mins prior to substrate addition measured after 2 hrs by FRET-suppression assay	[PMID: 24749861]

In Vitro

B I09 is an IRE-1 RNase inhibitor, with an IC₅₀ of 1230 nM^[1]. Treatment of CLL cells with this inhibitor (B I09) mimick XBP-1 deficiency, including upregulation of IRE-1 expression and compromised BCR signaling. B I09 is highly effective in inhibiting splicing of XBP1 mRNA in human WaC3 cells and the expression of XBP-1s in LPS stimulated B cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

B I09 has a halflife of approximately 1.5 hours and reaches its peak concentration of approximately 39 μM in mouse plasma serum 15 minutes after administration. Administration of B I09 to CLL tumor-bearing mice suppress leukemic progression by inducing apoptosis and do not cause systemic toxicity^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

303.31

Formula

C₁₆H₁₇NO₅

CAS No.

1607803-67-7

Appearance

Solid

Color

Light yellow to brown

SMILES

OC1=CC=C(C2=C(CNCC2)C3=O)C(O3)=C1C4OCCCO4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

DMSO : 11.11 mg/mL (36.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2970 mL	16.4848 mL	32.9696 mL
5 mM	0.6594 mL	3.2970 mL	6.5939 mL
10 mM	0.3297 mL	1.6485 mL	3.2970 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.11 mg/mL (3.66 mM); Clear solution

This protocol yields a clear solution of ≥ 1.11 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (11.1 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*The compound is unstable in solutions, freshly prepared is recommended.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.69%

Select Batch:

Data Sheet (279 KB) SDS (252 KB)

COA (254 KB) RP-HPLC (203 KB)

Handling Instructions (2659 KB)

References

[1]. Ranatunga S, et al. Synthesis of novel tricyclic chromenone-based inhibitors of IRE-1 RNase activity. J Med Chem. 2014 May 22;57(10):4289-301. [Content Brief]

[2]. Tang CH, et al. Inhibition of ER stress-associated IRE-1/XBP-1 pathway reduces leukemic cell survival. J Clin Invest. 2014 Jun;124(6):2585-98. [Content Brief]

Animal Administration ^[2]	Mice^[2] Mice are intraperitoneally injected with B I09 (50 mg/kg) on the first 5 days of each week for 3 weeks^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Ranatunga S, et al. Synthesis of novel tricyclic chromenone-based inhibitors of IRE-1 RNase activity. J Med Chem. 2014 May 22;57(10):4289-301. [Content Brief] [2]. Tang CH, et al. Inhibition of ER stress-associated IRE-1/XBP-1 pathway reduces leukemic cell survival. J Clin Invest. 2014 Jun;124(6):2585-98. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2970 mL	16.4848 mL	32.9696 mL	82.4239 mL
	5 mM	0.6594 mL	3.2970 mL	6.5939 mL	16.4848 mL
	10 mM	0.3297 mL	1.6485 mL	3.2970 mL	8.2424 mL
	15 mM	0.2198 mL	1.0990 mL	2.1980 mL	5.4949 mL
	20 mM	0.1648 mL	0.8242 mL	1.6485 mL	4.1212 mL
	25 mM	0.1319 mL	0.6594 mL	1.3188 mL	3.2970 mL
	30 mM	0.1099 mL	0.5495 mL	1.0990 mL	2.7475 mL

B I09 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

B I091607803-67-7B I 09B I-09IRE1Inositol requiring enzyme 1Inhibitorinhibitorinhibit

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B I09

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B I09 Related Antibodies

1 Publications Citing Use of MCE B I09

Featured Recommendations

•Related Screening Libraries:

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Protocol

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References

Customer Review

B I09 Related Antibodies

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Complete Stock Solution Preparation Table

B I09 Related Classifications

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