Biotin-DADOO (Synonyms: Biotinyl-3,6-dioxaoctanediamine; EZ-Link Amine-PEO2-Biotin)

Name: Biotin-DADOO
Brand: MedChemExpress
SKU: HY-D0980
Rating: 4.5 (1 reviews)

Cat. No.: HY-D0980 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Biotin-DADOO is a biotinylation reagent, which can be used to synthesize a biotin-estradiol conjugate (i.e., biotin-DADOO-estradiol) to develop a direct, broad range enzyme immunoassay to measure plasma estradiol concentrations.

For research use only. We do not sell to patients.

Biotin-DADOO Chemical Structure

CAS No. : 138529-46-1

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100 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
250 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 460	In-stock	Estimated Time of Arrival: December 31
1 g	USD 600	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

Other Forms of Biotin-DADOO:

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Biological Activity
Purity & Documentation
References
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Description

Molecular Weight

374.50

Formula

C₁₆H₃₀N₄O₄S

CAS No.

138529-46-1

Appearance

Solid

Color

White to yellow

SMILES

O=C(NCCOCCOCCN)CCCC[C@@H]1SC[C@]([C@]1([H])N2)([H])NC2=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (267.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 100 mg/mL (267.02 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6702 mL	13.3511 mL	26.7023 mL
5 mM	0.5340 mL	2.6702 mL	5.3405 mL
10 mM	0.2670 mL	1.3351 mL	2.6702 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (267.02 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (267 KB) SDS (393 KB)

COA (243 KB) HNMR (308 KB)

Handling Instructions (2659 KB)

References

[1]. You L, et al. Scutellarin inhibits Hela cell growth and glycolysis by inhibiting the activity of pyruvate kinase M2. Bioorg Med Chem Lett. 2017 Dec 15;27(24):5404-5408. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	2.6702 mL	13.3511 mL	26.7023 mL	66.7557 mL
	5 mM	0.5340 mL	2.6702 mL	5.3405 mL	13.3511 mL
	10 mM	0.2670 mL	1.3351 mL	2.6702 mL	6.6756 mL
	15 mM	0.1780 mL	0.8901 mL	1.7802 mL	4.4504 mL
	20 mM	0.1335 mL	0.6676 mL	1.3351 mL	3.3378 mL
	25 mM	0.1068 mL	0.5340 mL	1.0681 mL	2.6702 mL
	30 mM	0.0890 mL	0.4450 mL	0.8901 mL	2.2252 mL
	40 mM	0.0668 mL	0.3338 mL	0.6676 mL	1.6689 mL
	50 mM	0.0534 mL	0.2670 mL	0.5340 mL	1.3351 mL
	60 mM	0.0445 mL	0.2225 mL	0.4450 mL	1.1126 mL
	80 mM	0.0334 mL	0.1669 mL	0.3338 mL	0.8344 mL
	100 mM	0.0267 mL	0.1335 mL	0.2670 mL	0.6676 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Biotin-DADOO Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Biotin-DADOO138529-46-1Biotinyl-3,6-dioxaoctanediamine EZ-Link Amine-PEO2-BiotinBiochemical Assay ReagentsInhibitorinhibitorinhibit

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Biotin-DADOO (Synonyms: Biotinyl-3,6-dioxaoctanediamine; EZ-Link Amine-PEO2-Biotin)

Customer Review

Other Forms of Biotin-DADOO:

Biotin-DADOO Related Antibodies

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Biotin-DADOO Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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