2. Cell Cycle/DNA Damage
  3. Deubiquitinase
  4. DUB-IN-3

DUB-IN-3 (compound 22c) is a potent and selective inhibitor of the deubiquitinating enzyme USP8 with an IC50 value of 0.56 μM. DUB-IN-3 is promising for research of cancer, neurodegenerative diseases, inflammation, viral infection and cardiovascular disease.

For research use only. We do not sell to patients.

DUB-IN-3 Chemical Structure

DUB-IN-3 Chemical Structure

CAS No. : 924296-17-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 462 In-stock
Solution
10 mM * 1 mL in DMSO USD 462 In-stock
Solid
5 mg USD 420 In-stock
10 mg USD 600 In-stock
50 mg USD 1400 In-stock
100 mg USD 1960 In-stock
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500 mg   Get quote  

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Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of DUB-IN-3:

DUB-IN-3 Related Antibodies

Top Publications Citing Use of Products

    DUB-IN-3 purchased from MedChemExpress. Usage Cited in: J Invest Dermatol. 2020 Jun;140(6):1154-1165.e5.  [Abstract]

    Western blot analysis of SCCRDEB4 cells treated with the USP8 inhibitor DUBs-IN-3 for 24 hours.

    View All Deubiquitinase Isoform Specific Products:

    View All Isoforms
    USP1 USP2 USP7 USP8 USP30 UCHL1

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    DUB-IN-3 (compound 22c) is a potent and selective inhibitor of the deubiquitinating enzyme USP8 with an IC50 value of 0.56 μM. DUB-IN-3 is promising for research of cancer, neurodegenerative diseases, inflammation, viral infection and cardiovascular disease[1].

    IC50 & Target

    USP7

    >100 μM (IC50)

    In Vitro

    DUB-IN-3 (10 nM-100 μM) affects the viability of HCT116 colon and PC-3 prostate cancer cell lines with IC50 values of 0.5-1.5 μM range[1].

    Inhibitory effect of DUB-IN-3 on USP7 and USP8 deubiquitinating activity (IC50: μM)[1]

    Compd USP7 USP8
    DUB-IN-3 >100 0.56

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DUB-IN-3 Related Antibodies
    Molecular Weight

    287.28

    Formula

    C16H9N5O
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    C=CCO/N=C1C2=C(N=C(C#N)C(C#N)=N2)C3=CC=CC=C/13
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 25 mg/mL (87.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.4810 mL 17.4049 mL 34.8098 mL
    5 mM 0.6962 mL 3.4810 mL 6.9620 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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    Working solution concentration: mg/mL
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.4810 mL 17.4049 mL 34.8098 mL 87.0245 mL
    5 mM 0.6962 mL 3.4810 mL 6.9620 mL 17.4049 mL
    10 mM 0.3481 mL 1.7405 mL 3.4810 mL 8.7024 mL
    15 mM 0.2321 mL 1.1603 mL 2.3207 mL 5.8016 mL
    20 mM 0.1740 mL 0.8702 mL 1.7405 mL 4.3512 mL
    25 mM 0.1392 mL 0.6962 mL 1.3924 mL 3.4810 mL
    30 mM 0.1160 mL 0.5802 mL 1.1603 mL 2.9008 mL
    40 mM 0.0870 mL 0.4351 mL 0.8702 mL 2.1756 mL
    50 mM 0.0696 mL 0.3481 mL 0.6962 mL 1.7405 mL
    60 mM 0.0580 mL 0.2901 mL 0.5802 mL 1.4504 mL
    80 mM 0.0435 mL 0.2176 mL 0.4351 mL 1.0878 mL
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    DUB-IN-3 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DUB-IN-3924296-17-3DeubiquitinaseDUBsUSP8cancerneurodegenerative diseasesinflammationviral infectioncardiovascular diseaseInhibitorinhibitorinhibit

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