Diloxanide furoate

Name: Diloxanide furoate
Brand: MedChemExpress
SKU: HY-B1147
Rating: 4.5 (1 reviews)

Cat. No.: HY-B1147 Purity: 99.62%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Diloxanide furoate is the proagent of Diloxanide. Diloxanide furoate is a potent and orally active anti-protozoal agent and can be used for the research of amebiasis, mild intestinal amebiasis or asymptomatic cyst carriers.

For research use only. We do not sell to patients.

Diloxanide furoate Chemical Structure

CAS No. : 3736-81-0

Size	Price	Stock	Quantity
10 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Diloxanide furoate:

Diloxanide furoate (Standard) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Amebae

In Vitro

Diloxanide furoate (1.95-2.5 μg/ml) has potent activity against different strains of E. histolytica, it is against BYso (grown with bacteria), SFL3 (grown with Chrithidia sp.), and SFL3 (grown axenically) with MIC values of 2.5 μg/ml, 2.5 μg/ml and 1.95 μg/ml, respectively^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Diloxanide furoate (oral administraion; 75-200 mg/kg; 3 days; once daily) is effective at different dose of dayin weanling rats. At 200 mg/kg, 100% of the treated rats are cured and no amoebic lesions are observed in the caecum. Besides, 85%,77%, and 44.4% of the treated rats are cured at the dose 150 mg/kg, 100 mg/kg, and 75 mg/kg, respectively. The ED₅₀ value is 77.9 mg/kg for this agent in rats^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Weanling Wistar rats^[2]
Dosage:	200 mg/kg; 150 mg/kg; 100 mg/kg; 75 mg/kg
Administration:	Oral administraion; 75-200 mg/kg; 3 days; once daily
Result:	Was against intestinal (caecal) amoebiasis in rats.

Molecular Weight

328.15

Formula

C₁₄H₁₁Cl₂NO₄

CAS No.

3736-81-0

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=CC=CO1)OC2=CC=C(N(C(C(Cl)Cl)=O)C)C=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 50 mg/mL (152.37 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 20 mg/mL (60.95 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0474 mL	15.2369 mL	30.4739 mL
5 mM	0.6095 mL	3.0474 mL	6.0948 mL
10 mM	0.3047 mL	1.5237 mL	3.0474 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.80%

Select Batch:

Data Sheet (273 KB) SDS (392 KB)

COA (247 KB) LCMS (121 KB)

Handling Instructions (2659 KB)

References

[1]. VA CLASSIFICATION. Diloxanide (Systemic)

[2]. D K Chatterjee, et al. Antiamoebic activity of chonemorphine, a steroidal alkaloid, in experimental models. Parasitol Res. 1987;74(1):30-3. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	3.0474 mL	15.2369 mL	30.4739 mL	76.1847 mL
	5 mM	0.6095 mL	3.0474 mL	6.0948 mL	15.2369 mL
	10 mM	0.3047 mL	1.5237 mL	3.0474 mL	7.6185 mL
	15 mM	0.2032 mL	1.0158 mL	2.0316 mL	5.0790 mL
	20 mM	0.1524 mL	0.7618 mL	1.5237 mL	3.8092 mL
	25 mM	0.1219 mL	0.6095 mL	1.2190 mL	3.0474 mL
	30 mM	0.1016 mL	0.5079 mL	1.0158 mL	2.5395 mL
	40 mM	0.0762 mL	0.3809 mL	0.7618 mL	1.9046 mL
	50 mM	0.0609 mL	0.3047 mL	0.6095 mL	1.5237 mL
	60 mM	0.0508 mL	0.2539 mL	0.5079 mL	1.2697 mL
DMSO	80 mM	0.0381 mL	0.1905 mL	0.3809 mL	0.9523 mL
DMSO	100 mM	0.0305 mL	0.1524 mL	0.3047 mL	0.7618 mL