EC-17 disodium salt

Name: EC-17 disodium salt
Brand: MedChemExpress
SKU: HY-13615A
Rating: 4.5 (1 reviews)

Cat. No.: HY-13615A Purity: 96.21%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

EC-17 (disodium salt) is a folate receptor alpha (FRα) targeting contrast agent with fluorescent properties in the visible light spectrum. The peak excitation and emission wavelengths of EC-17 are 470/520 nm.

For research use only. We do not sell to patients.

EC-17 disodium salt Chemical Structure

CAS No. : 910661-33-5

Size	Price	Stock	Quantity
5 mg	USD 850	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1450	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 2850	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 4550	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of EC-17 disodium salt:

Folate-FTIC Get quote

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Folate receptor alpha^[1]

In Vitro

EC-17 contains the fluorescein fluorochrome and has a spectral wavelength of 490-530 nm. EC-17 is synthesized by a folate (vitamin B9) and fluorescein isothiocyanate (FITC) conjugated through an ethylenediamine spacer to produce folate-FITC, with a molecular weight of 917 kDa. FITC is a derivative of fluorescein functionalized with an isothiocyanate reactive group. The folate-FITC conjugate forms a negatively charged fluorescent molecule that specifically targets cell-surface FRα and is subsequently internalized into the cytoplasm. The signal-to-background ratio (SBR) of EC-17 for HeLa cells range from 0.97 to 7.32 depending on the molarity and concentration of cancer cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The mean fluorescence signal from the animals injected with EC-17 to be 42,234 ± 12,234 au^[1]. Fluorescence microscopy for folate-FITC shows a strong signal in all malignant tumors with FR-α expression and no signal in FR-α–negative malignant or benign lesions^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

916.82

Formula

C₄₂H₃₄N₁₀Na₂O₁₀S

CAS No.

910661-33-5

Appearance

Solid

Color

Yellow to orange

SMILES

S=C(NCCNC(CC[C@@H](C(O[Na])=O)NC(C1=CC=C(NCC2=CN=C3N=C(N)NC(C3=N2)=O)C=C1)=O)=O)NC4=CC5=C(C6(C7=C(OC8=C6C=CC(O)=C8)C=C(O[Na])C=C7)OC5=O)C=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 40 mg/mL (43.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.0907 mL	5.4536 mL	10.9073 mL
5 mM	0.2181 mL	1.0907 mL	2.1815 mL
10 mM	0.1091 mL	0.5454 mL	1.0907 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 1.6 mg/mL (1.75 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1.6 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 1.6 mg/mL (1.75 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1.6 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 96.30%

Select Batch:

Data Sheet (276 KB) SDS (252 KB)

COA (253 KB) HNMR (279 KB) LCMS (141 KB)

Handling Instructions (2659 KB)

References

[1]. Tummers QR, et al. Intraoperative imaging of folate receptor alpha positive ovarian and breast cancer using the tumor specific agent EC17. Oncotarget. 2016 May 31;7(22):32144-55. [Content Brief]

Cell Assay ^[1]	KB, HeLa, and TC1 cells are plated on a cell culture treated 6-well plate and incubated for 16 hours. Once confluent, EC-17 is added cells. The cells are incubated and sealed in a light-protected environment for 45 minutes. Cells are then washed 3 times with PBS and plated and underwent fluorescence microscopy^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Mice^[1] Mice are injected subcutaneously in the flank with 1.2×10⁶ TC1 cells (C57BL/6 mice), 1.0×10⁶ HeLa cells (NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ mice), or 1.0×106 KB cells (NOD.Cg-Prkdc^scid, Il2rg^tm1Wjl/SzJ mice). Once tumor volume reached approximately 300 mm³ half of the mice are injected with 0.1 mg/kg of EC-17 and the other half with 0.1 mg/kg of OTL38 via tail vein. Three hours later, the fluorescence of tumors is measured using Flocam^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Tummers QR, et al. Intraoperative imaging of folate receptor alpha positive ovarian and breast cancer using the tumor specific agent EC17. Oncotarget. 2016 May 31;7(22):32144-55. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.0907 mL	5.4536 mL	10.9073 mL	27.2682 mL
	5 mM	0.2181 mL	1.0907 mL	2.1815 mL	5.4536 mL
	10 mM	0.1091 mL	0.5454 mL	1.0907 mL	2.7268 mL
	15 mM	0.0727 mL	0.3636 mL	0.7272 mL	1.8179 mL
	20 mM	0.0545 mL	0.2727 mL	0.5454 mL	1.3634 mL
	25 mM	0.0436 mL	0.2181 mL	0.4363 mL	1.0907 mL
	30 mM	0.0364 mL	0.1818 mL	0.3636 mL	0.9089 mL
	40 mM	0.0273 mL	0.1363 mL	0.2727 mL	0.6817 mL