2. Anti-infection Autophagy Apoptosis Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Parasite Autophagy Apoptosis Reactive Oxygen Species Antibiotic
  4. Avermectin B1

Avermectin B1  (Synonyms: Abamectin; Avermectin B1a-Avermectin B1b mixt.)

Cat. No.: HY-15311 Purity: 98.36%
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Avermectin B1 (Abamectin) is a mixture of two similar segments of avermectin. Avermectin B1 is an orally anti-infection agent, which can be used in the research of parasitic worms, insect pests, agriculture and animal husbandry. Avermectin B1 can also induce the production of ROS and induces cytotoxicity, apoptosis and autophagy.

For research use only. We do not sell to patients.

Avermectin B1 Chemical Structure

Avermectin B1 Chemical Structure

CAS No. : 71751-41-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 68 In-stock
Solution
10 mM * 1 mL in DMSO USD 68 In-stock
Solid
100 mg USD 50 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Avermectin B1:

Avermectin B1 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

Avermectin B1 (Abamectin) is a mixture of two similar segments of avermectin. Avermectin B1 is an orally anti-infection agent, which can be used in the research of parasitic worms, insect pests, agriculture and animal husbandry. Avermectin B1 can also induce the production of ROS and induces cytotoxicity, apoptosis and autophagy[1][2][4].

In Vitro

Avermectin B1 (0-80 μM, 12 h) induces cytotoxicity through MAPK and ATM/ATR pathway in mouse embryonic fibroblast (MEF) cells, and induces ROS-mediated DNA damage[1].
Avermectin B1 (36 μg/mL, 72 h) has strong nematicidal efect of on G. pallida in aqueous solution, and negatively influences viability and infectivity of G. pallida J2 detected in potato roots(cv. Spunta)[2].
Abamectin (10 μM, 24 h) induces significant cytotoxicity by overproduction of ROS in haemocytes of Erocheir sinensis[3].
Abamectin (4 μM, 24 h) induces apoptosis and autophagy by inhibiting ROS-mediated PI3K/AKT signaling in MGC803 cells[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Avermectin B1 Related Antibodies

Cell Viability Assay[1]

Cell Line: Mouse embryonic fibroblast (MEF) cells
Concentration: 0, 0.5, 5, 10, 20, 40, 80 μM
Incubation Time: 12 h
Result: Reduced cell viability with an IC50 value of 45.6 μM.

Western Blot Analysis[3]

Cell Line: MGC803 cells
Concentration: 0-4 μM
Incubation Time: 24 h
Result: Increased active caspase-3 and expression of Bax/Bcl-2, decreased MMP in a dose-dependent manner.
In Vivo

Avermectin B1 (oral administration, 0.2 mg/kg for a single dose) is highly efficacious against intestinal strongyles and Onchocera microfilaria in horses[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Horses (had a pruritic dermatosis)[5]
Dosage: 0.2 mg/kg, a single dose.
Administration: Oral administration
Result: Decreased in mean strongyle egg counts 14, 28 and 42 d after treatment, and resulted in zero microfilaria counts in all horses 14 d after treatment.
Animal Model: Healthy adult female sheep (Pharmacokinetic assay)[6]
Dosage: 0.2 mg/kg
Administration: Subcutaneous administration (in the left neck area of each sheep)
Result: Pharmacokinetic profiles of Avermectin B1
Parameters Mean
Kcl (/day) 0.17
t1/2cl (day) 4.36
Kab (/day) 0.24
t1/2ab (day) 3.15
Cmax (ng/mL) 6.24
tmax (day) 4.20
AUC (0-27) (ng/day/mL) 80.2
AUC(0-∞) (ng/day/mL) 84.7
MRT (day) 8.80
Clinical Trial
Molecular Weight

873.09

Formula

C48H72O14 (for Avermectin B1a)
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@@H](CC)[C@]1([H])[C@@H](C)C=C[C@@]2(O[C@@]3([H])C[C@]([H])(OC([C@@]4([H])[C@@](/C(CO5)=C/C=C/[C@H](C)[C@H](O[C@@]6([H])C[C@H](OC)[C@@H](O[C@]7([H])O[C@@H](C)[C@H](O)[C@@H](OC)C7)[C@H](C)O6)/C(C)=C/C3)(O)[C@@]5([H])[C@H](O)C(C)=C4)=O)C2)O1.C[C@H]8C=C[C@@]9(O[C@@]%10([H])C[C@]([H])(OC([C@@]%11([H])[C@@](/C(CO%12)=C/C=C/[C@H](C)[C@H](O[C@@]%13([H])C[C@H](OC)[C@@H](O[C@]%14([H])O[C@@H](C)[C@H](O)[C@@H](OC)C%14)[C@H](C)O%13)/C(C)=C/C%10)(O)[C@@]%12([H])[C@H](O)C(C)=C%11)=O)C9)O[C@@H]8C(C)C
Structure Classification
Initial Source

Streptomyces avermitilis
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (286.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1454 mL 5.7268 mL 11.4536 mL
5 mM 0.2291 mL 1.1454 mL 2.2907 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (2.38 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.08 mg/mL (2.38 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1454 mL 5.7268 mL 11.4536 mL 28.6339 mL
5 mM 0.2291 mL 1.1454 mL 2.2907 mL 5.7268 mL
10 mM 0.1145 mL 0.5727 mL 1.1454 mL 2.8634 mL
15 mM 0.0764 mL 0.3818 mL 0.7636 mL 1.9089 mL
20 mM 0.0573 mL 0.2863 mL 0.5727 mL 1.4317 mL
25 mM 0.0458 mL 0.2291 mL 0.4581 mL 1.1454 mL
30 mM 0.0382 mL 0.1909 mL 0.3818 mL 0.9545 mL
40 mM 0.0286 mL 0.1432 mL 0.2863 mL 0.7158 mL
50 mM 0.0229 mL 0.1145 mL 0.2291 mL 0.5727 mL
60 mM 0.0191 mL 0.0954 mL 0.1909 mL 0.4772 mL
80 mM 0.0143 mL 0.0716 mL 0.1432 mL 0.3579 mL
100 mM 0.0115 mL 0.0573 mL 0.1145 mL 0.2863 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Avermectin B171751-41-2Abamectin Avermectin B1a-Avermectin B1b mixt.Avermectin B 1Avermectin B-1ParasiteAutophagyApoptosisReactive Oxygen SpeciesAntibioticROSDNA damageautophagyapoptosisstrongylesparasiteinsecticideanti-infectionInhibitorinhibitorinhibit

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