FIPI (Synonyms: 5-Fluoro-2-indolyl deschlorohalopemide)

Name: FIPI
Brand: MedChemExpress
SKU: HY-12807
Rating: 5.0 (6 reviews)

Cat. No.: HY-12807 Purity: 98.00%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

FIPI is a phospholipase D (PLD) inhibitor with an IC₅₀ for PLD1 and PLD2 of about 25 nM. FIPI regulates cytoskeletal recombination, cell diffusion and chemotaxis. FIPI can be used in cancer research. In addition, FIPI can enhance the secretion and aggregation of platelet dense particles, inhibit thrombosis, reduce ischemic stroke infarct volume and improve nerve function.

For research use only. We do not sell to patients.

FIPI Chemical Structure

CAS No. : 939055-18-2

Size	Stock	Quantity

Free Sample (0.1 - 0.5 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of FIPI:

FIPI hydrochloride Get quote

4 Publications Citing Use of MCE FIPI

: FIPI purchased from MedChemExpress. Usage Cited in: Anal Chem. 2018 Jun 5;90(11):6742-6748. [Abstract]; Typical CSLM images of MDA-MB-231 cells incubated with 50 μg/mL RhB-PL-Ti₃C₂ for 4 h at 37°C in the absence (I) and presence of 1.0 mg/mL FIPI (II), and CSLM images of MCF-7 cells (III) incubated with 50 μg/mL RhB-PL-Ti₃C₂ for 4 h at 37°C.

: FIPI purchased from MedChemExpress. Usage Cited in: J Cell Sci. 2018 Mar 16;131(6). pii: jcs207217. [Abstract]; ER:Ras IMR90 cells are treated with solvent or FIPI (250 nM, 2h).Western blotting are performed.

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Phospholipase A PLD Phospholipase C

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

PLD1

PLD2

Cellular Effect

Cell Line	Type	Value	Description	References
Calu-1	IC₅₀	1 nM Compound: 14	Inhibition of PLD1 in human Calu-1 cells assessed as decrease in phosphatidylbutanol-[d9] production after 30 mins by mass spectrometric analysis Inhibition of PLD1 in human Calu-1 cells assessed as decrease in phosphatidylbutanol-[d9] production after 30 mins by mass spectrometric analysis	[PMID: 19136975]
HEK293	IC₅₀	300 nM Compound: 14	Inhibition of GFP-tagged human PLD2 expressed in human HEK293 cells assessed as decrease in phosphatidylbutanol-[d9] production after 30 mins by mass spectrometric analysis Inhibition of GFP-tagged human PLD2 expressed in human HEK293 cells assessed as decrease in phosphatidylbutanol-[d9] production after 30 mins by mass spectrometric analysis	[PMID: 19136975]

In Vitro

FIPI (750 nM; 30 min) effectively blocks the production of phosphatidic acid by PLD stimulated by PMA (HY-18739) in HEK293 cells^[1].
FIPI (750 nM; 1-4 h) promotes the diffusion of CHO cells and inhibits the chemotaxis of neutrophils^[1].
FIPI (1-100 nM; 1-45 min) inhibits cell migration and calcium release in EGF-induced MDA-MB-468-NEO (EGFR positive) and MDA-MB-468-HER2 (EGFR and HER2 positive)^[2].
FIPI (10 μM; 2.5 min) enhances the secretion and aggregation of dense particles in platelets, and increases the phosphorylation of protein kinase C substrate Pleckstrin^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

FIPI (3 mg/kg; intraperitoneal injection; 2 times) shows protective effect in carotid thrombosis model and ischemic stroke model^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Ferric chloride (HY-Y0266) or transient middle cerebral artery occlusion treated mice^[4]
Dosage:	3 mg/kg
Administration:	Intraperitoneal injection (i.p.); 2 times, 12 h apart
Result:	Delayed the thrombus formation and only 3 of 9 vessels occluded during the 30-minute observation period in arterial thrombosis models. Reduced the infarct volumes, improved motor and overall nerve function and did not impair normal hemostasis in ischemic stroke models.

Molecular Weight

421.47

Formula

C₂₃H₂₄FN₅O₂

CAS No.

939055-18-2

Appearance

Solid

Color

Light yellow to khaki

SMILES

O=C(NCCN1CCC(CC1)N2C3=CC=CC=C3NC2=O)C4=CC5=C(C=CC(F)=C5)N4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 14.67 mg/mL (34.81 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3726 mL	11.8632 mL	23.7265 mL
5 mM	0.4745 mL	2.3726 mL	4.7453 mL
10 mM	0.2373 mL	1.1863 mL	2.3726 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.49%

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Data Sheet (278 KB) SDS (252 KB)

COA (253 KB) HNMR (153 KB) LCMS (355 KB)

Handling Instructions (2659 KB)

References

[1]. Su W, et al. 5-Fluoro-2-indolyl des-chlorohalopemide (FIPI), a phospholipase D pharmacological inhibitor that alters cell spreading and inhibits chemotaxis. Mol Pharmacol. 2009 Mar;75(3):437-46. [Content Brief]

[2]. Stricker HM, et al. The phospholipase D inhibitor FIPI potently blocks EGF-induced calcium signaling in human breast cancer cells. Cell Commun Signal. 2021 Apr 8;19(1):43. [Content Brief]

[3]. Elvers M, et al. A novel role for phospholipase D as an endogenous negative regulator of platelet sensitivity. Cell Signal. 2012 Sep;24(9):1743-52. [Content Brief]

[4]. Stegner D, et al. Pharmacological inhibition of phospholipase D protects mice from occlusive thrombus formation and ischemic stroke--brief report. Arterioscler Thromb Vasc Biol. 2013 Sep;33(9):2212-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3726 mL	11.8632 mL	23.7265 mL	59.3162 mL
	5 mM	0.4745 mL	2.3726 mL	4.7453 mL	11.8632 mL
	10 mM	0.2373 mL	1.1863 mL	2.3726 mL	5.9316 mL
	15 mM	0.1582 mL	0.7909 mL	1.5818 mL	3.9544 mL
	20 mM	0.1186 mL	0.5932 mL	1.1863 mL	2.9658 mL
	25 mM	0.0949 mL	0.4745 mL	0.9491 mL	2.3726 mL
	30 mM	0.0791 mL	0.3954 mL	0.7909 mL	1.9772 mL

FIPI Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FIPI939055-18-25-Fluoro-2-indolyl deschlorohalopemidePhospholipaseCarotid thrombosis modelIschemic stroke modelHEK293 cellsCHO cellsMDA-MB-468 cellsInhibitorinhibitorinhibit

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FIPI (Synonyms: 5-Fluoro-2-indolyl deschlorohalopemide)

Customer Review

Other Forms of FIPI:

FIPI Related Antibodies

4 Publications Citing Use of MCE FIPI

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Phospholipase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

FIPI Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

FIPI Related Classifications

Keywords:

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Customer Review

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