1. Metabolic Enzyme/Protease
  2. Phospholipase
  3. FIPI

FIPI  (Synonyms: 5-Fluoro-2-indolyl deschlorohalopemide)

Cat. No.: HY-12807 Purity: 99.82%
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FIPI is a phospholipase D (PLD) inhibitor with an IC50 for PLD1 and PLD2 of about 25 nM. FIPI regulates cytoskeletal recombination, cell diffusion and chemotaxis. FIPI can be used in cancer research. In addition, FIPI can enhance the secretion and aggregation of platelet dense particles, inhibit thrombosis, reduce ischemic stroke infarct volume and improve nerve function.

For research use only. We do not sell to patients.

FIPI Chemical Structure

FIPI Chemical Structure

CAS No. : 939055-18-2

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Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of FIPI:

Top Publications Citing Use of Products

    FIPI purchased from MedChemExpress. Usage Cited in: Anal Chem. 2018 Jun 5;90(11):6742-6748.  [Abstract]

    Typical CSLM images of MDA-MB-231 cells incubated with 50 μg/mL RhB-PL-Ti3C2 for 4 h at 37°C in the absence (I) and presence of 1.0 mg/mL FIPI (II), and CSLM images of MCF-7 cells (III) incubated with 50 μg/mL RhB-PL-Ti3C2 for 4 h at 37°C.

    FIPI purchased from MedChemExpress. Usage Cited in: J Cell Sci. 2018 Mar 16;131(6). pii: jcs207217.  [Abstract]

    ER:Ras IMR90 cells are treated with solvent or FIPI (250 nM, 2h).Western blotting are performed.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    FIPI is a phospholipase D (PLD) inhibitor with an IC50 for PLD1 and PLD2 of about 25 nM. FIPI regulates cytoskeletal recombination, cell diffusion and chemotaxis. FIPI can be used in cancer research. In addition, FIPI can enhance the secretion and aggregation of platelet dense particles, inhibit thrombosis, reduce ischemic stroke infarct volume and improve nerve function[1][2][3][4].

    IC50 & Target[1]

    PLD1

     

    PLD2

     

    Cellular Effect
    Cell Line Type Value Description References
    Calu-1 IC50
    1 nM
    Compound: 14
    Inhibition of PLD1 in human Calu-1 cells assessed as decrease in phosphatidylbutanol-[d9] production after 30 mins by mass spectrometric analysis
    Inhibition of PLD1 in human Calu-1 cells assessed as decrease in phosphatidylbutanol-[d9] production after 30 mins by mass spectrometric analysis
    [PMID: 19136975]
    HEK293 IC50
    300 nM
    Compound: 14
    Inhibition of GFP-tagged human PLD2 expressed in human HEK293 cells assessed as decrease in phosphatidylbutanol-[d9] production after 30 mins by mass spectrometric analysis
    Inhibition of GFP-tagged human PLD2 expressed in human HEK293 cells assessed as decrease in phosphatidylbutanol-[d9] production after 30 mins by mass spectrometric analysis
    [PMID: 19136975]
    In Vitro

    FIPI (750 nM; 30 min) effectively blocks the production of phosphatidic acid by PLD stimulated by PMA (HY-18739) in HEK293 cells[1].
    FIPI (750 nM; 1-4 h) promotes the diffusion of CHO cells and inhibits the chemotaxis of neutrophils[1].
    FIPI (1-100 nM; 1-45 min) inhibits cell migration and calcium release in EGF-induced MDA-MB-468-NEO (EGFR positive) and MDA-MB-468-HER2 (EGFR and HER2 positive)[2].
    FIPI (10 μM; 2.5 min) enhances the secretion and aggregation of dense particles in platelets, and increases the phosphorylation of protein kinase C substrate Pleckstrin[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    FIPI (3 mg/kg; intraperitoneal injection; 2 times) shows protective effect in carotid thrombosis model and ischemic stroke model[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Ferric chloride (HY-Y0266) or transient middle cerebral artery occlusion treated mice[4]
    Dosage: 3 mg/kg
    Administration: Intraperitoneal injection (i.p.); 2 times, 12 h apart
    Result: Delayed the thrombus formation and only 3 of 9 vessels occluded during the 30-minute observation period in arterial thrombosis models.
    Reduced the infarct volumes, improved motor and overall nerve function and did not impair normal hemostasis in ischemic stroke models.
    Molecular Weight

    421.47

    Formula

    C23H24FN5O2

    CAS No.
    Appearance

    Solid

    Color

    Light yellow to khaki

    SMILES

    O=C(C(N1)=CC2=C1C=CC(F)=C2)NCCN3CCC(N4C5=CC=CC=C5NC4=O)CC3

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 14.67 mg/mL (34.81 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.3726 mL 11.8632 mL 23.7265 mL
    5 mM 0.4745 mL 2.3726 mL 4.7453 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.82%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.3726 mL 11.8632 mL 23.7265 mL 59.3162 mL
    5 mM 0.4745 mL 2.3726 mL 4.7453 mL 11.8632 mL
    10 mM 0.2373 mL 1.1863 mL 2.3726 mL 5.9316 mL
    15 mM 0.1582 mL 0.7909 mL 1.5818 mL 3.9544 mL
    20 mM 0.1186 mL 0.5932 mL 1.1863 mL 2.9658 mL
    25 mM 0.0949 mL 0.4745 mL 0.9491 mL 2.3726 mL
    30 mM 0.0791 mL 0.3954 mL 0.7909 mL 1.9772 mL
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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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