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  4. Formamide

Formamide  (Synonyms: Methanamide; Formimidic acid)

Cat. No.: HY-Y0842 Purity: 99.90%
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Formamide is an effective DNA denaturant that significantly reduces the stability of DNA in buffer solutions. It induces apoptosis by promoting the formation of cyclic nucleotides and the phosphorylation and dephosphorylation processes of nucleotides, making it useful in cancer research. Formamide can also be used as a decalcifying agent for rat cardiac cells. Additionally, formamide is widely used as a solvent or chemical raw material for ion compounds, resins, and plasticizers.

For research use only. We do not sell to patients.

Formamide Chemical Structure

Formamide Chemical Structure

CAS No. : 75-12-7

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Based on 1 publication(s) in Google Scholar

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Formamide Related Antibodies

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Description

Formamide is an effective DNA denaturant that significantly reduces the stability of DNA in buffer solutions. It induces apoptosis by promoting the formation of cyclic nucleotides and the phosphorylation and dephosphorylation processes of nucleotides, making it useful in cancer research. Formamide can also be used as a decalcifying agent for rat cardiac cells. Additionally, formamide is widely used as a solvent or chemical raw material for ion compounds, resins, and plasticizers.[1][2][3][4][5][6][7]

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Formamide (15 mol/L, 15 minutes) can induce the loss of T-tubules in rat cardiac myocytes[4].
Formamide (25 mL, 10 minutes) induces DNA denaturation in apoptotic cells (Staurosporine (HY-15141) treated breast cancer MDA-468 cells, leukemia MOLT-4 cells, and drug-resistant subline MOLT-4/R), but has no effect on the DNA of non-apoptotic cells (actively proliferating MDA-468 and MOLT-4 cells)[5].
Formamide (0.31-0.5 M, 3 hours) inhibits interkinetic nuclear migration and reduces microtubule quantity in chick embryo neuroepithelial cells[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Formamide Related Antibodies

Cell Migration Assay [6]

Cell Line: Neuroepithelial cells in the chick embryo
Concentration: 0.1, 0.25, 0.31, 0.37, 0.43, 0.5 M
Incubation Time: 3 h
Result: Only affected mitosis which was blocked in metaphase so that mitotic figures accumulated near the neuro-coele (0.25 M).
Totally inhibited interkinetic nuclear migration and affected only slightly the cell asymmetry (0.31 M).
Considerably reduced the amount of cytoplasmic microtubules and that the cells became round (0.37 M).
Caused all microtubules had disappeared and all cells were spherical (0.5 M).
In Vivo

Formamide (80-160 mg/kg, oral, once daily, 5 days a week, for 2 weeks, 3 months, or 2 years) exhibits carcinogenic activity in male B6C3F1 mice[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: B6C3F1 mice[7]
Dosage: 0, 10, 20, 40, 80, 160 mg/kg
Administration: Oral Gavage (p.o.), once daily, 5 days a week for 2 weeks, 3 months, or 2 years
Result: Resulted in a significant increase in the incidence of liver hemangiosarcoma, particularly in the 40 mg/kg and 80 mg/kg treatment groups.
Molecular Weight

45.04

Formula

CH3NO
CAS No.
Appearance

Liquid (Density: 1.13 g/cm3)

Color

Colorless to light yellow

SMILES

O=CN
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (2220.25 mM; Need ultrasonic)

DMSO : 100 mg/mL (2220.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.90%

SDS (418 KB)

COA (244 KB) HNMR (236 KB) GC (143 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 22.2025 mL 111.0124 mL 222.0249 mL 555.0622 mL
5 mM 4.4405 mL 22.2025 mL 44.4050 mL 111.0124 mL
10 mM 2.2202 mL 11.1012 mL 22.2025 mL 55.5062 mL
15 mM 1.4802 mL 7.4008 mL 14.8017 mL 37.0041 mL
20 mM 1.1101 mL 5.5506 mL 11.1012 mL 27.7531 mL
25 mM 0.8881 mL 4.4405 mL 8.8810 mL 22.2025 mL
30 mM 0.7401 mL 3.7004 mL 7.4008 mL 18.5021 mL
40 mM 0.5551 mL 2.7753 mL 5.5506 mL 13.8766 mL
50 mM 0.4440 mL 2.2202 mL 4.4405 mL 11.1012 mL
60 mM 0.3700 mL 1.8502 mL 3.7004 mL 9.2510 mL
80 mM 0.2775 mL 1.3877 mL 2.7753 mL 6.9383 mL
100 mM 0.2220 mL 1.1101 mL 2.2202 mL 5.5506 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Formamide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Formamide75-12-7Methanamide Formimidic acidBiochemical Assay ReagentsApoptosisDenaturantdnasolventchemical feedstockInhibitorinhibitorinhibit

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