Gramicidin

Name: Gramicidin
Brand: MedChemExpress
SKU: HY-P0163
Rating: 5.0 (4 reviews)

Cat. No.: HY-P0163 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Gramicidin is an antimicrobial peptide assembling as channels in membranes and increasing their permeability towards cations.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Gramicidin Chemical Structure

CAS No. : 1405-97-6

Size	Price	Stock	Quantity
25 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 4 publication(s) in Google Scholar

4 Publications Citing Use of MCE Gramicidin

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Gramicidin is an antimicrobial peptide assembling as channels in membranes and increasing their permeability towards cations.

IC₅₀ & Target

Bacterial^[1]

In Vitro

Gramicidin is an antimicrobial peptide assembling as channels in membranes and increasing their permeability towards cations. There is poor Gramicidin activity against E. coli and S. cerevisae as shown from the survival of microbes (%) over a range of Gramicidin concentrations, in contrast with the action of Gramicidin on S. aureus. The microbicidal activity occurs over a range of low Gramicidin and DODAB concentrations which are not toxic to S. cerevisae^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

1882.30

Formula

C₉₉H₁₄₀N₂₀O₁₇

CAS No.

1405-97-6

Appearance

Solid

Color

White to off-white

Structure Classification

Initial Source

Microorganisms

membrane pentadecapeptide

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (53.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.5313 mL	2.6563 mL	5.3126 mL
5 mM	0.1063 mL	0.5313 mL	1.0625 mL
10 mM	0.0531 mL	0.2656 mL	0.5313 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 5% DMSO 40% PEG300 5% Tween-80 50% Saline
Solubility: 10 mg/mL (5.31 mM); Suspended solution; Need ultrasonic
Protocol 2

Add each solvent one by one: 5% DMSO 95% Corn Oil
Solubility: 10 mg/mL (5.31 mM); Suspended solution; Need ultrasonic
Protocol 3

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (1.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 4

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (1.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (271 KB) SDS (393 KB)

COA (244 KB)

Handling Instructions (2659 KB)

References

[1]. Ragioto DA, et al. Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents. Int J Nanomedicine. 2014 Jun 30;9:3183-92. [Content Brief]

Cell Assay
^[1]

Microbes and dispersions are mixed and interacted for one hour before being diluted up to 20,000-fold for plating of 0.1 mL of each in triplicate and incubation (24 hours for 37°C) for CFU counting. Cell survival (%), taken as the mean±standard deviation, is plotted against DODAB and/or Gramicidin concentration. As a control for cell viability in the absence of DODAB or DODAB/Gramicidin dispersions, a standard bacterial suspension is added to 0.264 M D-glucose solution, diluted, and spreaded on the agar plate^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Ragioto DA, et al. Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents. Int J Nanomedicine. 2014 Jun 30;9:3183-92. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.5313 mL	2.6563 mL	5.3126 mL	13.2816 mL
	5 mM	0.1063 mL	0.5313 mL	1.0625 mL	2.6563 mL
	10 mM	0.0531 mL	0.2656 mL	0.5313 mL	1.3282 mL
	15 mM	0.0354 mL	0.1771 mL	0.3542 mL	0.8854 mL
	20 mM	0.0266 mL	0.1328 mL	0.2656 mL	0.6641 mL
	25 mM	0.0213 mL	0.1063 mL	0.2125 mL	0.5313 mL
	30 mM	0.0177 mL	0.0885 mL	0.1771 mL	0.4427 mL
	40 mM	0.0133 mL	0.0664 mL	0.1328 mL	0.3320 mL
	50 mM	0.0106 mL	0.0531 mL	0.1063 mL	0.2656 mL

Gramicidin Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Gramicidin1405-97-6BacterialAntibioticInhibitorinhibitorinhibit

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Gramicidin

Customer Review

Gramicidin Related Antibodies

4 Publications Citing Use of MCE Gramicidin

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Antibiotic Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Gramicidin Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Gramicidin Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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