Guanine

Name: Guanine
Brand: MedChemExpress
SKU: HY-Y1055
Rating: 5.0 (3 reviews)

Cat. No.: HY-Y1055 Purity: ≥98.0%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Guanine is one of the fundamental components of nucleic acids (DNA and RNA). Guanine is a purine derivative, consisting of a fused pyrimidine-imidazole ring system with conjugated double bonds. Guanine has the potential to serve as a large-capacity N pool. Guanine has cytotoxic, antinociceptive and neuroprotective effects.

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Guanine Chemical Structure

CAS No. : 73-40-5

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Guanine:

3 Publications Citing Use of MCE Guanine

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Human Endogenous Metabolite

Microbial Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
A-431	IC₅₀	> 100 μg/mL Compound: guanine	Inhibition of phosphatidylinositol 4-kinase in human A431 cell membrane by liquid scintillation counting Inhibition of phosphatidylinositol 4-kinase in human A431 cell membrane by liquid scintillation counting	[PMID: 1666120]
Vero	IC₅₀	11.5 μM Compound: R7	Inhibition of Shigella dysenteriae type 1 Shiga toxin A subunit in African green monkey Vero cells assessed as inhibition of Stx-induced cytotoxicity pre-treated with compound for 1 hr followed by Stx exposure for 24 hrs by neutral red uptake assay Inhibition of Shigella dysenteriae type 1 Shiga toxin A subunit in African green monkey Vero cells assessed as inhibition of Stx-induced cytotoxicity pre-treated with compound for 1 hr followed by Stx exposure for 24 hrs by neutral red uptake assay	[PMID: 27933947]
Vero	CC₅₀	136.5 μM Compound: R7	Cytotoxicity against African green monkey Vero cells incubated for 24 hrs by neutral red uptake assay Cytotoxicity against African green monkey Vero cells incubated for 24 hrs by neutral red uptake assay	[PMID: 27933947]

In Vitro

Guanine (1 h) inhibits Shigella dysenteriae type 1 Shiga toxin A subunit in African green monkey Vero cells, with IC₅₀ of 11.5 μM^[2].
Guanine (24 h) shows cytotoxicity against African green monkey Vero cells, with CC₅₀ of 136.5 μM^[2].
Guanine (10 μM, 16-40 h) partially antagonizes the cytostatic effect of Mycophenolic acid (HY-B0421) on LAN5 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Guanine (200–600 nmol, i.t., 5 min before behavioural evaluation) produces dose-dependent antinociceptive effects in the tail-flick, hot-plate, intraplantar (i.pl.) Glutamate, i.pl. Capsaicin (HY-10448) and Acetic acid (HY-Y0319) pain models^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Tail-flick, hot-plate, intraplantar (i.pl.) glutamate, i.pl. capsaicin and acetic acid pain mice models^[4]
Dosage:	200, 400, 600 nmol
Administration:	Intrathecal (i.t.), 5 min before behavioural evaluation
Result:	Produced antinociception against the i.pl. glutamate, i.pl. capsaicin, tail-flick, and i.p. acetic acid induced pain. Showed mean ID₅₀ of 84, 107, 48 nmol for capsaicin, glutamate, and acetic acid pain, respectively. Showed maximal inhibitions of 58%, 48%, 56% for capsaicin, glutamate, and acetic acid pain, respectively.

Molecular Weight

151.13

Formula

C₅H₅N₅O

CAS No.

73-40-5

Appearance

Solid

Color

White to off-white

SMILES

O=C1N=C(N)NC2=C1NC=N2

Structure Classification

Others

Initial Source

Microorganisms

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

0.5 M NaOH : 10 mg/mL (66.17 mM; ultrasonic and adjust pH to 12 with NaOH)

DMSO : 0.15 mg/mL (0.99 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.6169 mL	33.0843 mL	66.1686 mL
5 mM	1.3234 mL	6.6169 mL	13.2337 mL
10 mM	0.6617 mL	3.3084 mL	6.6169 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (249 KB) HNMR (232 KB)

Handling Instructions (2659 KB)

References

[1]. Mojzeš P, et al. Guanine, a high-capacity and rapid-turnover nitrogen reserve in microalgal cells. Proc Natl Acad Sci U S A. 2020 Dec 22;117(51):32722-32730. [Content Brief]

[2]. Chauhan V, et al. In Silico Discovery and Validation of Amide Based Small Molecule Targeting the Enzymatic Site of Shiga Toxin. J Med Chem. 2016 Dec 8;59(23):10763-10773. [Content Brief]

[3]. Messina E, et al. Guanine nucleotide depletion triggers cell cycle arrest and apoptosis in human neuroblastoma cell lines. Int J Cancer. 2004 Mar 1;108(6):812-7. [Content Brief]

[4]. de Oliveira ED, et al. Mechanisms involved in the antinociception induced by spinal administration of inosine or guanine in mice. Eur J Pharmacol. 2016 Feb 5;772:71-82. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

0.5 M NaOH	1 mM	6.6169 mL	33.0843 mL	66.1686 mL	165.4216 mL
	5 mM	1.3234 mL	6.6169 mL	13.2337 mL	33.0843 mL
	10 mM	0.6617 mL	3.3084 mL	6.6169 mL	16.5422 mL
	15 mM	0.4411 mL	2.2056 mL	4.4112 mL	11.0281 mL
	20 mM	0.3308 mL	1.6542 mL	3.3084 mL	8.2711 mL
	25 mM	0.2647 mL	1.3234 mL	2.6467 mL	6.6169 mL
	30 mM	0.2206 mL	1.1028 mL	2.2056 mL	5.5141 mL
	40 mM	0.1654 mL	0.8271 mL	1.6542 mL	4.1355 mL
	50 mM	0.1323 mL	0.6617 mL	1.3234 mL	3.3084 mL
	60 mM	0.1103 mL	0.5514 mL	1.1028 mL	2.7570 mL

Guanine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Guanine73-40-5DNA/RNA SynthesisEndogenous MetaboliteDNARNAnucleic acidspurinederivativeN poolInhibitorinhibitorinhibit

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Guanine

Customer Review

Other Forms of Guanine:

Guanine Related Antibodies

3 Publications Citing Use of MCE Guanine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Guanine Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Guanine Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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