IRAK-1-4 Inhibitor I (Synonyms: IRAK-1/4 Inhibitor I)

Name: IRAK-1-4 Inhibitor I
Brand: MedChemExpress
SKU: HY-13329
Rating: 5.0 (10 reviews)

Cat. No.: HY-13329 Purity: 99.58%: FAQs
Data Sheet SDS COA Handling Instructions

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IRAK-1-4 Inhibitor I is an inhibitor of interleukin-1 receptor-associated kinase 1/4 (IRAK 1/4) with IC₅₀s of 0.2 μM and 0.3 μM, respectively.

For research use only. We do not sell to patients.

IRAK-1-4 Inhibitor I Chemical Structure

CAS No. : 509093-47-4

Size	Price	Stock	Quantity
5 mg	USD 107	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 146	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 300	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 480	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 690	In-stock	Estimated Time of Arrival: December 31
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500 mg		Get quote

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Based on 10 publication(s) in Google Scholar

8 Publications Citing Use of MCE IRAK-1-4 Inhibitor I

: IRAK-1-4 Inhibitor I purchased from MedChemExpress. Usage Cited in: Oncogene. 2020 Sep;39(36):5888-5901. [Abstract]; The indicated cells are treated with IRAK1/4 inhibitor for 48 h. Nucleoplasm and chromatin-bound fractions are purified and immunoblots of the indicated antibodies are analyzed. Upon IRAK1/4 inhibitor treatment, while both chromatin-bound and free nucleoplasmic MLL are proportionally increased in the parental cells, only nucleoplasmic MLL is increased in the resistant cells.

: IRAK-1-4 Inhibitor I purchased from MedChemExpress. Usage Cited in: Antibiotics. 2020 Jan 17;9(1):33. [Abstract]; Huh7.5.1 cells are infected with J399EM at an MOI of 0.1, and then treated with different concentrations of IRAK-1/4 inhibitor I for 72 h. The expression level of the HCV core protein is determined by western blotting. The IRAK-1/4 inhibitor I is dissolved in dimethyl sulfoxide (DMSO) and the DMSO without IRAK-1/4 inhibitor I is used as a negative control.

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Biological Activity
Protocol
Purity & Documentation
References
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Description

IRAK-1-4 Inhibitor I is an inhibitor of interleukin-1 receptor-associated kinase 1/4 (IRAK 1/4) with IC₅₀s of 0.2 μM and 0.3 μM, respectively.

IC₅₀ & Target

IC50: 0.2 μM (IRAK-4), 0.3 μM (IRAK-1)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	ED₅₀	> 30 μM Compound: 46	Antiproliferative activity against human HeLa cell line after 72 hrs Antiproliferative activity against human HeLa cell line after 72 hrs	[PMID: 16563752]

In Vitro

IRAK-1-4 Inhibitor I has IC₅₀ greater than the highest concentration tested (10 μM) against a panel of 27 other kinases, including the most closely homologous (outside of the IRAK family) Lck and pp60^SRC. Additionally, IRAK-1-4 Inhibitor I does not show any signs of cytotoxicity in a 72 h proliferation assay in HeLa cells (ED₅₀>30 μM). Significant inhibition of IRAK-1 is observed with IRAK-1-4 Inhibitor I (IRAK-1 IC₅₀=0.3 μM)^[1]. IRAK-1/4 inhibitor eliminates the LPS-induced increases in Bcl10, NF-κB, and IL-8. IRAK-1/4 mediates LPS-induced IL-8 activation and functions upstream of Bcl10. The LPS-induced increase in Bcl10 declines by 73% (from 5.18±0.22 to 2.36±0.08 ng/mL), and the IL-8 response decline by 60% (from 2.64±0.31 to 1.14±0.08 ng/mL)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

395.41

Formula

C₂₀H₂₁N₅O₄

CAS No.

509093-47-4

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=CC=CC([N+]([O-])=O)=C1)NC2=NC3=CC=CC=C3N2CCN4CCOCC4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMF : 25 mg/mL (63.23 mM; ultrasonic and warming and heat to 60°C)

DMSO : 5 mg/mL (12.65 mM; ultrasonic and adjust pH to 3 with HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : < 1 mg/mL (insoluble)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5290 mL	12.6451 mL	25.2902 mL
5 mM	0.5058 mL	2.5290 mL	5.0580 mL
10 mM	0.2529 mL	1.2645 mL	2.5290 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.88%

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Data Sheet (277 KB) SDS (420 KB)

COA (252 KB) LCMS (136 KB)

Handling Instructions (2659 KB)

References

[1]. Powers JP, et al. Discovery and initial SAR of inhibitors of interleukin-1 receptor-associated kinase-4. Bioorg Med Chem Lett. 2006 Jun 1;16(11):2842-2845. [Content Brief]

[2]. Bhattacharyya S, et al. Bcl10 mediates LPS-induced activation of NF-kappaB and IL-8 in human intestinal epithelial cells. Am J Physiol Gastrointest Liver Physiol. 2007 Aug;293(2):G429-37. [Content Brief]

Cell Assay ^[2]	NCM460 cells, grown in 24-well plates, are incubated with 50 μM IRAK-1/4 inhibitor for 2 h. After 2 h, the media are changed, and new media with or without LPS (10 ng/mL) added. Treatment is terminated at 6 h, and spent media and cells are collected for IL-8 and other assays^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Powers JP, et al. Discovery and initial SAR of inhibitors of interleukin-1 receptor-associated kinase-4. Bioorg Med Chem Lett. 2006 Jun 1;16(11):2842-2845. [Content Brief] [2]. Bhattacharyya S, et al. Bcl10 mediates LPS-induced activation of NF-kappaB and IL-8 in human intestinal epithelial cells. Am J Physiol Gastrointest Liver Physiol. 2007 Aug;293(2):G429-37. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / DMF	1 mM	2.5290 mL	12.6451 mL	25.2902 mL	63.2255 mL
	5 mM	0.5058 mL	2.5290 mL	5.0580 mL	12.6451 mL
	10 mM	0.2529 mL	1.2645 mL	2.5290 mL	6.3226 mL
DMF	15 mM	0.1686 mL	0.8430 mL	1.6860 mL	4.2150 mL
	20 mM	0.1265 mL	0.6323 mL	1.2645 mL	3.1613 mL
	25 mM	0.1012 mL	0.5058 mL	1.0116 mL	2.5290 mL
	30 mM	0.0843 mL	0.4215 mL	0.8430 mL	2.1075 mL
	40 mM	0.0632 mL	0.3161 mL	0.6323 mL	1.5806 mL
	50 mM	0.0506 mL	0.2529 mL	0.5058 mL	1.2645 mL
	60 mM	0.0422 mL	0.2108 mL	0.4215 mL	1.0538 mL

IRAK-1-4 Inhibitor I Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IRAK-1-4 Inhibitor I509093-47-4IRAK-1/4 Inhibitor IIRAKInterleukin-1 receptor associated kinaseIL-1R associated kinaseInhibitorinhibitorinhibit

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IRAK-1-4 Inhibitor I (Synonyms: IRAK-1/4 Inhibitor I)

Customer Review

IRAK-1-4 Inhibitor I Related Antibodies

8 Publications Citing Use of MCE IRAK-1-4 Inhibitor I

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Biological Activity

Protocol

Purity & Documentation

References

Customer Review

IRAK-1-4 Inhibitor I Related Antibodies

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Complete Stock Solution Preparation Table

IRAK-1-4 Inhibitor I Related Classifications

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