Levomefolic acid  (Synonyms: L-5-MTHF)

Levomefolic acid (L-5-MTHF) is an orally active, brain-penetrant natural active form of folic acid and is one of the most widely used folic acid food supplements^[1][2].

Levomefolic acid (L-5-MTHF) shows interaction with residues Glu406, Ile 418, Lys417 and Tyr453, of which Lys 417 and Tyr 453 play a strong physiological role in the interaction between S1 and ACE and may act as a potential inhibitor of the S1:ACE2 interaction of the SARS-CoV-2 virus^[1].
Levomefolic acid (L-5-MTHF) (50 nM, 72 h) increases intracellular folate metabolite activity by 7-fold better than folic acid by 2-fold in human lymphoblastoid cell line (LCL), and is a good folic acid supplement^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

459.46

C₂₀H₂₅N₇O₆

31690-09-2

Solid

Light yellow to yellow

O=C(O)CC[C@@H](C(O)=O)NC(C1=CC=C(NC[C@@H]2N(C)C3=C(NC(N)=NC3=O)NC2)C=C1)=O

Room temperature in continental US; may vary elsewhere.

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Manisha Prajapat, et al. Virtual screening and molecular dynamics study of approved drugs as inhibitors of spike protein S1 domain and ACE2 interaction in SARS-CoV-2. J Mol Graph Model. 2020 Dec;101:107716.  [Content Brief]

  [2]. Maša Vidmar Golja, et al. Simultaneous quantification of intracellular concentrations of clinically important metabolites of folate-homocysteine cycle by LC-MS/MS. Anal Biochem. 2020 Sep 15;605:113830.  [Content Brief]