N6-(4-Hydroxybenzyl)adenosine (Synonyms: Para-topolin riboside)

Name: N6-(4-Hydroxybenzyl)adenosine
Brand: MedChemExpress
SKU: HY-18775
Rating: 4.5 (1 reviews)

Cat. No.: HY-18775 Purity: 99.96%: FAQs
Data Sheet SDS COA Handling Instructions

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N6-(4-Hydroxybenzyl)adenosine is a inhibitor of platelet aggregation induced in vitro by collagen and their activity range was demonstrated (IC50: 6.

For research use only. We do not sell to patients.

N6-(4-Hydroxybenzyl)adenosine Chemical Structure

CAS No. : 110505-75-4

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 57	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 57	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 52	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 84	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 288	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 480	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE N6-(4-Hydroxybenzyl)adenosine

•University of Cambridge. 2023 Feb.

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Biological Activity
Purity & Documentation
References
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Description

N6-(4-Hydroxybenzyl)adenosine is a inhibitor of platelet aggregation induced in vitro by collagen and their activity range was demonstrated (IC50: 6.77-141 μM). IC50 value: 6.77-141 μM Target: P2Y12receptor Anti-aggregation activity of N6-(4-Hydroxybenzyl)adenosine could involve an interaction with the P2Y12receptor binding site.

Cellular Effect

Cell Line	Type	Value	Description	References
PC-12	EC₅₀	0.037 μM Compound: 2	Prevention of serum deprivation-induced PC12 cell apoptosis by MTT assay Prevention of serum deprivation-induced PC12 cell apoptosis by MTT assay	[PMID: 17381154]
Platelet	IC₅₀	6.77 μM Compound: 2e	Inhibition of collagen-induced human platelet aggregation after 3 mins by light transmission aggregometry Inhibition of collagen-induced human platelet aggregation after 3 mins by light transmission aggregometry	[PMID: 25467153]

Molecular Weight

373.36

Formula

C₁₇H₁₉N₅O₅

CAS No.

110505-75-4

Appearance

Solid

Color

Light yellow to yellow

SMILES

OC[C@@H]1[C@H]([C@H]([C@H](N2C=NC3=C2N=CN=C3NCC4=CC=C(O)C=C4)O1)O)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (267.84 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 1 mg/mL (2.68 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6784 mL	13.3919 mL	26.7838 mL
5 mM	0.5357 mL	2.6784 mL	5.3568 mL
10 mM	0.2678 mL	1.3392 mL	2.6784 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (249 KB) LCMS (92 KB)

Handling Instructions (2659 KB)

References

[1]. Vistoli G, et al. Naturally occurring N(6)-substituted adenosines (cytokinin ribosides) are in vitro inhibitors of platelet aggregation: an in silico evaluation of their interaction with the P2Y(12) receptor. Bioorg Med Chem Lett. 2014 Dec 15;24(24):5652-5655. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.6784 mL	13.3919 mL	26.7838 mL	66.9595 mL
DMSO	5 mM	0.5357 mL	2.6784 mL	5.3568 mL	13.3919 mL
	10 mM	0.2678 mL	1.3392 mL	2.6784 mL	6.6960 mL
	15 mM	0.1786 mL	0.8928 mL	1.7856 mL	4.4640 mL
	20 mM	0.1339 mL	0.6696 mL	1.3392 mL	3.3480 mL
	25 mM	0.1071 mL	0.5357 mL	1.0714 mL	2.6784 mL
	30 mM	0.0893 mL	0.4464 mL	0.8928 mL	2.2320 mL
	40 mM	0.0670 mL	0.3348 mL	0.6696 mL	1.6740 mL
	50 mM	0.0536 mL	0.2678 mL	0.5357 mL	1.3392 mL
	60 mM	0.0446 mL	0.2232 mL	0.4464 mL	1.1160 mL
	80 mM	0.0335 mL	0.1674 mL	0.3348 mL	0.8370 mL
	100 mM	0.0268 mL	0.1339 mL	0.2678 mL	0.6696 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

N6-(4-Hydroxybenzyl)adenosine Related Classifications

Cardiovascular Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N6-(4-Hydroxybenzyl)adenosine110505-75-4Para-topolin ribosideP2Y ReceptorInhibitorinhibitorinhibit

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N6-(4-Hydroxybenzyl)adenosine (Synonyms: Para-topolin riboside)

Customer Review

N6-(4-Hydroxybenzyl)adenosine Related Antibodies

1 Publications Citing Use of MCE N6-(4-Hydroxybenzyl)adenosine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All P2Y Receptor Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

N6-(4-Hydroxybenzyl)adenosine Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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