2. Membrane Transporter/Ion Channel
  3. Potassium Channel
  4. NS309

NS309 is a potent and selective activator of the Ca2+-activated SK/IK potassium channels, but displays no activity at BK channels.

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NS309 Chemical Structure

NS309 Chemical Structure

CAS No. : 18711-16-5

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 73 In-stock
Solution
10 mM * 1 mL in DMSO USD 73 In-stock
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5 mg USD 66 In-stock
10 mg USD 79 In-stock
50 mg USD 277 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

NS309 Related Antibodies

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Description

NS309 is a potent and selective activator of the Ca2+-activated SK/IK potassium channels, but displays no activity at BK channels[1][2][3][4].

IC50 & Target

EC50: 0.62 µM (SK2)[1], 0.3 μM (SK3)[2], ∼20 nM (KCa3.1/SK4)[3], ∼600 nM (KCa2/SK channels)[3], 10 nM (hIK)[4]
In Vitro

NS309 (40?nM) activates both hSK3 and hIK channels, with the largest effect on hIK channels (8.5-fold increase in current compares to 1.9-fold increase in current for hSK3 channels)[2].
NS309 (10 μM) significantly increases the whole cell SK currents and hyperpolarized detrusor smooth muscle (DSM) cells resting membrane potential[5].
NS309 inhibits the spontaneous phasic contraction amplitude, force, frequency, duration and tone of isolated DSM strips in a concentration-dependent manner[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NS309 Related Antibodies
In Vivo

NS309 (2 mg/kg; i.p.) protects against SCI/R in rabbits[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adult New Zealand white rabbits (2.5-3.0 kg)[6]
Dosage: 2 mg/kg
Administration: Intraperitoneal injection
Result: Significantly improved neurological outcome of SCI/R challenged rabbits.
Molecular Weight

231.04

Formula

C8H4Cl2N2O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1NC2=C(C=CC(Cl)=C2Cl)/C1=N/O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (432.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.3283 mL 21.6413 mL 43.2826 mL
5 mM 0.8657 mL 4.3283 mL 8.6565 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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  • Dilution Calculator

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This equation is commonly abbreviated as: C1V1 = C2V2

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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.3283 mL 21.6413 mL 43.2825 mL 108.2064 mL
5 mM 0.8657 mL 4.3283 mL 8.6565 mL 21.6413 mL
10 mM 0.4328 mL 2.1641 mL 4.3283 mL 10.8206 mL
15 mM 0.2886 mL 1.4428 mL 2.8855 mL 7.2138 mL
20 mM 0.2164 mL 1.0821 mL 2.1641 mL 5.4103 mL
25 mM 0.1731 mL 0.8657 mL 1.7313 mL 4.3283 mL
30 mM 0.1443 mL 0.7214 mL 1.4428 mL 3.6069 mL
40 mM 0.1082 mL 0.5410 mL 1.0821 mL 2.7052 mL
50 mM 0.0866 mL 0.4328 mL 0.8657 mL 2.1641 mL
60 mM 0.0721 mL 0.3607 mL 0.7214 mL 1.8034 mL
80 mM 0.0541 mL 0.2705 mL 0.5410 mL 1.3526 mL
100 mM 0.0433 mL 0.2164 mL 0.4328 mL 1.0821 mL
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NS309 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NS30918711-16-5NS 309NS-309Potassium ChannelKcsAoveractivebladderOABneuronalhyperexcitabilityspinalcordischemia-reperfusioninjurySCI/RerectilefunctionInhibitorinhibitorinhibit

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