OVA Peptide(257-264)

Name: OVA Peptide(257-264)
Brand: MedChemExpress
SKU: HY-P1489
Rating: 5.0 (4 reviews)

Cat. No.: HY-P1489 Purity: 99.68%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

OVA Peptide(257-264) is a class I (Kb)-restricted peptide epitope of OVA, an octameric peptide can be from ovalbumin presented by the class I MHC molecule, H-2Kb.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

OVA Peptide(257-264) Chemical Structure

CAS No. : 138831-86-4

Size	Price	Stock	Quantity
1 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 170	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 270	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of OVA Peptide(257-264):

OVA Peptide(257-264) TFA In-stock
OVA Peptide(257-264) acetate salt Get quote

4 Publications Citing Use of MCE OVA Peptide(257-264)

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

OVA Peptide(257-264) is a class I (Kb)-restricted peptide epitope of OVA, an octameric peptide can be from ovalbumin presented by the class I MHC molecule, H-2Kb.

In Vitro

TAP1-I- and C57BL/6 macrophages may process Crl-OVA and full-length OVA in different cellular compartments and that the protein context of the OVA Peptide(257-264) epitope influences the extent of TAP-independent processing for MHC class I presentation. OVA Peptide(257-264) epitope is presented with a differential dependence on the TAP transporter depending on the protein context of the OVA epitope: OVA Peptide(257-264) contained within the MBPCrl-OVA or Crl-OVA bacterial fusion proteins is presented with little dependence on the TAP transporter, while OVA Peptide(257-264) contained within full-length ovalbumin is largely dependent on the TAP transporter, regardless of whether recombinant OVA is expressed in bacteria or the native protein is coupled to polystyrene beads^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

963.13

Formula

C₄₅H₇₄N₁₀O₁₃

CAS No.

138831-86-4

Appearance

Solid

Color

White to off-white

Sequence

Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu

Sequence Shortening

SIINFEKL

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 40 mg/mL (41.53 mM; ultrasonic and adjust pH to 3 with 1 M HCL; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

0.1% TFA in Water : 1.83 mg/mL (1.90 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.0383 mL	5.1914 mL	10.3828 mL
5 mM	0.2077 mL	1.0383 mL	2.0766 mL
10 mM	0.1038 mL	0.5191 mL	1.0383 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.68%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (248 KB) RP-HPLC (207 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Wick MJ, et al. Major histocompatibility complex class I presentation of ovalbumin peptide 257-264 from exogenous sources: protein context influences the degree of TAP-independent presentation. Eur J Immunol. 1996 Nov;26(11):2790-9. [Content Brief]

Cell Assay

TAPl^-/- or C57BL/6 macrophages are co-incubated with either bacteria or polystyrene beads containing the 257-264 epitope from ovalbumin [OVA Peptide(257-264)], which binds the mouse class I molecule Kb. The source of the OVA(257-264) epitope is either the Crl-OVA(257-264) (Crl-OVA) fusion protein, the maltose binding protein (MBP)-Crl-OVA fusion protein, native OVA or bacterial recombinant OVA (rOVA); Crl-OVA, MBP-Crl-OVA and rOVA are each expressed in bacteria, and Crl-OVA and MBP-Crl-OVA purified from bacterial lysates and native egg OVA are coated onto polystyrene beads^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Wick MJ, et al. Major histocompatibility complex class I presentation of ovalbumin peptide 257-264 from exogenous sources: protein context influences the degree of TAP-independent presentation. Eur J Immunol. 1996 Nov;26(11):2790-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

0.1% TFA in Water / DMSO	1 mM	1.0383 mL	5.1914 mL	10.3828 mL	25.9570 mL
DMSO	5 mM	0.2077 mL	1.0383 mL	2.0766 mL	5.1914 mL
	10 mM	0.1038 mL	0.5191 mL	1.0383 mL	2.5957 mL
	15 mM	0.0692 mL	0.3461 mL	0.6922 mL	1.7305 mL
	20 mM	0.0519 mL	0.2596 mL	0.5191 mL	1.2979 mL
	25 mM	0.0415 mL	0.2077 mL	0.4153 mL	1.0383 mL
	30 mM	0.0346 mL	0.1730 mL	0.3461 mL	0.8652 mL
	40 mM	0.0260 mL	0.1298 mL	0.2596 mL	0.6489 mL