2. GPCR/G Protein Neuronal Signaling
  3. mAChR
  4. Otilonium bromide

Otilonium bromide  (Synonyms: Octylonium bromide; SP63)

Cat. No.: HY-B0499A Purity: 99.69%
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Otilonium bromide (OB) is an orally active mAChR inhibitor and smooth muscle relaxant which can interfere with the mobilization of calcium in intestinal smooth muscle, OB can be used for research of irritable bowel syndrome.

For research use only. We do not sell to patients.

Otilonium bromide Chemical Structure

Otilonium bromide Chemical Structure

CAS No. : 26095-59-0

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Based on 2 publication(s) in Google Scholar

Other Forms of Otilonium bromide:

Otilonium bromide Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

Otilonium bromide (OB) is an orally active mAChR inhibitor and smooth muscle relaxant which can interfere with the mobilization of calcium in intestinal smooth muscle, OB can be used for research of irritable bowel syndrome[1][2][3].

In Vitro

Otilonium bromide (10 nM-100 μM) inhibits acetylcholine-induced human colonic crypt calcium signals in a dose dependent manner with an IC50 of 880 nM[2].
Otilonium bromide (30 µM) inhibits Ca2+ uptake in bovine chromaffin cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Otilonium bromide Related Antibodies
Clinical Trial
Molecular Weight

563.57

Formula

C29H43BrN2O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(OCC[N+](CC)(CC)C)C1=CC=C(NC(C2=CC=CC=C2OCCCCCCCC)=O)C=C1.[Br-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (177.44 mM)

DMSO : 50 mg/mL (88.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7744 mL 8.8720 mL 17.7440 mL
5 mM 0.3549 mL 1.7744 mL 3.5488 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (4.44 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (4.44 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 1.7744 mL 8.8720 mL 17.7440 mL 44.3601 mL
5 mM 0.3549 mL 1.7744 mL 3.5488 mL 8.8720 mL
10 mM 0.1774 mL 0.8872 mL 1.7744 mL 4.4360 mL
15 mM 0.1183 mL 0.5915 mL 1.1829 mL 2.9573 mL
20 mM 0.0887 mL 0.4436 mL 0.8872 mL 2.2180 mL
25 mM 0.0710 mL 0.3549 mL 0.7098 mL 1.7744 mL
30 mM 0.0591 mL 0.2957 mL 0.5915 mL 1.4787 mL
40 mM 0.0444 mL 0.2218 mL 0.4436 mL 1.1090 mL
50 mM 0.0355 mL 0.1774 mL 0.3549 mL 0.8872 mL
60 mM 0.0296 mL 0.1479 mL 0.2957 mL 0.7393 mL
80 mM 0.0222 mL 0.1109 mL 0.2218 mL 0.5545 mL
H2O 100 mM 0.0177 mL 0.0887 mL 0.1774 mL 0.4436 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Otilonium bromide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Otilonium26095-59-0Octylonium SP63SP63SP 63SP-63mAChRMuscarinic acetylcholine receptorIrritable bowel syndromeSpasmolytic agentChromaffin cellsInhibitorinhibitorinhibit

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