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  5. PrP (106-126)

PrP (106-126)  (Synonyms: Prion protein (106-126))

Cat. No.: HY-P0305
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PrP (106-126) is a peptide corresponding to the prion protein (PrP) amyloidogenic region, and its biochemical properties resemble the infectious form of prion protein.

For research use only. We do not sell to patients.

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PrP (106-126) Chemical Structure

PrP (106-126) Chemical Structure

CAS No. : 148439-49-0

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Based on 1 publication(s) in Google Scholar

PrP (106-126) Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

PrP (106-126) is a peptide corresponding to the prion protein (PrP) amyloidogenic region, and its biochemical properties resemble the infectious form of prion protein.

In Vitro

PrP (106-126) (100 μM) induces mTOR phosphorylation over time in N2a cells. PrP (106-126)-treated cells show significantly increased ROS production in comparison with that of PBS-treated control cells. Knockdown of PRAS40 enhances PrP (106-126)-induced apoptosis. PRAS40 alleviates PrP (106-126)-induced neuronal apoptosis via mTOR-AKT activation[1]. PrP (106-126) interacts selectively with porcine brain endothelial cells (PBEC) via their luminal side, and causes cumulative cell death, as shown by lactate dehydrogenase release, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction, Caspase 3 induction and direct cell counting. In addition, PrP (106-126), but not its corresponding scrambled peptide, produces a 50% reduction of the trans-endothelial electrical resistance, while the PBEC maintained confluency[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PrP (106-126) Related Antibodies
Molecular Weight

1912.30

Formula

C80H138N26O24S2
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Lys-Thr-Asn-Met-Lys-His-Met-Ala-Gly-Ala-Ala-Ala-Ala-Gly-Ala-Val-Val-Gly-Gly-Leu-Gly
Sequence Shortening

KTNMKHMAGAAAAGAVVGGLG
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 1 mg/mL (0.52 mM; Need ultrasonic and warming)

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (52.29 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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Working solution concentration: mg/mL
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References
Cell Assay
[2]

The toxicity of PrP peptides is investigated on monolayers of PBEC. 100 000 PBEC/cm2 are seeded on 96 [for 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)] or 24-well plates [for lactate dehydrogenase (LDH)] pre-coated with rat tail collagen (27 μg/mL). The cell monolayers are treated with either PrP 106-126 wt or scr peptides. The PrP peptides are dissolved in double distilled water and then diluted in assay medium or in 5% newborn calf serum in Dulbecco-modified Earl's medium without phenol red. Treatment duration with the PrP peptides varies from 24 h to 48 h and the peptide concentration is set to 100 μM at which it exerts optimal effects as shown in a dose-dependent experiment. This concentration is also chosen as similar concentrations of PrP 106-126 found to exert in-vitro effects on astrocytes, neurons, microglia and leukocytes. The MTT assay, which measures mitochondrial metabolism, is based on the conversion of the water-soluble MTT dye [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] to an insoluble purple formazan. This formazan is then solubilized in pure dimethylsulfoxide, and its concentration determined by optical density (550/630 nm). The LDH cell death assay is done using the CytoTox 96® Assay kit. The released LDH in culture supernatants is measured with a 30-min coupled enzymatic assay which results in the conversion of a tetrazolium salt, 2-p-iodophenyl-3-p-nitro-phenyl-5-phenyl tetrazolium chloride (INT) into a red formazan product. The LDH levels are measured at the end of each treatment period. Thus, 50 μL of the medium is removed at the end of the treatment with PrP peptides and transferred to a new 96-well plate to which the other compounds are added. The plates are analyzed using an Elisa reader with the 490 nm filter.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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PrP (106-126) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PrP (106-126)148439-49-0Prion protein (106-126)OthersInhibitorinhibitorinhibit

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