Promethazine hydrochloride

Name: Promethazine hydrochloride
Brand: MedChemExpress
SKU: HY-B0781
Rating: 4.5 (1 reviews)

Cat. No.: HY-B0781 Purity: 99.20%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Promethazine hydrochloride is an orally active phenothiazine derivative with antihistaminic (H1), sedative, antiemetic, anticholinergic, and antimotion sickness properties. Promethazine hydrochloride is a potent H1 receptor antagonist and a mAChR antagonist. It also has a certain affinity for 5-HT2A and 5-HT2C receptors.

For research use only. We do not sell to patients.

Promethazine hydrochloride Chemical Structure

CAS No. : 58-33-3

Size	Price	Stock	Quantity
500 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
1 g	USD 35	In-stock	Estimated Time of Arrival: December 31
5 g	USD 50	In-stock	Estimated Time of Arrival: December 31
10 g		Get quote
50 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Promethazine hydrochloride:

Promethazine-d₄ hydrochloride Get quote
Promethazine Get quote
Promethazine hydrochloride (Standard) Get quote

1 Publications Citing Use of MCE Promethazine hydrochloride

•PLoS Negl Trop Dis. 2019 Aug 20;13(8):e0007681. [Abstract]

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Histamine Receptor Isoform Specific Products:

View All Isoforms

H₁ Receptor H₂ Receptor H₃ Receptor H₄ Receptor Histamine Receptor

View All mAChR Isoform Specific Products:

View All Isoforms

mAChR1 mAChR2 mAChR3 mAChR4 mAChR5

View All Adrenergic Receptor Isoform Specific Products:

View All Isoforms

α adrenergic receptor β adrenergic receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

H₁ Receptor

In Vitro

Promethazine hydrochloride (1.25-10 μM, 3 days) inhibits adipocyte formation in a dose-dependent manner^[1].
Promethazine hydrochloride (10 μM, 0-12 days) decreases the expression of peroxisome proliferator activated receptor γ (PPARG) and reduces the phosphorylation level of CREB in PDGFRα⁺ cells^[1].
Promethazine hydrochloride (10-1000 μM, 1-24 h) has cytotoxic at concentrations greater than 100 μM in L929 lung fibroblast cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Promethazine hydrochloride (0.05-0.1 mg/mL, p.o., 4 weeks) possesses inhibitory effect on ectopic fat cell formation in skeletal muscle in a mouse achilles tendon rupture model^[1].
Promethazine hydrochloride (2.4-9.6 mg/kg, p.o.) has no effect on the development of femoral osteoporosis and retarded normal femoral expansion in the adult castrate male rats^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

320.88

Formula

C₁₇H₂₁ClN₂S

CAS No.

58-33-3

Appearance

Solid

Color

White to off-white

SMILES

CC(N(C)C)CN1C(C=CC=C2)=C2SC3=CC=CC=C13.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (311.64 mM; Need ultrasonic)

DMSO : 50 mg/mL (155.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1164 mL	15.5821 mL	31.1643 mL
5 mM	0.6233 mL	3.1164 mL	6.2329 mL
10 mM	0.3116 mL	1.5582 mL	3.1164 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.79 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.79 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.79 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 120 mg/mL (373.97 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.20%

Select Batch:

Data Sheet (277 KB) SDS (538 KB)

COA (251 KB) HNMR (271 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Kasai T, et al. Promethazine Hydrochloride Inhibits Ectopic Fat Cell Formation in Skeletal Muscle. Am J Pathol. 2017 Dec;187(12):2627-2634. [Content Brief]

[2]. Fiorella D, et al. The role of the 5-HT2A and 5-HT2C receptors in the stimulus effects of hallucinogenic drugs. I: Antagonist correlation analysis. Psychopharmacology (Berl). 1995 Oct;121(3):347-56. [Content Brief]

[3]. McDonough JA, et al. Microcapsule-gel formulation of promethazine HCl for controlled nasal delivery: a motion sickness medication. J Microencapsul. 2007 Mar;24(2):109-16. [Content Brief]

[4]. Wink CS, et al. Effects of promethazine HCl on osteoporotic femora of adult castrated male rats. Acta Anat (Basel). [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	3.1164 mL	15.5821 mL	31.1643 mL	77.9107 mL
	5 mM	0.6233 mL	3.1164 mL	6.2329 mL	15.5821 mL
	10 mM	0.3116 mL	1.5582 mL	3.1164 mL	7.7911 mL
	15 mM	0.2078 mL	1.0388 mL	2.0776 mL	5.1940 mL
	20 mM	0.1558 mL	0.7791 mL	1.5582 mL	3.8955 mL
	25 mM	0.1247 mL	0.6233 mL	1.2466 mL	3.1164 mL
	30 mM	0.1039 mL	0.5194 mL	1.0388 mL	2.5970 mL
	40 mM	0.0779 mL	0.3896 mL	0.7791 mL	1.9478 mL
	50 mM	0.0623 mL	0.3116 mL	0.6233 mL	1.5582 mL
	60 mM	0.0519 mL	0.2597 mL	0.5194 mL	1.2985 mL
	80 mM	0.0390 mL	0.1948 mL	0.3896 mL	0.9739 mL
	100 mM	0.0312 mL	0.1558 mL	0.3116 mL	0.7791 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.