Ridinilazole (Synonyms: SMT19969)

Cat. No.: HY-16753 Purity: 99.02%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Ridinilazole is a novel antibacterial with MICs range of 0.06-0.25 µg/mL (MIC₉₀=8 µg/mL) against C. difficile.

For research use only. We do not sell to patients.

Ridinilazole Chemical Structure

CAS No. : 308362-25-6

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 220	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 220	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 95	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 330	In-stock	Estimated Time of Arrival: December 31
20 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 820	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1150	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Ridinilazole is a novel antibacterial with MICs range of 0.06-0.25 µg/mL (MIC₉₀=8 µg/mL) against C. difficile.

IC₅₀ & Target

MIC₉₀: 8 µg/mL (C. difficile)^[1]

In Vitro

Ridinilazole is a novel antibacterial that does not appear to act through the classical pathways associated with antibiotics, such as inhibition of cell wall, protein, lipid, RNA or DNA synthesis. Ridinilazole may impair cell division. Ridinilazole is bactericidal against C. difficile and exhibits a prolonged post-antibiotic effect. In susceptibility testing of 82 clinical isolates of C. difficile (including ribotype 027), Ridinilazole displays potent growth inhibition and has lower MICs [MIC range, 0.06-0.25 µg/mL; MIC for 90% of the organisms (MIC90), 0.125 µg/mL] than Metronidazole (MIC range, 0.125-8 µg/mL; MIC₉₀, 8 µg/mL) or Vancomycin (MIC range, 0.5-4 µg/mL; MIC₉₀, 2 µg/mL). Similarly, Ridinilazole is found to be more potent than Metronidazole or Vancomycin at inhibiting the growth of 50 ribotype-defined C. difficile strains. The activity of Ridinilazole against specific C. difficile ribotypes (including ribotypes 001, 002, 005, 014, 027, 054 and 106) is similar, with an MIC range of 0.06–0.5 µg/mL and an MIC₉₀ of 0.125 µg/mL. In addition, Ridinilazole is more active against 11 ribotype 027 strains than either Metronidazole or Vancomycin^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

In a hamster model of CDI with a once-daily dosing regimen, Ridinilazole displays greater efficacy than Vancomycin both against non-epidemic and epidemic strains of C. difficile. Similar to the twice-daily dosing study, plasma levels of Ridinilazole are below the level of detection, whereas caecal Ridinilazole concentrations are well above the MIC, thus demonstrating the non-absorbable nature of Ridinilazole and minimal systemic exposure^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

388.42

Formula

C₂₄H₁₆N₆

CAS No.

308362-25-6

Appearance

Solid

Color

Light yellow to yellow

SMILES

C1(C2=CC=NC=C2)=NC3=CC(C4=CC=C5C(N=C(C6=CC=NC=C6)N5)=C4)=CC=C3N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (64.36 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5745 mL	12.8727 mL	25.7453 mL
5 mM	0.5149 mL	2.5745 mL	5.1491 mL
10 mM	0.2575 mL	1.2873 mL	2.5745 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.02%

Select Batch:

Data Sheet (271 KB) SDS (393 KB)

COA (245 KB) RP-HPLC (244 KB)

Handling Instructions (2659 KB)

References

[1]. Vickers RJ, et al. Ridinilazole: a novel therapy for Clostridium difficile infection. Int J Antimicrob Agents. 2016 Aug;48(2):137-43. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5745 mL	12.8727 mL	25.7453 mL	64.3633 mL
	5 mM	0.5149 mL	2.5745 mL	5.1491 mL	12.8727 mL
	10 mM	0.2575 mL	1.2873 mL	2.5745 mL	6.4363 mL
	15 mM	0.1716 mL	0.8582 mL	1.7164 mL	4.2909 mL
	20 mM	0.1287 mL	0.6436 mL	1.2873 mL	3.2182 mL
	25 mM	0.1030 mL	0.5149 mL	1.0298 mL	2.5745 mL
	30 mM	0.0858 mL	0.4291 mL	0.8582 mL	2.1454 mL
	40 mM	0.0644 mL	0.3218 mL	0.6436 mL	1.6091 mL
	50 mM	0.0515 mL	0.2575 mL	0.5149 mL	1.2873 mL
	60 mM	0.0429 mL	0.2145 mL	0.4291 mL	1.0727 mL

Ridinilazole Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ridinilazole308362-25-6SMT19969SMT 19969SMT-19969BacterialInhibitorinhibitorinhibit

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