2. Protein Tyrosine Kinase/RTK Metabolic Enzyme/Protease
  3. SHP2 Phosphatase
  4. SHP394

SHP394 is an orally active, selective and allosteric inhibitor of SHP2, with an IC50 of 23 nM.

For research use only. We do not sell to patients.

SHP394 Chemical Structure

SHP394 Chemical Structure

CAS No. : 2055757-40-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 715 In-stock
Solution
10 mM * 1 mL in DMSO USD 715 In-stock
Solid
5 mg USD 650 In-stock
10 mg USD 950 In-stock
25 mg USD 1900 In-stock
50 mg USD 3050 In-stock
100 mg USD 4850 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

SHP394 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE SHP394

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

SHP394 is an orally active, selective and allosteric inhibitor of SHP2, with an IC50 of 23 nM[1].

IC50 & Target

IC50: 23 nM (SHP2)[1]
Cellular Effect
Cell Line Type Value Description References
Detroit 562 IC50
1.38 μM
Compound: 1; SHP394
Antiproliferative activity against human Detroit 562 cells after 24 hrs by Celltiter-Glo assay
Antiproliferative activity against human Detroit 562 cells after 24 hrs by Celltiter-Glo assay
[PMID: 30688459]
KYSE-520 cell line IC50
0.297 μM
Compound: 1; SHP394
Antiproliferative activity against human KYSE520 cells added 24 hrs after cell plating and measured on day 5 by Celltiter-Glo assay
Antiproliferative activity against human KYSE520 cells added 24 hrs after cell plating and measured on day 5 by Celltiter-Glo assay
[PMID: 30688459]
In Vitro

SHP394 inhibits Caco-2 cells proliferation with the IC50 of 297 nM[1].
SHP394 exhibits antiproliferation activity against the Detroit-562 pharyngeal carcinoma cell line in vitro (IC50= 1.38 μM) [1].
SHP394 decreases p-ERK with an IC50 of 18 nM KYSE520 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SHP394 Related Antibodies
In Vivo

SHP394 (20-80 mg/kg; oral gavage; twice daily) dose-dependent reduces tumor volume[1].
SHP394 (80 mg/kg; oral gavage; twice daily) causes tumor 34% regression and reduces mouse host bodyweight after dosing for 14 days[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Six-week old female athymic NU/NU mice were inoculated subcutaneously with Detroit-562 pharyngeal carcinoma cells[1].
Dosage: 20, 40, and 80 mg/kg
Administration: Oral gavage; twice daily
Result: Demonstrated a clear dose-dependent reduction in tumor volume.
Molecular Weight

470.51

Formula

C20H25F3N6O2S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NC1=C(C(N(C)C(N2CCC3(CO[C@@H](C)[C@H]3N)CC2)=N1)=O)SC4=C(C(F)(F)F)N=CC=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 24 mg/mL (51.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1254 mL 10.6268 mL 21.2535 mL
5 mM 0.4251 mL 2.1254 mL 4.2507 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 6.25 mg/mL (13.28 mM); Clear solution

    This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 6.25 mg/mL (13.28 mM); Clear solution

    This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1254 mL 10.6268 mL 21.2535 mL 53.1338 mL
5 mM 0.4251 mL 2.1254 mL 4.2507 mL 10.6268 mL
10 mM 0.2125 mL 1.0627 mL 2.1254 mL 5.3134 mL
15 mM 0.1417 mL 0.7085 mL 1.4169 mL 3.5423 mL
20 mM 0.1063 mL 0.5313 mL 1.0627 mL 2.6567 mL
25 mM 0.0850 mL 0.4251 mL 0.8501 mL 2.1254 mL
30 mM 0.0708 mL 0.3542 mL 0.7085 mL 1.7711 mL
40 mM 0.0531 mL 0.2657 mL 0.5313 mL 1.3283 mL
50 mM 0.0425 mL 0.2125 mL 0.4251 mL 1.0627 mL
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SHP394 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SHP3942055757-40-7SHP 394SHP-394SHP2PhosphataseSrc homology phosphatase 2PTPN11allostericCaco-2Detroit-562pharyngealcarcinomap-ERKKYSE520Inhibitorinhibitorinhibit

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