2. Metabolic Enzyme/Protease Vitamin D Related/Nuclear Receptor
  3. Endogenous Metabolite VD/VDR
  4. Secalciferol

Secalciferol  (Synonyms: (24R)-24,25-Dihydroxyvitamin D3)

Cat. No.: HY-32343 Purity: 99.89%
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Secalciferol ((24R)-24,25-Dihydroxyvitamin D3) is the major active metabolite of Vitamin D. Secalciferol activates vitamin D receptor (VDR) with an EC50 value of 150 nM. Secalciferol is involved in a wide range of biological functions such as calcium homeostasis, cellular differentiation and proliferation processes, as well as other functions related to the immune system, which is promising for research of rickets, osteomalacia, hypercalcemia and autoimmune disorders.

For research use only. We do not sell to patients.

Secalciferol Chemical Structure

Secalciferol Chemical Structure

CAS No. : 55721-11-4

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1 mg USD 600 In-stock
5 mg USD 2640 In-stock
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Based on 4 publication(s) in Google Scholar

Other Forms of Secalciferol:

Secalciferol Related Antibodies

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Description

Secalciferol ((24R)-24,25-Dihydroxyvitamin D3) is the major active metabolite of Vitamin D. Secalciferol activates vitamin D receptor (VDR) with an EC50 value of 150 nM. Secalciferol is involved in a wide range of biological functions such as calcium homeostasis, cellular differentiation and proliferation processes, as well as other functions related to the immune system, which is promising for research of rickets, osteomalacia, hypercalcemia and autoimmune disorders[1][2][3][4][5].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Secalciferol of a part of the glucuronides is deconjugated by the enzyme from bacteria in the intestine and is probably reabsorbed and further oxidized to other metabolites in rat intestine[2].
Secalciferol is metabolized by β-glucuronidase to 24,25(OH)2D3-3G and -24G in rat bile[2].
Secalciferol (100 pM-10 μM, 24 h) dose-dependently activates vitamin D receptor (VDR) with an EC50 value of 150 nM, similar to that by Calcitriol (HY-10002) in the IZ-CYP24 and 12-VDRE cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Secalciferol Related Antibodies
In Vivo

Secalciferol (1-1000 μg/kg, i.p., daily for 28 days) dose dependently increases the length of the fifth lumbar spine in the hypophosphatemic (Hyp) mice[1].
Secalciferol (1 mg/kg, s.c., daily for 28 days) improves the rachitic bone and increases mineralized bone volume in the Hyp mice[1].
Secalciferol (0 or 5 ppm, powder fiet for 57 weeks) induces hyperplasia and neoplasia of the adrenal medulla with increase in cell prpliferative activity in rats[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Hyp mice[1]
Dosage: 1-1000 μg/kg
Administration: i.p., daily for 28 days
Result: Dose dependently increased the length of the fifth lumbar spine in the Hyp mice.
Animal Model: Hyp mice[1]
Dosage: 1 mg/kg
Administration: s.c., daily for 28 days and injected Calcein (8 mg/kg) (HY-D0040) on days 15 and 22
Result: Better improved the rachitic bone in the Hyp mice compared to vihecle.
Molecular Weight

416.64

Formula

C27H44O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C=C(CC1)/C(C[C@H]1O)=C\C=C2[C@@]3([H])[C@@](CCC\2)(C)[C@@H]([C@H](C)CC[C@@H](O)C(C)(C)O)CC3
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (240.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 50 mg/mL (120.01 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4002 mL 12.0008 mL 24.0015 mL
5 mM 0.4800 mL 2.4002 mL 4.8003 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.89%

COA (250 KB) HNMR (298 KB) RP-HPLC (266 KB) LCMS (87 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 2.4002 mL 12.0008 mL 24.0015 mL 60.0038 mL
5 mM 0.4800 mL 2.4002 mL 4.8003 mL 12.0008 mL
10 mM 0.2400 mL 1.2001 mL 2.4002 mL 6.0004 mL
15 mM 0.1600 mL 0.8001 mL 1.6001 mL 4.0003 mL
20 mM 0.1200 mL 0.6000 mL 1.2001 mL 3.0002 mL
25 mM 0.0960 mL 0.4800 mL 0.9601 mL 2.4002 mL
30 mM 0.0800 mL 0.4000 mL 0.8001 mL 2.0001 mL
40 mM 0.0600 mL 0.3000 mL 0.6000 mL 1.5001 mL
50 mM 0.0480 mL 0.2400 mL 0.4800 mL 1.2001 mL
60 mM 0.0400 mL 0.2000 mL 0.4000 mL 1.0001 mL
80 mM 0.0300 mL 0.1500 mL 0.3000 mL 0.7500 mL
100 mM 0.0240 mL 0.1200 mL 0.2400 mL 0.6000 mL
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Secalciferol Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Secalciferol55721-11-4(24R)-24,25-Dihydroxyvitamin D3Endogenous MetaboliteVD/VDRVitamin DVitamin D receptormetabolitecalcium homeostasiscellular differentiationproliferation processimmune systemricketsosteomalaciahypercalcemia and autoimmune disordersInhibitorinhibitorinhibit

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