2. GPCR/G Protein Neuronal Signaling Metabolic Enzyme/Protease
  3. 5-HT Receptor COMT Endogenous Metabolite
  4. Serotonin

Serotonin  (Synonyms: 5-Hydroxytryptamine; 5-HT)

Cat. No.: HY-B1473A Purity: 98.89%
COA Handling Instructions

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Serotonin is a monoamine neurotransmitter in the CNS and an endogenous 5-HT receptor agonist. Serotonin is also a catechol O-methyltransferase (COMT) inhibitor with a Ki of 44 μM.

For research use only. We do not sell to patients.

Serotonin Chemical Structure

Serotonin Chemical Structure

CAS No. : 50-67-9

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Based on 7 publication(s) in Google Scholar

Other Forms of Serotonin:

Serotonin Related Antibodies

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  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Serotonin is a monoamine neurotransmitter in the CNS and an endogenous 5-HT receptor agonist. Serotonin is also a catechol O-methyltransferase (COMT) inhibitor with a Ki of 44 μM.

IC50 & Target

Microbial Metabolite

 

Human Endogenous Metabolite

 

In Vitro

Serotonin is a monoamine neurotransmitter in the CNS and an endogenous 5-HT receptor agonist. Serotonin also inhibits catechol O-methyltransferase (COMT), an enzyme that contributes to modultion the perception of pain, via non-competitive binding to the site bound by catechol substrates with a binding affinity comparable to the binding affinity of catechol itself (Ki=?44 μM). Results show that addition of 100 μM of Serotonin decreases the reaction velocity of COMT[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Serotonin Related Antibodies
In Vivo

Serotonin produces robust hypersensitivity compare to saline-treated controls (p<0.001)[1]. A significant increase in colonic 5-HT content is observed in IL-13-/- mice receiving Serotonin compare to IL-13-/- receiving vehicle following induction of DSS colitis[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

176.22

Formula

C10H12N2O
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC1=CC2=C(NC=C2CCN)C=C1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (567.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.6747 mL 28.3736 mL 56.7472 mL
5 mM 1.1349 mL 5.6747 mL 11.3494 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.86%

COA (247 KB) HNMR (246 KB) RP-HPLC (239 KB) LCMS (95 KB) Elemental Analysis Report (106 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[2]

For macrophage culture, peritoneal cavity cells are collected from WT and IL-13-/- mice with or without dextran sodium sulfate (DSS) treatment and cultured. Cells are plated at a concentration of 3.0×106 cells per millilitre, and treated with either lipopolysaccharides (LPS); (100 ng/mL) or Serotonin (10-10 M) for 24 hours. The culture supernatant are collected and stored in -80°C until determination of cytokine levels using protein array system[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[2]

Dextran sodium sulfate (DSS) colitis is induced by orally administering 5% DSS in drinking water for 5 days. In a separate experiment, IL-13-/- mice are injected subcutaneously with 100 mg/kg of Serotonin (5-HTP) twice daily for 8 days beginning 3 days prior to induction of DSS colitis; whereas, the control IL-13-/- mice receive saline as vehicle. Animals are anaesthetized prior to euthanization via cervical dislocation at the conclusion of each experiment or if they reach a predetermined end point (ie, loss of ≥20% body weight and/or significant deterioration of body condition)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.6747 mL 28.3736 mL 56.7472 mL 141.8681 mL
5 mM 1.1349 mL 5.6747 mL 11.3494 mL 28.3736 mL
10 mM 0.5675 mL 2.8374 mL 5.6747 mL 14.1868 mL
15 mM 0.3783 mL 1.8916 mL 3.7831 mL 9.4579 mL
20 mM 0.2837 mL 1.4187 mL 2.8374 mL 7.0934 mL
25 mM 0.2270 mL 1.1349 mL 2.2699 mL 5.6747 mL
30 mM 0.1892 mL 0.9458 mL 1.8916 mL 4.7289 mL
40 mM 0.1419 mL 0.7093 mL 1.4187 mL 3.5467 mL
50 mM 0.1135 mL 0.5675 mL 1.1349 mL 2.8374 mL
60 mM 0.0946 mL 0.4729 mL 0.9458 mL 2.3645 mL
80 mM 0.0709 mL 0.3547 mL 0.7093 mL 1.7734 mL
100 mM 0.0567 mL 0.2837 mL 0.5675 mL 1.4187 mL
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Serotonin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Serotonin50-67-95-Hydroxytryptamine 5-HT5-HT ReceptorCOMTEndogenous MetaboliteSerotonin Receptor5-hydroxytryptamine ReceptorCatechol-O-methyltransferaseInhibitorinhibitorinhibit

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