Serotonin (Synonyms: 5-Hydroxytryptamine; 5-HT)

Name: Serotonin
Brand: MedChemExpress
SKU: HY-B1473A
Rating: 5.0 (8 reviews)

Cat. No.: HY-B1473A Purity: 98.89%: FAQs
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Serotonin is a monoamine neurotransmitter in the CNS and an endogenous 5-HT receptor agonist. Serotonin is also a catechol O-methyltransferase (COMT) inhibitor with a K_i of 44 μM.

For research use only. We do not sell to patients.

Serotonin Chemical Structure

CAS No. : 50-67-9

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1 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 96	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 195	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 320	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 520	In-stock	Estimated Time of Arrival: December 31
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Based on 8 publication(s) in Google Scholar

Other Forms of Serotonin:

Serotonin hydrochloride In-stock

8 Publications Citing Use of MCE Serotonin

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5-HT₁ Receptor 5-HT₂ Receptor 5-HT₃ Receptor 5-HT₄ Receptor 5-HT₅ Receptor 5-HT₆ Receptor 5-HT₇ Receptor 5-HT Receptor

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Serotonin is a monoamine neurotransmitter in the CNS and an endogenous 5-HT receptor agonist. Serotonin is also a catechol O-methyltransferase (COMT) inhibitor with a K_i of 44 μM.

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
B16	IC₅₀	> 30 μM Compound: 17	Antimelanogenic activity in mouse B16 cells assessed as inhibition of intracellular melanin accumulation after 2 days Antimelanogenic activity in mouse B16 cells assessed as inhibition of intracellular melanin accumulation after 2 days	[PMID: 19524439]
C6	EC₅₀	12 nM Compound: 5-HT	Agonist activity at human 5HT4 isoform E receptor expressed in rat C6 cells assessed as cAMP accumulation by radio-immunoassay relative to 5HT Agonist activity at human 5HT4 isoform E receptor expressed in rat C6 cells assessed as cAMP accumulation by radio-immunoassay relative to 5HT	[PMID: 19334715]
CHO	EC₅₀	0.7 nM Compound: 5-HT	Agonist activity assessed by stimulation of [35S]GTP-gamma-S binding to human 5HT1A receptor expressed in CHO cells Agonist activity assessed by stimulation of [35S]GTP-gamma-S binding to human 5HT1A receptor expressed in CHO cells	[PMID: 16458504]
CHO	EC₅₀	1 nM Compound: 5-HT	Agonistic activity at human 5HT1A in CHO cells by the inhibition of forskolin-stimulated cAMP accumulation Agonistic activity at human 5HT1A in CHO cells by the inhibition of forskolin-stimulated cAMP accumulation	[PMID: 16392798]
CHO	IC₅₀	1.2 x 10^-1 μM Compound: Serotonin	Displacement of [3H]LSD from human recombinant 5HT6 receptor expressed in CHO cells measured after 120 mins by scintillation counting method Displacement of [3H]LSD from human recombinant 5HT6 receptor expressed in CHO cells measured after 120 mins by scintillation counting method	[PMID: 27876250]
CHO	EC₅₀	1.5 nM Compound: 5-HT	Functional activity against human 5-hydroxytryptamine 2B receptor expressed in CHO cells using fluorometric imaging plate reader Functional activity against human 5-hydroxytryptamine 2B receptor expressed in CHO cells using fluorometric imaging plate reader	[PMID: 15081042]
CHO	EC₅₀	1.63 nM Compound: 5-HT	Agonist activity at 5HT2C receptor expressed in CHO cells by fluorescence based calcium mobilization assay Agonist activity at 5HT2C receptor expressed in CHO cells by fluorescence based calcium mobilization assay	[PMID: 22381048]
CHO	EC₅₀	1.7 nM Compound: 5-HT	Functional activity against human 5-hydroxytryptamine 2C receptor expressed in CHO cells using fluorometric imaging plate reader Functional activity against human 5-hydroxytryptamine 2C receptor expressed in CHO cells using fluorometric imaging plate reader	[PMID: 15081042]
CHO	EC₅₀	11 nM Compound: 5-HT	Functional activity against human 5-hydroxytryptamine 2A receptor expressed in CHO cells using fluorometric imaging plate reader Functional activity against human 5-hydroxytryptamine 2A receptor expressed in CHO cells using fluorometric imaging plate reader	[PMID: 15081042]
CHO	EC₅₀	150 nM Compound: 5-HT	Agonist activity at 5HT1A receptor expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at 5HT1A receptor expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 19559623]
CHO	IC₅₀	2.5 x 10^-10 M Compound: Serotonin	Displacement of [3H]LSD from human recombinant 5HT7 receptor expressed in CHO cells measured after 120 mins by scintillation counting method Displacement of [3H]LSD from human recombinant 5HT7 receptor expressed in CHO cells measured after 120 mins by scintillation counting method	[PMID: 27876250]
CHO	EC₅₀	25 nM Compound: 5-HT	Agonist activity at human recombinant 5HT2C receptor expressed in CHO K1 cells assessed as induction of calcium mobilization by FLIPR assay Agonist activity at human recombinant 5HT2C receptor expressed in CHO K1 cells assessed as induction of calcium mobilization by FLIPR assay	[PMID: 19646865]
CHO	EC₅₀	4.67 nM Compound: 1	The intrinsic activity was evaluated for its ability to inhibit forskolin-stimulated c-AMP formation mediated by cloned 5-hydroxytryptamine 1B receptor in CHO cell line; Full Agonist The intrinsic activity was evaluated for its ability to inhibit forskolin-stimulated c-AMP formation mediated by cloned 5-hydroxytryptamine 1B receptor in CHO cell line; Full Agonist	[PMID: 9871775]
CHO-K1	EC₅₀	0.07 μM Compound: Serotonin	Agonist activity at human recombinant 5-HT7 receptor expressed in CHOK1 Hunter cell incubated for 30 to 60 mins by hit-hunter cAMP assay based chemiluminescence analysis Agonist activity at human recombinant 5-HT7 receptor expressed in CHOK1 Hunter cell incubated for 30 to 60 mins by hit-hunter cAMP assay based chemiluminescence analysis	[PMID: 32631561]
CHO-K1	EC₅₀	15 nM Compound: Serotonin	Agonist activity at human recombinant 5HT7 receptor expressed in CHOK1 cells assessed as increase in cAMP levels by HTRF assay Agonist activity at human recombinant 5HT7 receptor expressed in CHOK1 cells assessed as increase in cAMP levels by HTRF assay	[PMID: 19326916]
CHO-K1	EC₅₀	15.33 nM Compound: 5-HT	Agonist activity at 5-HT1A receptor (unknown origin) expressed in CHOK1 cells co-expressing G-alpha 15 by Fluo-4 dye based FLIPR assay Agonist activity at 5-HT1A receptor (unknown origin) expressed in CHOK1 cells co-expressing G-alpha 15 by Fluo-4 dye based FLIPR assay	[PMID: 27487565]
CHO-K1	EC₅₀	16 nM Compound: 5-HT	Agonist activity at 5HT2A receptor (unknown origin) expressed in CHOK1 cells coexpressing Galpha15 by FLIPR assay Agonist activity at 5HT2A receptor (unknown origin) expressed in CHOK1 cells coexpressing Galpha15 by FLIPR assay	[PMID: 23675993]
CHO-K1	EC₅₀	18 nM Compound: Serotonin	Agonist activity at human recombinant 5HT1A receptor (unknown origin) stably expressed in CHO-K1 incubated for 30 mins by [35S]GTPgammaS binding assay Agonist activity at human recombinant 5HT1A receptor (unknown origin) stably expressed in CHO-K1 incubated for 30 mins by [35S]GTPgammaS binding assay	[PMID: 31627993]
CHO-K1	EC₅₀	39 nM Compound: 5-HT	Agonist activity at 5HT1A receptor (unknown origin) expressed in CHOK1 cells coexpressing Galpha15 by FLIPR assay Agonist activity at 5HT1A receptor (unknown origin) expressed in CHOK1 cells coexpressing Galpha15 by FLIPR assay	[PMID: 23675993]
CHO-K1	EC₅₀	4.82 nM Compound: 5-HT	Inhibition of forskolin-stimulated c-AMP formation by human 5-hydroxytryptamine 1B receptor expressed in CHO-K1 cells Inhibition of forskolin-stimulated c-AMP formation by human 5-hydroxytryptamine 1B receptor expressed in CHO-K1 cells	[PMID: 8960551]
CHO-K1	EC₅₀	45.3 nM Compound: Serotonin	Partial agonist activity at recombinant human 5-HT1A expressed in CHO-K1 cells by pathhunter beta-arrestin assay Partial agonist activity at recombinant human 5-HT1A expressed in CHO-K1 cells by pathhunter beta-arrestin assay	[PMID: 32631554]
CHO-K1	EC₅₀	47 nM Compound: 5-HT	Agonist activity at 5-HT2A receptor (unknown origin) expressed in CHOK1 cells co-expressing G-alpha 15 by Fluo-4 dye based FLIPR assay Agonist activity at 5-HT2A receptor (unknown origin) expressed in CHOK1 cells co-expressing G-alpha 15 by Fluo-4 dye based FLIPR assay	[PMID: 27487565]
Flp-In-293	EC₅₀	0.19 nM Compound: Serotonin	Agonist activity at recombinant human unedited 5HT2C receptor isoform INI expressed in Flp-In-293 cells assessed as increase in calcium influx measured for 300 secs by Fluo-4 dye based FLIPR assay Agonist activity at recombinant human unedited 5HT2C receptor isoform INI expressed in Flp-In-293 cells assessed as increase in calcium influx measured for 300 secs by Fluo-4 dye based FLIPR assay	[PMID: 31445232]
Flp-In-293	EC₅₀	0.27 nM Compound: Serotonin	Agonist activity at recombinant human 5HT2A receptor expressed in Flp-In-293 cells assessed as increase in calcium influx measured for 300 secs by Fluo-4 dye based FLIPR assay Agonist activity at recombinant human 5HT2A receptor expressed in Flp-In-293 cells assessed as increase in calcium influx measured for 300 secs by Fluo-4 dye based FLIPR assay	[PMID: 31445232]
Flp-In-293	EC₅₀	0.86 nM Compound: Serotonin	Agonist activity at recombinant human 5HT2B receptor expressed in Flp-In-293 cells assessed as increase in calcium influx measured for 300 secs by Fluo-4 dye based FLIPR assay Agonist activity at recombinant human 5HT2B receptor expressed in Flp-In-293 cells assessed as increase in calcium influx measured for 300 secs by Fluo-4 dye based FLIPR assay	[PMID: 31445232]
HEK293	EC₅₀	0.00087 μM Compound: 5-HT, serotonin	Agonist activity at human 5HT2C receptor expressed in HEK293 cells assessed as induction of intracellular calcium release measured for 90 secs by fluorescence assay Agonist activity at human 5HT2C receptor expressed in HEK293 cells assessed as induction of intracellular calcium release measured for 90 secs by fluorescence assay	[PMID: 23301527]
HEK293	EC₅₀	0.0026 μM Compound: 5-HT, serotonin	Agonist activity at human 5HT2A receptor expressed in HEK293 cells assessed as induction of intracellular calcium release measured for 90 secs by fluorescence assay Agonist activity at human 5HT2A receptor expressed in HEK293 cells assessed as induction of intracellular calcium release measured for 90 secs by fluorescence assay	[PMID: 23301527]
HEK293	EC₅₀	0.017 μM Compound: 5-HT, serotonin	Agonist activity at human 5-HT7 expressed in HEK-293 cells assessed as increase in cAMP level after 45 mins by HTRF assay Agonist activity at human 5-HT7 expressed in HEK-293 cells assessed as increase in cAMP level after 45 mins by HTRF assay	[PMID: 25073094]
HEK293	EC₅₀	0.044 μM Compound: 5-HT, serotonin	Agonist activity at human 5HT2C receptor expressed in HEK293 cells assessed as accumulation of IP1 incubated for 1 hr at 37 degC followed by 15 mins at RT by TR-FRET assay Agonist activity at human 5HT2C receptor expressed in HEK293 cells assessed as accumulation of IP1 incubated for 1 hr at 37 degC followed by 15 mins at RT by TR-FRET assay	[PMID: 23301527]
HEK293	EC₅₀	0.09 nM Compound: 5-HT	Agonist activity at human 5HT2C receptor expressed in HEK293 cells assessed as calcium flux measured every sec for 3 mins by FLIPR assay Agonist activity at human 5HT2C receptor expressed in HEK293 cells assessed as calcium flux measured every sec for 3 mins by FLIPR assay	[PMID: 22778800]
HEK293	EC₅₀	0.09 nM Compound: 1	Agonist activity at human 5HT2C receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay Agonist activity at human 5HT2C receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay	[PMID: 19284718]
HEK293	EC₅₀	0.1 μM Compound: serotonin	Activity at SER4 receptor expressed in HEK293 cells assessed as increase in calcium by calcium imaging assay Activity at SER4 receptor expressed in HEK293 cells assessed as increase in calcium by calcium imaging assay	[PMID: 18033297]
HEK293	EC₅₀	0.15 μM Compound: 5-HT, serotonin	Agonist activity at human 5HT2A receptor expressed in HEK293 cells assessed as accumulation of IP1 incubated for 1 hr at 37 degC followed by 15 mins at RT by TR-FRET assay Agonist activity at human 5HT2A receptor expressed in HEK293 cells assessed as accumulation of IP1 incubated for 1 hr at 37 degC followed by 15 mins at RT by TR-FRET assay	[PMID: 23301527]
HEK293	EC₅₀	0.17 nM Compound: 5-HT	Agonist activity at human 5-HT2C-INI receptor isoform expressed in Flp-IN HEK293 cells assessed as induction of Gq-mediated calcium flux measured every second for 5 mins by Fluo-4 dye based FLIPR assay Agonist activity at human 5-HT2C-INI receptor isoform expressed in Flp-IN HEK293 cells assessed as induction of Gq-mediated calcium flux measured every second for 5 mins by Fluo-4 dye based FLIPR assay	[PMID: 28657744]
HEK293	EC₅₀	0.21 nM Compound: 5-HT	Agonist activity at human 5-HT2C receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay Agonist activity at human 5-HT2C receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay	[PMID: 25633969]
HEK293	EC₅₀	0.4 nM Compound: 5-HT	Agonist activity at human 5-HT2C receptor expressed in HEK293 cells assessed as increase in calcium mobilization by FLIPR assay Agonist activity at human 5-HT2C receptor expressed in HEK293 cells assessed as increase in calcium mobilization by FLIPR assay	[PMID: 23602445]
HEK293	EC₅₀	0.41 nM Compound: 5-HT	Agonist activity at human 5HT2C receptor stably expressed in HEK293 cells co-expressing Gq assessed as induction of calcium mobilization measured at 1 sec interval for 60 secs by Fluo-4 calcium dye based FLIPR assay Agonist activity at human 5HT2C receptor stably expressed in HEK293 cells co-expressing Gq assessed as induction of calcium mobilization measured at 1 sec interval for 60 secs by Fluo-4 calcium dye based FLIPR assay	[PMID: 32282200]
HEK293	EC₅₀	0.5 nM Compound: 1	Agonist activity at human 5HT2B receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay Agonist activity at human 5HT2B receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay	[PMID: 19284718]
HEK293	EC₅₀	0.5 μM Compound: 5-HT	Antagonist activity at human 5HT3A receptor expressed in HEK293 cells by FMP assay Antagonist activity at human 5HT3A receptor expressed in HEK293 cells by FMP assay	[PMID: 17722904]
HEK293	EC₅₀	0.86 nM Compound: 5-HT	Agonist activity at human 5HT2B receptor expressed in HEK293 cells assessed as calcium flux measured every sec for 3 mins by FLIPR assay Agonist activity at human 5HT2B receptor expressed in HEK293 cells assessed as calcium flux measured every sec for 3 mins by FLIPR assay	[PMID: 22778800]
HEK293	EC₅₀	0.92 nM Compound: 5-HT	Agonist activity at human 5-HT2B receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay Agonist activity at human 5-HT2B receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay	[PMID: 25633969]
HEK293	EC₅₀	1.1 nM Compound: 5-HT	Agonist activity at human 5-HT2B receptor expressed in HEK293 cells assessed as increase in calcium mobilization by FLIPR assay Agonist activity at human 5-HT2B receptor expressed in HEK293 cells assessed as increase in calcium mobilization by FLIPR assay	[PMID: 23602445]
HEK293	EC₅₀	1.46 nM Compound: 5-HT	Agonist activity at human 5-HT2B receptor expressed in Flp-IN HEK293 cells assessed as induction of calcium flux measured every second for 5 mins by Fluo-4 dye based FLIPR assay Agonist activity at human 5-HT2B receptor expressed in Flp-IN HEK293 cells assessed as induction of calcium flux measured every second for 5 mins by Fluo-4 dye based FLIPR assay	[PMID: 28657744]
HEK293	EC₅₀	1.5 nM Compound: 5-HT	Agonist activity at human 5-HT2A receptor expressed in HEK293 cells assessed as increase in calcium mobilization by FLIPR assay Agonist activity at human 5-HT2A receptor expressed in HEK293 cells assessed as increase in calcium mobilization by FLIPR assay	[PMID: 23602445]
HEK293	IC₅₀	1.8 x 10^-1 μM Compound: Serotonin	Displacement of [3H]LSD from human recombinant 5HT5A receptor expressed in HEK293 cells measured after 120 mins by scintillation counting method Displacement of [3H]LSD from human recombinant 5HT5A receptor expressed in HEK293 cells measured after 120 mins by scintillation counting method	[PMID: 27876250]
HEK293	EC₅₀	1.88 nM Compound: 5-HT	Agonist activity at human 5-HT2A receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay Agonist activity at human 5-HT2A receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay	[PMID: 25633969]
HEK293	EC₅₀	2.35 nM Compound: 5-HT	Agonist activity at human 5-HT2A receptor expressed in Flp-IN HEK293 cells assessed as induction of calcium flux measured every second for 5 mins by Fluo-4 dye based FLIPR assay Agonist activity at human 5-HT2A receptor expressed in Flp-IN HEK293 cells assessed as induction of calcium flux measured every second for 5 mins by Fluo-4 dye based FLIPR assay	[PMID: 28657744]
HEK293	IC₅₀	260 nM Compound: Serotonin	Displacement of [3H]LSD from human recombinant 5-HT5a receptor in HEK293 cells after 120 mins by scintillation counting Displacement of [3H]LSD from human recombinant 5-HT5a receptor in HEK293 cells after 120 mins by scintillation counting	[PMID: 27997171]
HEK293	EC₅₀	3.75 nM Compound: 5-HT	Agonist activity at 5-HT1A receptor (unknown origin) expressed in HEK293 cells assessed as inhibition of [35S]GTPgammaS binding by scintillation proximity assay Agonist activity at 5-HT1A receptor (unknown origin) expressed in HEK293 cells assessed as inhibition of [35S]GTPgammaS binding by scintillation proximity assay	[PMID: 23332346]
HEK293	EC₅₀	5.8 nM Compound: 1	Agonist activity at human 5HT2A receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay Agonist activity at human 5HT2A receptor expressed in HEK293 cells assessed as calcium flux by FLIPR assay	[PMID: 19284718]
HEK293	EC₅₀	5.8 nM Compound: 5-HT	Agonist activity at human 5-HT7R expressed in HEK293 cells assessed as induction of cAMP levels treated for 10 mins measured after 30 mins by D2-dye based fluorescence assay Agonist activity at human 5-HT7R expressed in HEK293 cells assessed as induction of cAMP levels treated for 10 mins measured after 30 mins by D2-dye based fluorescence assay	[PMID: 27475109]
HEK293	EC₅₀	7.6 nM Compound: 5-HT	Agonist activity at human 5HT2A receptor expressed in HEK293 cells assessed as calcium flux measured every sec for 3 mins by FLIPR assay Agonist activity at human 5HT2A receptor expressed in HEK293 cells assessed as calcium flux measured every sec for 3 mins by FLIPR assay	[PMID: 22778800]
U2OS	IC₅₀	1.25 nM Compound: Serotonin	Antagonist activity at recombinant human 5-HT2C expressed in human U2OS cells by pathhunter beta-arrestin assay Antagonist activity at recombinant human 5-HT2C expressed in human U2OS cells by pathhunter beta-arrestin assay	[PMID: 32631554]
U2OS	IC₅₀	27.9 nM Compound: Serotonin	Antagonist activity at recombinant human 5-HT2A expressed in human U2OS cells by pathhunter beta-arrestin assay Antagonist activity at recombinant human 5-HT2A expressed in human U2OS cells by pathhunter beta-arrestin assay	[PMID: 32631554]

In Vitro

Serotonin is a monoamine neurotransmitter in the CNS and an endogenous 5-HT receptor agonist. Serotonin also inhibits catechol O-methyltransferase (COMT), an enzyme that contributes to modultion the perception of pain, via non-competitive binding to the site bound by catechol substrates with a binding affinity comparable to the binding affinity of catechol itself (K_i=?44 μM). Results show that addition of 100 μM of Serotonin decreases the reaction velocity of COMT^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Serotonin produces robust hypersensitivity compare to saline-treated controls (p<0.001)^[1]. A significant increase in colonic 5-HT content is observed in IL-13^-/- mice receiving Serotonin compare to IL-13^-/- receiving vehicle following induction of DSS colitis^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

176.22

Formula

C₁₀H₁₂N₂O

CAS No.

50-67-9

Appearance

Solid

Color

White to off-white

SMILES

OC1=CC2=C(NC=C2CCN)C=C1

Structure Classification

Initial Source

Microorganisms

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (567.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.6747 mL	28.3736 mL	56.7472 mL
5 mM	1.1349 mL	5.6747 mL	11.3494 mL
10 mM	0.5675 mL	2.8374 mL	5.6747 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.86%

Select Batch:

Data Sheet (276 KB) SDS (419 KB)

COA (247 KB) HNMR (246 KB) RP-HPLC (239 KB) LCMS (95 KB) Elemental Analysis Report (106 KB)

Handling Instructions (2659 KB)

References

[1]. Tsao D, et al. Serotonin-induced hypersensitivity via inhibition of catechol O-methyltransferase activity. Mol Pain. 2012 Apr 13;8:25. [Content Brief]

[2]. Shajib MS, et al. Interleukin 13 and serotonin: linking the immune and endocrine systems in murine models of intestinal inflammation. PLoS One. 2013 Aug 28;8(8):e72774. [Content Brief]

Cell Assay ^[2]	For macrophage culture, peritoneal cavity cells are collected from WT and IL-13^-/- mice with or without dextran sodium sulfate (DSS) treatment and cultured. Cells are plated at a concentration of 3.0×10⁶ cells per millilitre, and treated with either lipopolysaccharides (LPS); (100 ng/mL) or Serotonin (10^-10 M) for 24 hours. The culture supernatant are collected and stored in -80°C until determination of cytokine levels using protein array system^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[2]	Dextran sodium sulfate (DSS) colitis is induced by orally administering 5% DSS in drinking water for 5 days. In a separate experiment, IL-13^-/- mice are injected subcutaneously with 100 mg/kg of Serotonin (5-HTP) twice daily for 8 days beginning 3 days prior to induction of DSS colitis; whereas, the control IL-13^-/- mice receive saline as vehicle. Animals are anaesthetized prior to euthanization via cervical dislocation at the conclusion of each experiment or if they reach a predetermined end point (ie, loss of ≥20% body weight and/or significant deterioration of body condition)^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Tsao D, et al. Serotonin-induced hypersensitivity via inhibition of catechol O-methyltransferase activity. Mol Pain. 2012 Apr 13;8:25. [Content Brief] [2]. Shajib MS, et al. Interleukin 13 and serotonin: linking the immune and endocrine systems in murine models of intestinal inflammation. PLoS One. 2013 Aug 28;8(8):e72774. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.6747 mL	28.3736 mL	56.7472 mL	141.8681 mL
	5 mM	1.1349 mL	5.6747 mL	11.3494 mL	28.3736 mL
	10 mM	0.5675 mL	2.8374 mL	5.6747 mL	14.1868 mL
	15 mM	0.3783 mL	1.8916 mL	3.7831 mL	9.4579 mL
	20 mM	0.2837 mL	1.4187 mL	2.8374 mL	7.0934 mL
	25 mM	0.2270 mL	1.1349 mL	2.2699 mL	5.6747 mL
	30 mM	0.1892 mL	0.9458 mL	1.8916 mL	4.7289 mL
	40 mM	0.1419 mL	0.7093 mL	1.4187 mL	3.5467 mL
	50 mM	0.1135 mL	0.5675 mL	1.1349 mL	2.8374 mL
	60 mM	0.0946 mL	0.4729 mL	0.9458 mL	2.3645 mL
	80 mM	0.0709 mL	0.3547 mL	0.7093 mL	1.7734 mL
	100 mM	0.0567 mL	0.2837 mL	0.5675 mL	1.4187 mL

Serotonin Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Serotonin50-67-95-Hydroxytryptamine 5-HT5-HT ReceptorCOMTEndogenous MetaboliteSerotonin Receptor5-hydroxytryptamine ReceptorCatechol-O-methyltransferaseInhibitorinhibitorinhibit

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Serotonin (Synonyms: 5-Hydroxytryptamine; 5-HT)

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Complete Stock Solution Preparation Table

Serotonin Related Classifications

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