2. Anti-infection
  3. Bacterial Antibiotic
  4. Spectinomycin dihydrochloride pentahydrate

Spectinomycin dihydrochloride pentahydrate  (Synonyms: Spectinomycin hydrochloride hydrate)

Cat. No.: HY-B1828A Purity: ≥98.0%
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Spectinomycin dihydrochloride pentahydrate is a broad-spectrum antibiotic and inhibits the growth of a variety of gram-positive and gram-negative organisms. Spectinomycin dihydrochloride pentahydrate acts by selectively targeting to the bacterial ribosome and interrupting protein synthesis. Spectinomycin dihydrochloride pentahydrate is also a noncompetitive inhibitor of td intron RNA with an Ki value of 7.2 mM-.

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Spectinomycin dihydrochloride pentahydrate Chemical Structure

Spectinomycin dihydrochloride pentahydrate Chemical Structure

CAS No. : 22189-32-8

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Based on 3 publication(s) in Google Scholar

Other Forms of Spectinomycin dihydrochloride pentahydrate:

Spectinomycin dihydrochloride pentahydrate Related Antibodies

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Description

Spectinomycin dihydrochloride pentahydrate is a broad-spectrum antibiotic and inhibits the growth of a variety of gram-positive and gram-negative organisms. Spectinomycin dihydrochloride pentahydrate acts by selectively targeting to the bacterial ribosome and interrupting protein synthesis. Spectinomycin dihydrochloride pentahydrate is also a noncompetitive inhibitor of td intron RNA with an Ki value of 7.2 mM[1]-[5].

IC50 & Target

bacterial ribosomal subunit[5]
In Vitro

Spectinomycin dihydrochloride pentahydrate selectively inhibits protein synthesis in cells and in extracts of Escherichia coli:
Spectinomycin dihydrochloride pentahydrate (50 μg/mL) inhibits Escherichia coli growth rapidly and reversibly, and suppresses amino acid incorporation immediately[1].
Spectinomycin dihydrochloride pentahydrate (1 μg/mL or 3 μM) inhibits polypeptide synthesis directed either by endogenous messenger RNA or by MS-2 bacteriophage RNA, with maximum inhibition of 70-80% in extracts of Escherichia coli[1].
Spectinomycin dihydrochloride pentahydrate blocks the translocation of peptidyl-tRNAs from A-site to P-site by inhibiting the binding of elongation factor G to the ribosome[2].
Spectinomycin dihydrochloride pentahydrate interacts specifically with the residues G1064 and 01192 in 16S rRNA and potentially makes it inactive[2].
Spectinomycin dihydrochloride pentahydrate exhibits splicing inhibition and dependent on pH changes and Mg2+ concentration, indicating electrostatic interactions with the intron RNA[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Spectinomycin dihydrochloride pentahydrate Related Antibodies
In Vivo

Spectinomycin dihydrochloride pentahydrate (20 mg/kg; i.m.; 20-100 mg/kg; 9 d) shows the safety in healthy chicks[4].
Spectinomycin dihydrochloride pentahydrate (10 mg/kg; i.v.; single dose) has the major elimination pathway by renal excretion, approximately 55% is excreted into the urine in unchanged form[5].
Pharmacokinetics of Spectinomycin dihydrochloride pentahydrate in Rat[5]

Parameter C0 (μg/mL) AUC0-∞ (μg?h/mL) Vd (L/kg) CL (L/h/kg) MRT (h) T1/2α (h) T1/2β (h) T1/2γ (h) fe CLrenal (L/h/kg) Eratio
Non atrioventricular analysis 44.3 16.8 0.756 0.602 0.757 / / / 0.553 0.359 1.00
Three-compartment model 37.8 15.7 0.747 0.649 1.11 / 0.237 0.754 19.5 / /

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Arbor Acres plus broiler chicks (15-day-old)[4]
Dosage: 20 mg/kg, 60 mg/kg, 100 mg/kg
Administration: Intramuscular injection (chest muscles); 9 days
Result: Showed biosecurity of 20 mg/kg by complete blood count, biochemical parameters, histopathological, clinical signs, body weight gain, and feed conversion ratio (FCR).
Resulted minor toxicity of 60 mg/kg.
Molecular Weight

495.35

Formula

C14H36Cl2N2O12
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1C[C@@H](C)O[C@@]2([H])O[C@@]3([H])[C@]([C@@H](NC)[C@@H](O)[C@@H](NC)[C@@H]3O)([H])O[C@]21O.[H]Cl.[H]Cl.[5H2O]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 62.5 mg/mL (126.17 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0188 mL 10.0939 mL 20.1877 mL
5 mM 0.4038 mL 2.0188 mL 4.0375 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (100.94 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

SDS (393 KB)

COA (260 KB) HNMR (199 KB) MS (163 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.0188 mL 10.0939 mL 20.1877 mL 50.4694 mL
5 mM 0.4038 mL 2.0188 mL 4.0375 mL 10.0939 mL
10 mM 0.2019 mL 1.0094 mL 2.0188 mL 5.0469 mL
15 mM 0.1346 mL 0.6729 mL 1.3458 mL 3.3646 mL
20 mM 0.1009 mL 0.5047 mL 1.0094 mL 2.5235 mL
25 mM 0.0808 mL 0.4038 mL 0.8075 mL 2.0188 mL
30 mM 0.0673 mL 0.3365 mL 0.6729 mL 1.6823 mL
40 mM 0.0505 mL 0.2523 mL 0.5047 mL 1.2617 mL
50 mM 0.0404 mL 0.2019 mL 0.4038 mL 1.0094 mL
60 mM 0.0336 mL 0.1682 mL 0.3365 mL 0.8412 mL
80 mM 0.0252 mL 0.1262 mL 0.2523 mL 0.6309 mL
100 mM 0.0202 mL 0.1009 mL 0.2019 mL 0.5047 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Spectinomycin dihydrochloride pentahydrate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Spectinomycin dihydrochloride22189-32-8Spectinomycin hydrochlorideBacterialAntibioticbiosecurityaminocyclitolbroad-spectrumgram-positivegram-negativeorganismbacterial ribosomeEscherichia coliamino acid incorporationMS-2 bacteriophagepeptidyl-tRNAelectrostatic interactionInhibitorinhibitorinhibit

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