Separase

Separin; Esp1; EspL1

Separase is a cysteine protease and endopeptidase belonging to the CD clan protease family. Separase can promote sister chromatid separation by cleaving the Scc1/Rad21 subunit of cohesin. Separase can also cleave Mcd1. Its activity is tightly regulated, and it has complex inhibitory interactions with securin, cyclin B1/CDK1, etc., and is also regulated by PP2A, etc. Separase is closely related to a variety of cancers. In breast cancer, its abnormal expression can predict the prognosis of patients. High mitotic Separase indicates a poor prognosis, while diffuse Separase is associated with a survival advantage. In ovarian cancer, ESPL1 (encoding Separase) is differentially expressed in high-grade serous ovarian cancer, and the expression level is higher than that in normal ovarian tissue. In mouse models, overexpression of Separase can induce aneuploidy and tumorigenesis of mammary epithelial cells. In addition, Separase is also involved in the apoptosis process of yeast cells^[1]^[2]^[3]^[4]^[5]^[6]^[7].

References:

[1]. Gurvits N, et al. Separase is a marker for prognosis and mitotic activity in breast cancer. Br J Cancer. 2017 Oct 24;117(9):1383-1391. [Content Brief]

[2]. Yu J, et al. The molecular mechanisms of human separase regulation. Biochem Soc Trans. 2023 Jun 28;51(3):1225-1233. [Content Brief]

[3]. Wirth KG, et al. Separase: a universal trigger for sister chromatid disjunction but not chromosome cycle progression. J Cell Biol. 2006 Mar 13;172(6):847-60. [Content Brief]

[4]. Pemberton HN, et al. Separase, securin and Rad21 in neural cell growth. J Cell Physiol. 2007 Oct;213(1):45-53. [Content Brief]

[5]. Zhang N, et al. Overexpression of Separase induces aneuploidy and mammary tumorigenesis. Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):13033-8. [Content Brief]

[6]. Yang H, et al. Cleavage of Mcd1 by caspase-like protease Esp1 promotes apoptosis in budding yeast. Mol Biol Cell. 2008 May;19(5):2127-34. [Content Brief]

[7]. Mamoor S. ESPL1 (Separase) is differentially expressed in high-grade serous ovarian cancers.

Separase Inhibitors (2)

Separase Related Products (2):

Cat. No.	Product Name	Effect	Purity	Chemical Structure

HY-117522

Sepin-1	Inhibitor
Sepin-1 is a potent separase inhibitor with an IC₅₀ value of 14.8 µM. Sepin-1 inhibits cell proliferation, migration and wound healing. Sepin-1 decreases the expression of FoxM1 protein and mRNA level. Sepin-1 shows anti-tumor activity.

HY-138084

SIC5-6	Inhibitor	99.82%
SIC5-6 is a potent Separase inhibitor. Separase, a large cysteine protease, involves in chromosome segregation during mitosis and meiosis, DNA damage repair, centrosome disengagement and duplication, spindle stabilization and elongation. Separase is highly overexpressed in many solid cancers, serves as an attractive chemotherapeutic target.

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