3-Methyl-L-histidine

Name: 3-Methyl-L-histidine
Brand: MedChemExpress
SKU: HY-W017007
Rating: 4.5 (1 reviews)

Cat. No.: HY-W017007 Purity: 99.69%: Data Sheet
SDS

COA
Handling Instructions Technical Support

Solubility

In Vivo Dissolution Calculator

3-Methyl-L-histidine is an endogenous amino acid that can be found in actin and myosin.

For research use only. We do not sell to patients.

3-Methyl-L-histidine Chemical Structure

CAS No. : 368-16-1

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	USD 99	In-stock	Estimated Time of Arrival: April 2
Solution
10 mM * 1 mL in Water	USD 104	In-stock	Estimated Time of Arrival: April 2
Solid
100 mg	USD 95	In-stock	Estimated Time of Arrival: April 2
200 mg		Get quote
500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of 3-Methyl-L-histidine:

3-Methyl-L-histidine-d₃ Get quote
3-Methyl-L-histidine-d₃ hydrochloride Get quote
3-Methyl-D-histidine-d₃ hydrochloride Get quote

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•Related Screening Libraries:

•Related Small Molecules:

Biological Activity
Purity & Documentation
References
Customer Review

Description

3-Methyl-L-histidine is an endogenous amino acid that can be found in actin and myosin^[1].

In Vitro

3-Methyl-L-histidine could be used as a robust, unequivocal index of that proportion of the total protein in food products derived from lean meat. 3-Methyl-L-histidine is absent from soya protein^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Adherence to high chicken protein diet (HCD) alleviated excessive weight gain and glycemic control regardless of the presence of gut microbiota in apolipoprotein E-deficient mice. In contrast, long-term HCD administration enhanced intestinal cholesterol absorption and accelerated atherosclerotic plaque formation in a gut microbiota-dependent manner. 3-Methyl-L-histidine results from an enrichment of Lachnospiraceae, as the key microbial effector to the atherogenic effect of HCD. 3-Methyl-L-histidine facilitates the binding of hepatocyte nuclear factor 1A (HNF1A) to the promoter of NPC1-like intracellular cholesterol transporter 1 (NPC1L1)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

169.18

Formula

C₇H₁₁N₃O₂

CAS No.

368-16-1

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)[C@@H](N)CC1=CN=CN1C

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (591.07 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.9107 mL	29.5536 mL	59.1072 mL
5 mM	1.1821 mL	5.9107 mL	11.8214 mL
10 mM	0.5911 mL	2.9554 mL	5.9107 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 50 mg/mL (295.54 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Purity & Documentation

Purity: 99.85%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (249 KB)HNMR (174 KB)LCMS (396 KB)

Handling Instructions (2659 KB)

References

[1]. White WJ, Lawrie RA. Variations in the levels of 3-methyl-l-histidine of the myosins within the bovine carcass. Meat Sci. 1985;15(3):173-81. [Content Brief]

[2]. Zhu S, et al. Microbiota-derived 3-Methyl-L-histidine mediates the proatherogenic effect of high chicken protein diet. MedComm (2020). 2025 Feb 13;6(2):e70090. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	5.9107 mL	29.5536 mL	59.1072 mL	147.7681 mL
	5 mM	1.1821 mL	5.9107 mL	11.8214 mL	29.5536 mL
	10 mM	0.5911 mL	2.9554 mL	5.9107 mL	14.7768 mL
	15 mM	0.3940 mL	1.9702 mL	3.9405 mL	9.8512 mL
	20 mM	0.2955 mL	1.4777 mL	2.9554 mL	7.3884 mL
	25 mM	0.2364 mL	1.1821 mL	2.3643 mL	5.9107 mL
	30 mM	0.1970 mL	0.9851 mL	1.9702 mL	4.9256 mL
	40 mM	0.1478 mL	0.7388 mL	1.4777 mL	3.6942 mL
	50 mM	0.1182 mL	0.5911 mL	1.1821 mL	2.9554 mL
	60 mM	0.0985 mL	0.4926 mL	0.9851 mL	2.4628 mL
	80 mM	0.0739 mL	0.3694 mL	0.7388 mL	1.8471 mL
	100 mM	0.0591 mL	0.2955 mL	0.5911 mL	1.4777 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.