A 419259 (Synonyms: RK-20449)

Cat. No.: HY-15764G: FAQs
Data Sheet Handling Instructions Technical Support

A 419259 GMP is the GMP grade A 419259 (HY-15764), inducing cell apoptosis. GMP-grade small molecules can be used as auxiliary reagents in cell therapy. A 419259 (RK-20449) is a broad-spectrum pyrrole-pyrimidine inhibitor targeting Src, Lck, and Lyn with IC₅₀s of 9 nM, <3 nM, and <3 nM, respectively.

For research use only. We do not sell to patients.

A 419259 Chemical Structure

CAS No. : 364042-47-7

Size	Stock	Quantity
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Other Forms of A 419259:

A 419259 trihydrochloride In-stock

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: 9 nM (Src), <3 nM (Lck), <3 nM (Lyn), 3 μM (Abl)^[1]

In Vitro

A419259 is a second-generation pyrrolopyrimidine that blocks proliferation and induces apoptosis in CML cell lines. It induces apoptosis in K-562 cells and also inhibits Meg-01 proliferation (IC₅₀=0.1 μM)^[1].
In the absence of IL-3, A-419259 strongly inhibits DAGM/Bcr-Abl cell proliferation (IC₅₀=0.1-0.3 μM)^[1].
A-419259 also inhibits overall SFK activity in CML cell lines and blocks Src kinase activation (IC₅₀=0.1-0.3 μM)^[1].
A 419259 GMP (1 μM; 16 h) inhibits endogenous SFK (c-Src and Lck) activity, thereby inhibiting Src-driven differentiation of mES cells toward primitive ectoderm-like cells^[2].
A 419259 GMP (0.3, 1 μM; 5 days) has no effect on undifferentiated colony morphology of hES cells grown in mTeSR medium^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

482.63

Formula

C₂₉H₃₄N₆O

CAS No.

364042-47-7

Appearance

Solid

Color

White to light yellow

SMILES

NC1=C2C(N([C@H]3CC[C@H](N4CCN(C)CC4)CC3)C=C2C5=CC=C(OC6=CC=CC=C6)C=C5)=NC=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (207.20 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0720 mL	10.3599 mL	20.7198 mL
5 mM	0.4144 mL	2.0720 mL	4.1440 mL
10 mM	0.2072 mL	1.0360 mL	2.0720 mL

View the Complete Stock Solution Preparation Table

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Wilson MB, et al. Selective pyrrolo-pyrimidine inhibitors reveal a necessary role for Src family kinases in Bcr-Abl signal transduction and oncogenesis. Oncogene. 2002 Nov 21;21(53):8075-88. [Content Brief]

[2]. Zhang X, et al. Src-family tyrosine kinase activities are essential for differentiation of human embryonic stem cells. Stem Cell Res. 2014 Nov;13(3 Pt A):379-89. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0720 mL	10.3599 mL	20.7198 mL	51.7995 mL
	5 mM	0.4144 mL	2.0720 mL	4.1440 mL	10.3599 mL
	10 mM	0.2072 mL	1.0360 mL	2.0720 mL	5.1800 mL
	15 mM	0.1381 mL	0.6907 mL	1.3813 mL	3.4533 mL
	20 mM	0.1036 mL	0.5180 mL	1.0360 mL	2.5900 mL
	25 mM	0.0829 mL	0.4144 mL	0.8288 mL	2.0720 mL
	30 mM	0.0691 mL	0.3453 mL	0.6907 mL	1.7267 mL
	40 mM	0.0518 mL	0.2590 mL	0.5180 mL	1.2950 mL
	50 mM	0.0414 mL	0.2072 mL	0.4144 mL	1.0360 mL
	60 mM	0.0345 mL	0.1727 mL	0.3453 mL	0.8633 mL
	80 mM	0.0259 mL	0.1295 mL	0.2590 mL	0.6475 mL
	100 mM	0.0207 mL	0.1036 mL	0.2072 mL	0.5180 mL

A 419259 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

A 419259 (GMP)364042-47-7RK-20449 (GMP)SrcApoptosisInhibitorinhibitorinhibit

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