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  4. AC-green

AC-green (VDP-green) is a β-allyl carbamate fluorescent probe for specifically imaging vicinal dithiol proteins (VDPs) in living systems (λex/λem=400/475 nm). AC-green can detect the reduced bovine serum albumin (rBSA) with high sensitivity. AC-green displays low toxicity and features high sensitivity, and is suitable for sensing VDPs in living cells and zebrafishes.

For research use only. We do not sell to patients.

AC-green Chemical Structure

AC-green Chemical Structure

CAS No. : 2937705-58-1

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5 mg USD 490 In-stock
10 mg USD 830 In-stock
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Based on 1 publication(s) in Google Scholar

AC-green Related Antibodies

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Description

AC-green (VDP-green) is a β-allyl carbamate fluorescent probe for specifically imaging vicinal dithiol proteins (VDPs) in living systems (λex/λem=400/475 nm). AC-green can detect the reduced bovine serum albumin (rBSA) with high sensitivity. AC-green displays low toxicity and features high sensitivity, and is suitable for sensing VDPs in living cells and zebrafishes[1].

In Vitro

AC-green (VDP-green) can respond to VDPs with more than 60-fold increase of emission in aqueous solution, while there is no significant interference from biological thiols, amino acids or inorganic salts[1].
AC-green (2 μM; 90 min) has no apparent fluorescence signal within the pH range of 5.0-9.0. Addition of rBSA turns on the fluorescence[1].
AC-gree (10 μM) has low cytotoxicity in HepG2 cells and Hela cells[1].
AC-gree (10 μM; for 15 min) images VDPs in living HepG2 cells and bright green fluorescence appeared. This fluorescence is inhibited when the cells are pretreated with PAO, a popular specificligand for protein vicinal dithiols[1].
AC-gree (10 μM; for 20 min) incubates with zebrafishes has a strong fluorescence signal appeared in the green channel[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AC-green Related Antibodies
Molecular Weight

435.47

Formula

C24H25N3O5
CAS No.
Appearance

Solid

Color

Light yellow to yellow

Emission (Em)

475
Excitation (Ex)

400
SMILES

O=C(NC1=CC2=CC=C(C=C2OC1=O)N(CC)CC)C(COC(NC3=CC=CC=C3)=O)=C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (229.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2964 mL 11.4818 mL 22.9637 mL
5 mM 0.4593 mL 2.2964 mL 4.5927 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2964 mL 11.4818 mL 22.9637 mL 57.4092 mL
5 mM 0.4593 mL 2.2964 mL 4.5927 mL 11.4818 mL
10 mM 0.2296 mL 1.1482 mL 2.2964 mL 5.7409 mL
15 mM 0.1531 mL 0.7655 mL 1.5309 mL 3.8273 mL
20 mM 0.1148 mL 0.5741 mL 1.1482 mL 2.8705 mL
25 mM 0.0919 mL 0.4593 mL 0.9185 mL 2.2964 mL
30 mM 0.0765 mL 0.3827 mL 0.7655 mL 1.9136 mL
40 mM 0.0574 mL 0.2870 mL 0.5741 mL 1.4352 mL
50 mM 0.0459 mL 0.2296 mL 0.4593 mL 1.1482 mL
60 mM 0.0383 mL 0.1914 mL 0.3827 mL 0.9568 mL
80 mM 0.0287 mL 0.1435 mL 0.2870 mL 0.7176 mL
100 mM 0.0230 mL 0.1148 mL 0.2296 mL 0.5741 mL
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AC-green Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AC-green2937705-58-1VDP-greenFluorescent Dyeβ-allyl carbamatevicinaldithiolproteinsVDPsbovineserumalbuminrBSAHepG2HelaInhibitorinhibitorinhibit

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