2. Apoptosis
  3. Caspase
  4. Ac-LEHD-AMC

Ac-LEHD-AMC is a fluorogenic substrate for caspase-9 (Excitation: 341 nm; Emission: 441 nm). Caspase-9 can induce hydrolysis of Ac-LEHD-AMC, resulting in the release of AMC fluorophore and its fluorescence can be used to quantify caspase-9 activity.

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Ac-LEHD-AMC Chemical Structure

Ac-LEHD-AMC Chemical Structure

CAS No. : 292633-16-0

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Based on 1 publication(s) in Google Scholar

Ac-LEHD-AMC Related Antibodies

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Description

Ac-LEHD-AMC is a fluorogenic substrate for caspase-9 (Excitation: 341 nm; Emission: 441 nm). Caspase-9 can induce hydrolysis of Ac-LEHD-AMC, resulting in the release of AMC fluorophore and its fluorescence can be used to quantify caspase-9 activity[1].

Molecular Weight

711.72

Formula

C33H41N7O11
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(C1=CC=C(NC([C@H](CC(O)=O)NC([C@@H](NC([C@H](CCC(O)=O)NC([C@@H](NC(C)=O)CC(C)C)=O)=O)CC2=CN=CN2)=O)=O)C=C1O3)=CC3=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (351.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4050 mL 7.0252 mL 14.0505 mL
5 mM 0.2810 mL 1.4050 mL 2.8101 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4050 mL 7.0252 mL 14.0505 mL 35.1262 mL
5 mM 0.2810 mL 1.4050 mL 2.8101 mL 7.0252 mL
10 mM 0.1405 mL 0.7025 mL 1.4050 mL 3.5126 mL
15 mM 0.0937 mL 0.4683 mL 0.9367 mL 2.3417 mL
20 mM 0.0703 mL 0.3513 mL 0.7025 mL 1.7563 mL
25 mM 0.0562 mL 0.2810 mL 0.5620 mL 1.4050 mL
30 mM 0.0468 mL 0.2342 mL 0.4683 mL 1.1709 mL
40 mM 0.0351 mL 0.1756 mL 0.3513 mL 0.8782 mL
50 mM 0.0281 mL 0.1405 mL 0.2810 mL 0.7025 mL
60 mM 0.0234 mL 0.1171 mL 0.2342 mL 0.5854 mL
80 mM 0.0176 mL 0.0878 mL 0.1756 mL 0.4391 mL
100 mM 0.0141 mL 0.0703 mL 0.1405 mL 0.3513 mL
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Ac-LEHD-AMC Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ac-LEHD-AMC292633-16-0CaspaseInhibitorinhibitorinhibit

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