Acesulfame potassium

Name: Acesulfame potassium
Brand: MedChemExpress
SKU: HY-D0195
Rating: 4.5 (1 reviews)

Cat. No.: HY-D0195 Purity: 99.38%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Acesulfame potassium is a synthetic sweetener. Long-term use of Acesulfame potassium can affect cognitive function, possibly by altering the neurometabolic functions in mice. Acesulfame potassium can suppress autophagic degradation of PD-L1 in RIL-175 and SK-Hep1 cells through the ERK1/2-mTORC1-ULK1 pathway, which may be related to immune evasion in cancer cells. Acesulfame potassium can be used in research on neurological diseases, metabolic disorders, cancer, and immune evasion.

For research use only. We do not sell to patients.

Acesulfame potassium Chemical Structure

CAS No. : 55589-62-3

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Acesulfame potassium:

Acesulfame-d₄ potassium Get quote
Acesulfame Get quote
Acesulfame (potassium) (Standard) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Acesulfame potassium (1 mM, 24 hours) upregulates PD-L1 protein levels in RIL-175 and SK-Hep1 cells^[2].
Acesulfame potassium (1 mM, 24 hours) increases granzyme B production in RIL-175 and SK-Hep1 cells co-cultured with T cells^[2].
Acesulfame potassium (1 mM, 24 hours) activates ERK1/2-mTORC1-ULK1 pathway to suppress autophagic degradation of PD-L1 in RIL-175 and SK-Hep1 cells^[2].
Acesulfame potassium (5-25 mM, 24 hours) inhibites mitochondrial metabolism in SH-SY5Y cells^[4].
Acesulfame potassium (5-25 mM, 24 hours) inhibites ATP production in cells and reduced phosphorylation of neuroprotective proteins, impaires energy metabolism and neuroprotective functions in SH-SY5Y cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:	RIL-175 (mouse hepatocellular carcinoma), SK-Hep1 (human hepatocellular carcinoma)
Concentration:	1 mM
Incubation Time:	24 hours
Result:	Significantly increased PD-L1 protein levels.

Cell Viability Assay^[4]

Cell Line:	SH-SY5Y (neuroblastoma cells)
Concentration:	5-25 mM
Incubation Time:	24 hours
Result:	Reduced the extracellular acidification rate (ECAR) in SH-SY5Y cells.

In Vivo

Acesulfame potassium (37.5 mg/kg, p.o., once daily for 4 weeks) increases body weight in male CD-1 mice and altered their gut microbiome composition^[3].
Long-term intake of Acesulfame potassium (12.5 mM, administered via drinking water for 40 weeks) affects the neurometabolic functions in C57BL/6J mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	CD-1 mice (male and female)^[3]
Dosage:	37.5 mg/kg
Administration:	Oral gavage (p.o.), once daily for 4 weeks
Result:	Increased body weight in male CD-1 mice. Significantly altered the gut microbiome composition in both male and female mice.

Animal Model:	C57BL/6J mice^[4]
Dosage:	12.5 mM
Administration:	Administered via drinking water, once daily for 40 weeks
Result:	Significant increase in fasting blood glucose and insulin levels in treated mice, without altering insulin sensitivity.

Molecular Weight

201.24

Formula

C₄H₄KNO₄S

CAS No.

55589-62-3

Appearance

Solid

Color

White to off-white

SMILES

O=C1[N-]S(OC(C)=C1)(=O)=O.[K+]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (496.92 mM; Need ultrasonic)

DMSO : 50 mg/mL (248.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.9692 mL	24.8460 mL	49.6919 mL
5 mM	0.9938 mL	4.9692 mL	9.9384 mL
10 mM	0.4969 mL	2.4846 mL	4.9692 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (12.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.38%

Select Batch:

Data Sheet (310 KB) SDS (393 KB)

COA (254 KB) HNMR (131 KB) RP-HPLC (206 KB) LCMS (100 KB)

Handling Instructions (2659 KB)

References

[1]. Cong WN, et al. Long-term artificial sweetener acesulfame potassium treatment alters neurometabolic functions in C57BL/6J mice. PLoS One. 2013 Aug 7;8(8):e70257. [Content Brief]

[2]. Kim DH, et al. Acesulfame potassium upregulates PD-L1 in HCC cells by attenuating autophagic degradation. Biochem Biophys Res Commun. 2024 Jun 4;711:149921. [Content Brief]

[3]. Bian X, et al. The artificial sweetener acesulfame potassium affects the gut microbiome and body weight gain in CD-1 mice. PLoS One. 2017 Jun 8;12(6):e0178426. [Content Brief]

[4]. Cong WN, et al. Long-term artificial sweetener acesulfame potassium treatment alters neurometabolic functions in C57BL/6J mice. PLoS One. 2013 Aug 7;8(8):e70257. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	4.9692 mL	24.8460 mL	49.6919 mL	124.2298 mL
	5 mM	0.9938 mL	4.9692 mL	9.9384 mL	24.8460 mL
	10 mM	0.4969 mL	2.4846 mL	4.9692 mL	12.4230 mL
	15 mM	0.3313 mL	1.6564 mL	3.3128 mL	8.2820 mL
	20 mM	0.2485 mL	1.2423 mL	2.4846 mL	6.2115 mL
	25 mM	0.1988 mL	0.9938 mL	1.9877 mL	4.9692 mL
	30 mM	0.1656 mL	0.8282 mL	1.6564 mL	4.1410 mL
	40 mM	0.1242 mL	0.6211 mL	1.2423 mL	3.1057 mL
	50 mM	0.0994 mL	0.4969 mL	0.9938 mL	2.4846 mL
	60 mM	0.0828 mL	0.4141 mL	0.8282 mL	2.0705 mL
	80 mM	0.0621 mL	0.3106 mL	0.6211 mL	1.5529 mL
	100 mM	0.0497 mL	0.2485 mL	0.4969 mL	1.2423 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.