ANO1-IN-1

Name: ANO1-IN-1
Brand: MedChemExpress
SKU: HY-146320
Rating: 4.5 (1 reviews)

Cat. No.: HY-146320 Purity: 99.57%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

ANO1-IN-1 (Compound 9c) is a selective ANO1 channel blocker with an IC₅₀ of 2.56 μM and 15.43 μM against ANO1 and ANO2, respectively. ANO1-IN-1 suppresses strongly proliferation of glioblastoma cells.

For research use only. We do not sell to patients.

ANO1-IN-1 Chemical Structure

CAS No. : 407587-01-3

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	Get quote
200 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE ANO1-IN-1

•Int J Mol Sci. 2024 Sep 5;25(17):9612. [Abstract]

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Biological Activity
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References
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Description

IC₅₀ & Target

IC₅₀: 2.56 μM (ANO1), 15.43 μM (ANO2)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
Astrocyte	GI₅₀	94.83 μM Compound: 9c	Antiproliferative activity against mouse primary astrocytes assessed as reduction in cell viability measured after 72 hrs by Celltiter-Glo luminescent assay Antiproliferative activity against mouse primary astrocytes assessed as reduction in cell viability measured after 72 hrs by Celltiter-Glo luminescent assay	[PMID: 32906067]
U138-MG	GI₅₀	22.24 μM Compound: 9c	Antiproliferative activity against human U138 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay Antiproliferative activity against human U138 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay	[PMID: 32906067]
U-251	GI₅₀	12.58 μM Compound: 9c	Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay	[PMID: 32906067]
U-251	GI₅₀	5.32 μM Compound: 9c	Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability in presence of temozolomide measured after 72 hrs by Celltiter-glo assay Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability in presence of temozolomide measured after 72 hrs by Celltiter-glo assay	[PMID: 32906067]
U-87MG ATCC	GI₅₀	31.49 μM Compound: 9c	Antiproliferative activity against human U87MG cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay Antiproliferative activity against human U87MG cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay	[PMID: 32906067]

In Vitro

ANO1-IN-1 (Compound 9c) (10 μM) significantly suppresses migration and invasion of U251 cells^[1].
ANO1-IN-1 and TMZ combination brings about remarkable synergistic effects in suppressing proliferation of GBM cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

336.49

Formula

C₁₈H₂₈N₂O₂S

CAS No.

407587-01-3

Appearance

Solid

Color

White to off-white

SMILES

CC(C)(C(NC1=C(C2=C(S1)CC(CC2)C(C)(C)C)C(N)=O)=O)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (742.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9719 mL	14.8593 mL	29.7186 mL
5 mM	0.5944 mL	2.9719 mL	5.9437 mL
10 mM	0.2972 mL	1.4859 mL	2.9719 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.57%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (250 KB) HNMR (283 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

References

[1]. Choi SH, et al. Anti-glioma effects of 2-aminothiophene-3-carboxamide derivatives, ANO1 channel blockers. Eur J Med Chem. 2020 Dec 15;208:112688. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9719 mL	14.8593 mL	29.7186 mL	74.2964 mL
	5 mM	0.5944 mL	2.9719 mL	5.9437 mL	14.8593 mL
	10 mM	0.2972 mL	1.4859 mL	2.9719 mL	7.4296 mL
	15 mM	0.1981 mL	0.9906 mL	1.9812 mL	4.9531 mL
	20 mM	0.1486 mL	0.7430 mL	1.4859 mL	3.7148 mL
	25 mM	0.1189 mL	0.5944 mL	1.1887 mL	2.9719 mL
	30 mM	0.0991 mL	0.4953 mL	0.9906 mL	2.4765 mL
	40 mM	0.0743 mL	0.3715 mL	0.7430 mL	1.8574 mL
	50 mM	0.0594 mL	0.2972 mL	0.5944 mL	1.4859 mL
	60 mM	0.0495 mL	0.2477 mL	0.4953 mL	1.2383 mL
	80 mM	0.0371 mL	0.1857 mL	0.3715 mL	0.9287 mL
	100 mM	0.0297 mL	0.1486 mL	0.2972 mL	0.7430 mL

ANO1-IN-1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ANO1-IN-1407587-01-3Chloride ChannelCl− ChannelsANO1 chloride ion channelglioblastomaGBMU87MGU251U138Inhibitorinhibitorinhibit

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ANO1-IN-1

Customer Review

ANO1-IN-1 Related Antibodies

1 Publications Citing Use of MCE ANO1-IN-1

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•Related Screening Libraries:

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•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

ANO1-IN-1 Related Antibodies

Molarity Calculator

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Complete Stock Solution Preparation Table

ANO1-IN-1 Related Classifications

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