2. Membrane Transporter/Ion Channel
  3. Chloride Channel
  4. ANO1-IN-1

ANO1-IN-1 (Compound 9c) is a selective ANO1 channel blocker with an IC50 of 2.56 μM and 15.43 μM against ANO1 and ANO2, respectively. ANO1-IN-1 suppresses strongly proliferation of glioblastoma cells.

For research use only. We do not sell to patients.

ANO1-IN-1 Chemical Structure

ANO1-IN-1 Chemical Structure

CAS No. : 407587-01-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 In-stock
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Solid
5 mg USD 50 In-stock
10 mg USD 70 In-stock
25 mg USD 145 In-stock
50 mg USD 230 In-stock
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

ANO1-IN-1 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE ANO1-IN-1

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

ANO1-IN-1 (Compound 9c) is a selective ANO1 channel blocker with an IC50 of 2.56 μM and 15.43 μM against ANO1 and ANO2, respectively. ANO1-IN-1 suppresses strongly proliferation of glioblastoma cells[1].

IC50 & Target

IC50: 2.56 μM (ANO1), 15.43 μM (ANO2)[1]
Cellular Effect
Cell Line Type Value Description References
Astrocyte GI50
94.83 μM
Compound: 9c
Antiproliferative activity against mouse primary astrocytes assessed as reduction in cell viability measured after 72 hrs by Celltiter-Glo luminescent assay
Antiproliferative activity against mouse primary astrocytes assessed as reduction in cell viability measured after 72 hrs by Celltiter-Glo luminescent assay
[PMID: 32906067]
U138-MG GI50
22.24 μM
Compound: 9c
Antiproliferative activity against human U138 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay
Antiproliferative activity against human U138 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay
[PMID: 32906067]
U-251 GI50
12.58 μM
Compound: 9c
Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay
Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay
[PMID: 32906067]
U-251 GI50
5.32 μM
Compound: 9c
Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability in presence of temozolomide measured after 72 hrs by Celltiter-glo assay
Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability in presence of temozolomide measured after 72 hrs by Celltiter-glo assay
[PMID: 32906067]
U-87MG ATCC GI50
31.49 μM
Compound: 9c
Antiproliferative activity against human U87MG cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay
Antiproliferative activity against human U87MG cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay
[PMID: 32906067]
In Vitro

ANO1-IN-1 (Compound 9c) (10 μM) significantly suppresses migration and invasion of U251 cells[1].
ANO1-IN-1 and TMZ combination brings about remarkable synergistic effects in suppressing proliferation of GBM cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ANO1-IN-1 Related Antibodies
Molecular Weight

336.49

Formula

C18H28N2O2S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(C)(C(NC1=C(C2=C(S1)CC(CC2)C(C)(C)C)C(N)=O)=O)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (742.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9719 mL 14.8593 mL 29.7186 mL
5 mM 0.5944 mL 2.9719 mL 5.9437 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.57%

SDS (393 KB)

COA (250 KB) HNMR (283 KB) LCMS (96 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9719 mL 14.8593 mL 29.7186 mL 74.2964 mL
5 mM 0.5944 mL 2.9719 mL 5.9437 mL 14.8593 mL
10 mM 0.2972 mL 1.4859 mL 2.9719 mL 7.4296 mL
15 mM 0.1981 mL 0.9906 mL 1.9812 mL 4.9531 mL
20 mM 0.1486 mL 0.7430 mL 1.4859 mL 3.7148 mL
25 mM 0.1189 mL 0.5944 mL 1.1887 mL 2.9719 mL
30 mM 0.0991 mL 0.4953 mL 0.9906 mL 2.4765 mL
40 mM 0.0743 mL 0.3715 mL 0.7430 mL 1.8574 mL
50 mM 0.0594 mL 0.2972 mL 0.5944 mL 1.4859 mL
60 mM 0.0495 mL 0.2477 mL 0.4953 mL 1.2383 mL
80 mM 0.0371 mL 0.1857 mL 0.3715 mL 0.9287 mL
100 mM 0.0297 mL 0.1486 mL 0.2972 mL 0.7430 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

ANO1-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ANO1-IN-1407587-01-3Chloride ChannelCl− ChannelsANO1 chloride ion channelglioblastomaGBMU87MGU251U138Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
ANO1-IN-1
Cat. No.:
HY-146320
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.