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CK18 Antibody

Cat. No.: HY-P81181
COA User Guide for Antibodies

CK18 Antibody is an unconjugated, approximately 48 kDa, rabbit-derived, anti-CK18 polyclonal antibody. CK18 Antibody can be used for: WB, ELISA, IHC-P, IHC-F, Flow-Cyt, ICC, IF expriments in human, mouse, rat, and predicted: dog, pig, cow, horse, rabbit background without labeling.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL $70 In-stock
50 μL $190 In-stock
100 μL $300 In-stock
250 μL   Get quote  

* Please select Quantity before adding items.

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Publications Citing Use of MCE CK18 Antibody

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

CK18 Antibody is an unconjugated, approximately 48 kDa, rabbit-derived, anti-CK18 polyclonal antibody. CK18 Antibody can be used for: WB, ELISA, IHC-P, IHC-F, Flow-Cyt, ICC, IF expriments in human, mouse, rat, and predicted: dog, pig, cow, horse, rabbit background without labeling.

Gene ID

3875

Synonyms
K1C18_HUMAN; Keratin, type I cytoskeletal 18; KRT18; CYK18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18 (CK-18); Keratin-18 (K18); PIG46; Keratin 18;
Molecular Weight

Predicted band size: 48kDa

Purity

affinity purified

Subcellular Location

Cytoplasm, perinuclear region. Nucleus, nucleolus.

Conjugation

Non-conjugated

Modification

Unmodified

Product Categories

Primary Antibody; Rabbit Polyclonal Antibody

Clonality

Polyclonal

Host

Rabbit

Reactivity

Human, Mouse, Rat

Predicted Reactivity

Dog, Pig, Cow, Horse, Rabbit

Note: The predicted reactivity is for reference only, not as a product performance guarantee.
SwissProt ID

P05783

Dilution Ratio

WB: 1:500-2000;ELISA: 1:5000-10000;IHC-P: 1:100-500;IHC-F: 1:100-500;Flow-Cyt: 1μg /Test;ICC: 1:100;IF: 1:100-500

  • Western blot analysis of extracts from M-intestine (lane 1) and M-lung (lane 2) using CK18 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
Application

WB, ELISA, IHC-P, IHC-F, FC, ICC/IF

Appearance

Liquid

Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Sensitivity

Endogenous

Immunogen

KLH conjugated synthetic peptide derived from human CK18: 53-150/430

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
CK18 Antibody
Cat. No.:
HY-P81181
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