HRP-conjugated AffiniPure Goat Anti-Rabbit IgG H&L

HRP-conjugated AffiniPure Goat Anti-Rabbit IgG H&L is a HRP-conjugated and Goat origined monoclonal antibody, targeting to Rabbit IgG antibody. HRP-conjugated AffiniPure Goat Anti-Rabbit IgG H&L can binds to the light and heavy chains of Rabbit IgG antibodies, thus can be used for WB, IHC-P, ELISA assays in the background of Rabbit.

HRP

Secondary antibody

Goat

Rabbit

WB: 1:5,000-1:20,000 ;ELISA: 1:5,000-1:20,000; IHC: 1:500-1:1,000

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  Western blot analysis of extracts from Hela(lane 2(20μg) or lane 3(40μg)) and Raji(lane 4(20μg) or lane 5(40μg)), using PARP2 (HY-P80265) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80265, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P, ELISA

Liquid

Stored at -20°C for 1 year.Protect from light. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Synthetic peptide corresponding to Rabbit IgG.