Phospho-Hsp27 (Ser82) Antibody

Phospho-Hsp27 (Ser82) Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 23 kDa, targeting to Phospho-Hsp27 (Ser82). It can be used for WB,IHC-P assays with tag free, in the background of Human.

Hsp27: This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]

Free

3315

Predicted band size: 23 kDa

affinity purified

Non-conjugated

Phosphorylated

Signal Transduction

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human

P04792

WB: 1:500-1:1,000 ;IHC: 1:50-1:100

• 
  Western blot analysis of extracts from Hela(lane 2(20μg) and Hela (lane 3(20μg)), 42℃ treated (2 hours) as indicated, using Phospho-Hsp27 (Ser82) (HY-P80824) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80824, 1/1000) and Loading control antibody (Hsp27, HY-P80709, 1/1000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P

Liquid

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser82 of Human HSP27.The exact sequence is proprietary to MCE.